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Diss Factsheets
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EC number: 237-358-4 | CAS number: 13762-14-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well documented publication which meets basic scientific principles.
Data source
Reference
- Reference Type:
- publication
- Title:
- Detection of contact sensitivity of metal salts using the murine local lymph node assay.
- Author:
- Ikarashi, Y. et al.
- Year:
- 1 992
- Bibliographic source:
- Toxicology Letters 62: 53-61
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Deviations:
- yes
- Remarks:
- - Radiolabelling of cells was performed ex vivo, different vehicles and various vehicle concentrations were compared.
- GLP compliance:
- not specified
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- 7791-13-1
- EC Number:
- 616-574-6
- Cas Number:
- 7791-13-1
- IUPAC Name:
- 7791-13-1
- Reference substance name:
- cobalt(II)chloride hexahydrate
- IUPAC Name:
- cobalt(II)chloride hexahydrate
- Details on test material:
- - Name of test material (as cited in study report): cobalt(II)chloride hexahydrate
- Molecular formula (if other than submission substance): CoCl2*6H2O
- Molecular weight (if other than submission substance): 237.84 g/mol
- Analytical purity: no data
- Other: Source: Wako Pure Chemical Industries, Ltd., Osaka, Japan
Constituent 1
Constituent 2
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- Balb/c
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: Japan SLC Inc., Shizuoka, Japan
- Age at study initiation: 6-8 weeks
Study design: in vivo (LLNA)
- Vehicle:
- other: DMSO or 70% ethanol
- Concentration:
- 5% of CoCl2*6H2O in DMSO, 5% of CoCl2*6H2O in ethanol
- No. of animals per dose:
- 3
- Details on study design:
- MAIN STUDY
ANIMAL ASSIGNMENT AND TREATMENT
- Criteria used to consider a positive response: SI > 2
TREATMENT PREPARATION AND ADMINISTRATION:
Exp 1: Groups of mice were treated with 5% of CoCl2*6H2O in DMSO or DMSO alone by applying 25 µL to the dorsum of both ears for three consecutive days. Four days following the initial application, draining lymph nodes were excised. After preparation of a single cell suspensions, the incorporation of [³H]TdR was performed for 18 hours ex vivo.
Exp 2: Groups of mice were treated with 5% of CoCl2*6H2O in DMSO or with 70% ethanol alone by applying 25 µL to the dorsum of both ears for three consecutive days. Prior to the treatment, the ears of each mouse were gently abraded using a 19-g needle. Four days following the initial application, draining lymph nodes were excised. A single cell suspension of LNC was prepared and incorporation of [³H]TdR was measured.
In both experiments a single cell suspension of lymph node cells was prepared and incorporation of [³H]TdR was measured after 18 hours incubation. - Positive control substance(s):
- not specified
- Statistics:
- [³H]TdR incorporation was calculated as mean cpm ± SD. Stimulation indices of lymph node cell proliferation between substance-treated and vehicle-treated groups were calculated.
Results and discussion
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Remarks on result:
- other: Exp 1: 5% CoCl2*6H2O in DMSO: 4.33 Exp 2: without abrasion: 5% CoCl2*6H2O 70% ethanol: 2.1 with abrasion: 5% CoCl2*6H2O in 70% ethanol: 4.37
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: see Remark
- Remarks:
- In mean cpm ± SD (x0.001) per node of 3 animals: Exp 1: DMSO: 1.71 ± 0.68 mean cpm ± SD (x0.001); 5% CoCl2*6H2O in DMSO: 7.42 ± 1.00 mean cpm ± SD (x0.001) Exp 2: 70% ethanol: 2.25 ± 0.30 mean cpm ± SD (x0.001); without abrasion: 5% CoCl2*6H2O in 70% ethanol: 4.72 ± 0.52 mean cpm ± SD (x0.001) with abrasion: 5% CoCl2*6H2O in 70% ethanol: 9.84 ± 2.76 mean cpm ± SD (x0.001)
Any other information on results incl. tables
Exp 1:
Repeated exposure for three consecutive days induced an increase of LNC proliferative response in the draining lymph node (SI=4.33).
Exp 2:
Dissolution in 70% ethanol resulted in a greater proliferative response in the abrasion group (SI=4.37) than in the non-abrasion group (SI=2.1). The ear abrasion is effective in enhancing the penetration of the metal ion and to increase the LNC proliferation. However, the abrasion technique does not affect the LNC response in the vehicle or non-sensitiser-treated group.
Applicant's summary and conclusion
- Interpretation of results:
- sensitising
- Remarks:
- Migrated information
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