Propanenitrile, 3,3'-(dodecylimino)bis-

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

other information

Study period:

1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study under GLP with minor deviations

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

yes

Principles of method if other than guideline:

Reference substance: copper(II)sulfate-pentahydrate instead of 3,5-dichlorophenol due to carciogenic properties
Sludge was washed 2x instead of 3x. This has been proved to be sufficient.
Dry weight of sludge: 3g/l±20% instead of 4g/l±10% due to high activity of activated sludge from sewage plant Hildesheim.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3-[(2-cyanoethyl)(dodecyl)amino]propanenitrile

EC Number:

605-014-6

Cas Number:

1555-62-0

Molecular formula:

C18H33N3

IUPAC Name:

3-[(2-cyanoethyl)(dodecyl)amino]propanenitrile

Sampling and analysis

Details on sampling:

Ultrasound dispersing: 30 min at 40°C.
Test concentrations: 32 - 100 - 320-1000 - 3200 - 10000 [mg / L]
Single stock solution. No carrier was used.
200 mL inoculum + 16 ml synthetic wastewater + 284 mL aqua.demin. + test substance

Test solutions

Details on test solutions:

Ultrasound dispersing: 30 min at 40°C.
Test concentrations: 32 - 100 - 320-1000 - 3200 - 10000 [mg / L]
Single stock solution. No carrier was used.
200 mL inoculum + 16 ml synthetic wastewater + 284 mL aqua.demin. + test substance

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

Origin: sewage plant Hildesheim, clarifier
The activated sludge was washed 2x with dechlorinated tap water (autoclaved).
dry weight: 3 g/l

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test conditions

Test temperature:

18-22°C

pH:

7,56

Dissolved oxygen:

permanent ventilation through aquarium pumps

Nominal and measured concentrations:

32 - 100 - 320-1000 - 3200 - 10000 [mg / L]

Details on test conditions:

The respiration rates were determined after 3-hour contact time aby determination of the inhibitory effect of the test concentrations compared to the control system.
Concentrations: 10 - 32 - 100 - 320 - 1000 [mg/L].
Concentrations of referece substance: 58 - 100 - 180 [mg/L]

Reference substance (positive control):

yes

Remarks:

Copper(ll)sulfate-pentahydrate (p.a., >95%)

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

960 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: 127

Effect concentrations 2

Duration:

3 h

Dose descriptor:

other: EC20

Effect conc.:

215 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

3 h

Dose descriptor:

other: EC80

Effect conc.:

4 289 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

EC50 of reference substance = 99 [mg/L]

Reported statistics and error estimates:

The deviation of control respiratory rates was 11 [%].

Any other information on results incl. tables

	concentration (mg/L)	respiratory rates [mg 02/L h]	inhibition (%)
Substance	10000	3.2	90
	3200	7.6	76
	1000	17.6	45
	320	24.0	25
	100	23.6	26
	32	31.6	1
Control K1	0	30.0	0
Control K2	0	33.6	0
Reference substance	180	6.4	80
	100	15.6	51
	58	24.8	22

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

N,N-Di-(2-cyanoethyl)-dodecylnitril shows in concentrations <215 [mg / L] no toxic effect to bacteria in activated sludge of a municipal wastewater treatment plant according to OECD209.
EC50=960mg/l

Executive summary:

N,N-Di-(2-cyanoethyl)-dodecylnitril shows in concentrations <215 [mg / L] no toxic effect to bacteria in activated sludge of a municipal wastewater treatment plant according to OECD209.

EC50=960mg/l