Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-001-9 | CAS number: 112-73-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-04-30 to 2012-05-03
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with GLP and no defiencies
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Bis(2-butoxyethyl) ether
- EC Number:
- 204-001-9
- EC Name:
- Bis(2-butoxyethyl) ether
- Cas Number:
- 112-73-2
- Molecular formula:
- C12H26O3
- IUPAC Name:
- 1-[2-(2-butoxyethoxy)ethoxy]butane
- Details on test material:
- - Name of test material (as cited in study report): Diethyleneglycoldibutylether
- Physical state: Colourless liquid
- Water solubility: 2.8 g/L (20°C)
- Density: 0.883 g/cm3
- pH: 6.5 - 7.5 (20°C, 100 g/L in isopropanol : water 4 : 1)
- Stability under test conditions: Not specified
- Storage condition of test material: Room temperature, protected from light, in original container
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The concentrations of the test item were analytically determined from all test concentrations and the control after 0 hours (test start) and 72 hours (test end). Samples at test start were taken from an additional replicate without algae. At end of the test samples were taken directly from the pooled test replicates.
All original samples were stored at 6 ± 2 °C until sample preparation, if necessary. Prepared samples were stored at room temperature until analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- APPLICATION
- Method: Stock solution 100 mg/L, freshly prepared with dilution water.
Dispersion treatment Agitation
- Eluate: Dilution water
- Differential loading: 1.00 - 3.16 - 10.0 - 31.6 - 100 mg/L
- Controls: Six replicates without test item were tested under the same test conditions as the test replicates.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Desmodesmus subspicatus Chodat
- Strain: SAG 86.81
- Source (laboratory, culture collection): SAG Pflanzenphysiologisches Institut der Universitaet Goettingen, Nikolausberger Weg 18, D-37073 Goettingen, Germany
- Age of inoculum (at test initiation): 3 days
- Method of cultivation: Fresh stocks were prepared every month on Z-Agar. Light intensity amounted to 35 - 70 µE x m-2 x s-1 for 24 h per day.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- No
Test conditions
- Hardness:
- Dilution water: 0.24 mmol Ca+mg/L.
- Test temperature:
- min.: 22.0 max.: 23.0, mean value: 22.5
- pH:
- Nominal test item concentration pH-value
[mg/L] Start; 0 hours End; 72 hours
100 7.78 7.84
31.6 7.82 8.00
10.0 7.80 8.20
3.16 7.78 8.22
1.00 7.82 7.89
Control 7.80 7.84 - Dissolved oxygen:
- Not measured
- Salinity:
- Not measured, freshwater conditions
- Nominal and measured concentrations:
- See window "Any other information on materials and methods incl. tables"
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flasks
- Type (delete if not applicable): open, cotton wool plugs
- Material, size, headspace, fill volume: sterile 250 mL Erlenmeyer flasks, test volume 100 mL
- Aeration: Test containers were placed on a rotary shaker and oscillated at appr. 70 rpm
- Type of flow-through (e.g. peristaltic or proportional diluter): None
- Renewal rate of test solution (frequency/flow rate): One application at test start
- Initial cells density: Nominal: approximately 2 - 5 x 103 cells/mL, Actual: mean 4910 cells/mL
- Control end cells density: Mean 1713179 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water, composition acc. to the guideline
- Hardness: 0.24 mmol Ca+Mg/L
- pH-value: 8.1 +/- 0.2
- Culture medium: Nutrient Medium Z acc. to LÜTTGE et al. (1994)
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: 24 h
- Light intensity and quality: 60 - 120 µE x m-2 x s-1, mean value: 68.3
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter] fluorimeter, Cell density and self fluorescence was measured daily via Chlorophyll-a- fluorescence, excitation at 436 nm, emission at 685 nm. Dilution water was used as background signal.
TEST CONCENTRATIONS
- Range finding study:Yes
Results of the Range Finding Test (0-72 h)
Nominal Test Item Concentration
[mg/L] Specific Growth Rate Inhibition Yield Inhibition
[%] [%]
2800 100 100
1400 100 100
700 100 100
350 100 100
100 82 99
10.0 4 20
1.00 0 1
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 41.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI 39.5-43.6 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 16.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI 14.4-17.8 mg/L
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test):
- Unusual cell shape: No
- Colour differences: No
- Flocculation: No
- Adherence to test vessels: No
- Aggregation of algal cells: No
- Other: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
EC50 Values of the Reference Item
based on nominal concentrations mg/L, (0-72 hours)
Current Study Valid Range (average ± 3 x SD)
Growth Rate Inhibition
ErC50 0.540 0.686 ± 0.470
95 % confidence interval 0.528 - 0.551
Yield Inhibition
EyC50 0.324 0.319 ± 0.140
95 % confidence interval 0.304 - 0.347
- Reported statistics and error estimates:
- NOEC/LOEC was determined by calculation of statistical significance of growth rates and yield. As standard One Way Analysis of Variance (ANOVA) and DUNNETT’s Test were used for NOEC/LOEC calculations. When running a One Way Analysis of Variance a Normality test and an Equal Variance test were done first. The SHAPIRO-WILK-Test was used to test for normally distributed populations. The Levene median test was used for equal variance. P-values for both Normality and Equal Variance test were 0.05. The a-value (acceptable probability of incorrectly concluding that there is a difference) is a=0.05.
