Acetone oxime

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

GLP compliance:

yes

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Acetone oxime

EC Number:

204-820-1

EC Name:

Acetone oxime

Cas Number:

127-06-0

Molecular formula:

C3H7NO

IUPAC Name:

acetone oxime

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Administration / exposure

Type of coverage:

occlusive

Vehicle:

water

Duration of exposure:

24 hours

Doses:

0, 100, 500 and 1000 mg/kg

No. of animals per sex per dose:

5 per sex per dose

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 15 days
- Frequency of observations: 1,2 and 4 hours after dosing and then daily
- Frequency of weighing: Body weights were obtained prior to dosing and on days 1, 4, 7, 11 and 15.
- Necropsy of survivors performed: yes
- Other examinations performed: neurological examinations, haematology, organ weights, histopathology

Statistics:

Body weights, haematology data, organ weights and organ/body weight rations were analysed statistically.

Results and discussion

Preliminary study:

In a preliminary study 1/2 animals died at 1000 mg/kg and 2/2 animals died at 2000 mg/kg

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 1 000 mg/kg bw

Based on:

act. ingr.

Mortality:

No animals died in the main study

Clinical signs:

At 1000 mg/kg hypoactivity, fecal staining and a darkened iris were seen following dosing and poor food consumption on Day 3. At the lower doses only fecal staining was observed. All animals free of clinical signs from Day 4 of the study. Neurological examination revealed no unusual signs in any animal.

Body weight:

Body weights of test animals were comparable to controls.

Gross pathology:

No effects noted on organ weights or gross pathology. Microscopic pathology revealed myeloid and erythroid hypercellularity of the femoral bone marrow in all top dose animals and 4/10 mid dose animals.

Other findings:

Dose related methaemogloburia was noted on days 1 and/or 5 but not at study termination. Marked differences were noted in most haematology parameters in the top dose animals.

Applicant's summary and conclusion

Interpretation of results:

harmful

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The dermal LD50 for acetone oxime is > 1000 mg/kg