Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 231-106-7 | CAS number: 7439-97-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- no data available
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well documented publication.
Data source
Reference
- Reference Type:
- publication
- Title:
- Mutagenicity of metal salts in the L5178Y mouse lymphoma assay.
- Author:
- Oberly, T.J.; et al.
- Year:
- 1 982
- Bibliographic source:
- Journal of Toxicology and Environmental Health, 9:367–376.
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Forward mutation assay at the thymidine kinase locus (TK+/-) in L5178Y mouse lymphoma cells.
- GLP compliance:
- no
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- Mercury chloride
- EC Number:
- 231-430-9
- EC Name:
- Mercury chloride
- Cas Number:
- 7546-30-7
- IUPAC Name:
- mercury dichloride
- Reference substance name:
- Mercury dichloride
- EC Number:
- 231-299-8
- EC Name:
- Mercury dichloride
- Cas Number:
- 7487-94-7
- IUPAC Name:
- mercury dichloride
- Details on test material:
- - Name of test material (as cited in study report): mercury chloride
- Molecular formula (if other than submission substance): HgCl2
- Physical state: solid
No further details are given.
Constituent 1
Constituent 2
Method
- Target gene:
- TK+/-
Species / strain
- Species / strain / cell type:
- mouse lymphoma L5178Y cells
- Details on mammalian cell type (if applicable):
- - Type and identity of media: all cells were thawed from frozen stock and maintained in Fisher's medium for leukemic cells of mice containing 10% heat-inactivated horse serum, Pluronic F68, sodium pyruvate, penicillin G and streptomycin sulfate.
- Properly maintained: yes.
- Periodically checked for Mycoplasma contamination: no data.
- Periodically checked for karyotype stability: no data.
- Periodically "cleansed" against high spontaneous background: yes, background spontaneous TK-/- mutant frequencies were reduced weekly by 24-hour treatment of the cells with medium containing thymidine, hypoxanthene, methotrexate and glycine. - Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- 2, 4, 6 and 8 µg/mL
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: sterile glass-distilled water
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 2-acetylaminofluorene
- Remarks:
- with metabolic activation
Migrated to IUCLID6: 50 µg/mL
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium and plated on agar
- 0.1 mL of metal solution was added to a 10-mL suspension containing 6 * 10^6 cells from culture recently cleansed of TK-/- cells.
- When testing with metabolic activation, the 10-mL suspension included 4 mL of S9 mix.
DURATION
- Exposure duration: 4 hours at 37°C
- Expression time (cells in growth medium): after exposure, the cells were washed twice, fresh medium was added, and the cultures were carried throgh a 2-day expression period.
- Selection time (if incubation with a selection agent): 12 days after the end of expression time (approximately 2 weeks). On day 2, a sample of each culture was centrifuged and the cells resuspended in Fischer's medium. The concentrated cells were diluted and appropriate dilutions plated in clonig medium with and without TFT.
- Cells were also cloned on nonselective plates for each test and control tube.
SELECTION AGENT (mutation assays):
- 2 µg/mL TFT (trifluorothymidine)
NUMBER OF REPLICATIONS:
- plated in triplicate
COLONIE COUNTS:
- A New Brunswick Scientific automatic colony counter was used to determine the number of colonies per plate.
DETERMINATION OF CYTOTOXICITY
- Method: cloning efficiency; relative total growth: viable cells were determined by trypan blue staining.
- Total survival was determined by a combination of growth in suspension culture and soft cloning efficiency data.
OTHER:
- The mutation frequency (MF) was calculated as the number of mutants per 10^5 colony-forming cells. - Evaluation criteria:
- no data
- Statistics:
- no data
Results and discussion
Test results
- Species / strain:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with
- Genotoxicity:
- positive
- Remarks:
- weak positive response at 8 and 6 µg/mL
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Remarks:
- Total survival at 8 and 6 µg/mL was well above the cutoff level of 10%.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- - Mutation frequencies determined for HgCl2 were 12.5, 16.4, 19.5 and 31.9 for the tested concentrations of 2, 4, 6 and 8 µg/mL, respectively.
- Mutation frequency of the positive control was increased 27.8-fold over the solvent control.
- Mutation frequency of the test substance was increased 1.4, 1.8, 2.1 and 3.5-fold over the solvent control for the tested concentrations of 2, 4, 6 and 8 µg/mL, respectively.
TEST-SPECIFIC CONFOUNDING FACTORS: no data
RANGE-FINDING/SCREENING STUDIES: no data
COMPARISON WITH HISTORICAL CONTROL DATA:
- Values for the solvent and positive controls fall within the range established by previous experiments of the testing laboratory.
ADDITIONAL INFORMATION ON CYTOTOXICITY:
- Percent of cell survival at the doses that evoke weak mutagenic potential (8 and 6 µg/mL) was 24 and 56%, respectively, compared to control. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive with metabolic activation
Under the test conditions reported, the test item mercury chloride showed a weak mutagenic activity in mouse lymphoma L5178Y cells in the presence of a metabolic activation system. - Executive summary:
Forward mutation assay at the thymidine kinase locus (TK+/-) was performed in L5178Y mouse lymphoma cells at concentrations of 2 -8 µg/mL in the presence of metabolic activation. The results showed that the test item mercury chloride was weakly mutagenic in mouse lymphoma L5178Y cells in the presence of a metabolic activation system at the two highest tested concentrations (8 and 6 µg/mL).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.