Any other information on results incl. tables
Cell Densities
|
Nominal test item concentration |
Replicate |
Cell density [cells/mL] |
||||||
[mg/L] |
No. |
0 hours |
24 hours |
48 hours |
72 hours |
|
|||
100 |
1 |
4910 |
9713 |
9151 |
7902 |
|
|||
2 |
4910 |
11317 |
8762 |
7959 |
|
||||
3 |
4910 |
11534 |
9357 |
7262 |
|
||||
Mean |
4910 |
10855 |
9090 |
7708 |
|
||||
31.6 |
1 |
4910 |
17156 |
48881 |
208842 |
|
|||
2 |
4910 |
18636 |
53664 |
218449 |
|
||||
3 |
4910 |
20304 |
66906 |
242658 |
|
||||
Mean |
4910 |
18699 |
56484 |
223316 |
|
||||
10.0 |
1 |
4910 |
35628 |
237073 |
1272260 |
|
|||
2 |
4910 |
34109 |
209472 |
1409082 |
|
||||
3 |
4910 |
32589 |
229579 |
1576030 |
|
||||
Mean |
4910 |
34109 |
225375 |
1419124 |
|
||||
3.16 |
1 |
4910 |
29207 |
244276 |
1687803 |
|
|||
2 |
4910 |
27213 |
245806 |
1810001 |
|
||||
3 |
4910 |
27230 |
272501 |
1897402 |
|
||||
Mean |
4910 |
27883 |
254194 |
1798402 |
|
||||
1.00 |
1 |
4910 |
31150 |
260755 |
1719013 |
|
|||
2 |
4910 |
29684 |
239605 |
1845408 |
|
||||
3 |
4910 |
32734 |
244960 |
1750493 |
|
||||
Mean |
4910 |
31189 |
248440 |
1771638 |
|
||||
Control |
1 |
4910 |
37456 |
274159 |
1705743 |
|
|||
2 |
4910 |
32883 |
247052 |
1561610 |
|
||||
3 |
4910 |
34714 |
239646 |
1877634 |
|
||||
4 |
4910 |
37233 |
324542 |
1812709 |
|
||||
5 |
4910 |
30930 |
250200 |
1693557 |
|
||||
6 |
4910 |
32352 |
241839 |
1627821 |
|
||||
Mean |
4910 |
34261 |
262906 |
1713179 |
|
Evaluation after 72 hours
Statistically significant differences of growth rates and yield compared to
control values are marked (+), not significant differences are marked (-).
Nominal test item concentration |
Replicate |
Growth rate |
Rate-related inhibition |
Yield |
Inhibition of yield |
||
[mg/L] |
No. |
[d-1] |
[%] |
[cells/mL] |
[%] |
||
100 |
1 |
0.159 |
91.9 |
2992 |
99.8 |
||
2 |
0.161 |
91.8 |
3049 |
99.8 |
|||
3 |
0.130 |
93.3 |
2352 |
99.9 |
|||
Mean |
(+) |
0.150 |
92.3 |
(+) |
2798 |
99.8 |
|
31.6 |
1 |
1.25 |
35.9 |
203932 |
88.1 |
||
2 |
1.27 |
35.2 |
213539 |
87.5 |
|||
3 |
1.30 |
33.4 |
237748 |
86.1 |
|||
Mean |
(+) |
1.27 |
34.8 |
(+) |
218406 |
87.2 |
|
10.0 |
1 |
1.85 |
5.05 |
1267350 |
25.8 |
||
2 |
1.89 |
3.31 |
1404172 |
17.8 |
|||
3 |
1.92 |
1.39 |
1571120 |
8.03 |
|||
Mean |
(+)1 |
1.89 |
3.25 |
(+) |
1414214 |
17.2 |
|
3.16 |
1 |
1.95 |
0.22 |
1682893 |
1.49 |
||
2 |
1.97 |
-0.97 |
1805091 |
-5.67 |
|||
3 |
1.99 |
-1.78 |
1892492 |
-10.8 |
|||
Mean |
(-) |
1.97 |
-0.84 |
(-) |
1793492 |
-4.99 |
|
1.00 |
1 |
1.95 |
-0.09 |
1714103 |
-0.34 |
||
2 |
1.98 |
-1.3 |
1840498 |
-7.74 |
|||
3 |
1.96 |
-0.4 |
1745583 |
-2.18 |
|||
Mean |
(-) |
1.96 |
-0.60 |
(-) |
1766728 |
-3.42 |
|
Control |
1 |
1.95 |
1700833 |
||||
2 |
1.92 |
1556700 |
|||||
3 |
1.98 |
1872724 |
|||||
4 |
1.97 |
1807799 |
|||||
5 |
1.95 |
1688647 |
|||||
6 |
1.94 |
1622911 |
|||||
Mean |
1.95 |
1708269 |
Section-by-Section and Average Specific Growth Rates of the Control Group
(0 – 72 hours)
Replicate No. |
Specific growth rate [d-1] |
Mean (0 - 72 h) |
SD ± |
CV |
Mean CV [%] |
|||
section-by-section |
||||||||
0 - 24 hours |
24 - 48 hours |
48 - 72 hours |
||||||
Control |
1 |
2.03 |
1.99 |
1.83 |
1.95 |
0.108 |
5.53 |
5.86 |
2 |
1.90 |
2.02 |
1.84 |
1.92 |
0.0879 |
4.58 |
||
3 |
1.956 |
1.93 |
2.06 |
1.98 |
0.0673 |
3.39 |
||
4 |
2.03 |
2.17 |
1.72 |
1.97 |
0.228 |
11.6 |
||
5 |
1.84 |
2.09 |
1.91 |
1.95 |
0.129 |
6.61 |
||
6 |
1.89 |
2.01 |
1.91 |
1.94 |
0.0675 |
3.49 |
||
Mean |
1.95 |
|
||||||
SD ± |
0.02 |
|
||||||
CV [%] |
1.16 |
|
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study Diethyleneglycoldibutylether was found to inhibit the growth of the freshwater green alga Desmodesmus subspicatus after 72 hours. The NOEC-values for inhibition of specific growth rate and inhibition of yield after 72 hours were 10.0 mg/L and 3.16 mg/L, respectively. The LOEC-values for inhibition of specific growth rate and inhibition of yield after 72 hours were 31.6 mg/L and 10.0 mg/L, respectively. The EC50-values for inhibition of specific growth rate (ErC50) and yield (EyC50) with 95 % Confidence Intervals after 72 hours were 41.3 (39.5 – 43.6) and 16.4 (14.6 – 18.8) mg/L, respectively.
All effect levels are given based on nominal concentrations of Diethyleneglycoldibutylether. - Executive summary:
The toxicity of Diethyleneglycoldibutylether (batch number: DEG4136740) to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD 201 at Dr.U.Noack-Laboratorienin 31157 Sarstedt, Germany from 2012-04-30 to 2012-05-03. The aim of the study was to assess the effects on growth rate and yield over a period of 72 hours.
The study was conducted under static conditions with an initial cell density of 4910 cells/mL. Based on a preliminary test 5 concentration levels were tested in a geometrical series with a nominal dilution factor of : 1.00 - 3.16 - 10.0 - 31.6 - 100 mg/L. Three replicates were tested for each test item concentration and six replicates for the control. Environmental conditions were determined to be within the acceptable limits.
The concentrations of Diethyleneglycoldibutyletherwere analytically verified by GC-MS at test start and test end of all test concentrations and the control. The measured concentrations of Diethyleneglycoldibutyletherat test start were in the range of 102 to 115 % and after 72 hours in the range of 89 to 112 % of the nominal values. Therefore, all effect values given are based on the nominal test item concentrations of the test item Diethyleneglycoldibutylether.
NOEC, LOEC, EC - values and 95 % Confidence Intervals of Diethyleneglycoldibutylether
(0-72 hours)based on nominal test item concentrations [mg/L]
Growth Rate Inhibition
NOEC
10.0
LOEC
31.6
ErC10
16.1 (14.4 – 17.8)
ErC20
22.8 (21.4 – 23.9)
ErC50
41.3 (39.5 – 43.6)
Inhibition of Yield
NOEC
3.16
LOEC
10.0
EyC10
8.48 (7.25 – 9.83)
EyC20
10.6 (9.37 – 12.0)
EyC50
16.4 (14.6 – 18.8)
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.