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EC number: 600-704-3 | CAS number: 10597-60-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 7th April 2020 to 28th April 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 020
- Report date:
- 2020
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4-(2-hydroxyethyl)benzene-1,2-diol
- EC Number:
- 600-704-3
- Cas Number:
- 10597-60-1
- Molecular formula:
- C8H10O3
- IUPAC Name:
- 4-(2-hydroxyethyl)benzene-1,2-diol
1
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- HPLC-UV
- Details on sampling:
- Analysis frequency: in 0-hour fresh solution and 48-hour old solution
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Method of preparation of stock solution:
Range Finding Test
A solution at 1000 mg/L of the test item was prepared in culture medium. Then 5 different concentrations were prepared, with ten-fold dilutions from 1000 mg/L to 0.01 mg/L.
Definitive Test
Method of preparation of test solutions:
After the range finding test a solution at 1000 mg/L of the test item has been prepared in culture medium. Then 5 dilutions have been performed in culture medium in order to obtain the diluted nominal solutions 1.25, 2.50, 5.0, 10.0 and 20.0 mg/L.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: straus
- Age at study initiation: 6-24 hours
- Source: Breeder in Eurofins Biolab Test Facility
- Feeding during test: None
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- 24hr, 48 hr
Test conditions
- Hardness:
- between 140 and 250 mg CaCO3/L
- Test temperature:
- 20°C ± 2°C
- Dissolved oxygen:
- >3 mg/L
- Nominal and measured concentrations:
- Based on a preliminary test, the test concentrations were used as follows:
Nominal amount of test item A solution of 1000 mg/L was prepared in culture medium.
Then five nominal concentrations were prepared 1.25, 2.5, 5.0, 10.0 and 20.0 mg/L.
Measured concentrations: the determined concentrations in fresh media were 1.18, 2.41, 4.79, 9.55 and 19.28 mg/L. The determined concentrations in 48 hours old media were 0.00, 0.00, 0.67, 1.61 and 4.64 mg/L.
The sample is considered not stable because the measured concentration of the test item at the end of the test has not been maintained within + 20% to the concentration at the measured concentrations at the beginning of the test. - Details on test conditions:
- TEST SYSTEM
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water has been used; it has been prepared dissolving the following salts in 1 litre of deionised water: bihydrated calcium chloride 11.76 g/L, eptahydrated magnesium sulphate 4.93 g/L, acid sodium bicarbonate 2.59 g/l and potassium chloride 0.23 g/L.
OTHER TEST CONDITIONS
- pH: 7.60 - 8.06
- Photoperiod: test carried out in darkness
- Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 10.749 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The sample is considered not stable because the measured concentration of the test item at the end of the test has not been maintained within + 20 %to the concentration at the measured concentrations at the beginning of the test.
As the test item was not stable the statistical analysis (EC50) has been performed using the measured concentrations at T=0.
There were no immobilised daphnia in any of the control replicates - Reported statistics and error estimates:
- The EC50 was evaluated using EPA Probit Analysis Program Version 1.5.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The obtained results, in compliance with assay validity criteria, showed that Daphnia magna EC50 after 48 hours of the measured beginning concentration of the test item HYDROXYTYROSOL is equal to 10.749 mg/L.
- Executive summary:
The aim of the study is to determine the ecotoxicological effects of the test item “HYDROXYTYROSOL”on biotic systems: in particular the aquatic organism Daphnia magna was used as test system in order to calculate the EC50 value.
The toxicity of the test item, HYDROXYTYROSOL, on the cladocero Daphnia magna was evaluated according to OECD guideline No. 202.
After a range finding test, the organisms were exposed to various dilutions of the test item for a total period of 48 hours.
Daphnia were kept under observation for a period of 48 hours and the number of immobilised organisms and/or possible abnormal behaviours both in the control and in the treatment group vessel were observed.
At the beginning and at the end of the test (48 hours) dissolved oxygen and pH were measured in the highest concentration and in the control group. The temperature of the assay environment was recorded at the beginning and at the end of the test.
The stability of the test item in the definitive test was performed using HPLC equipped with a UV detector according to method described in STULV20AA0627-1 GLP - MdP.
The test item was measured at the beginning and at the end of the test in the control and in the treated samples.
The obtained results, in compliance with test acceptability criteria, of the nominal concentration of the test item can be summarised as follow:
EC50 of the test item at T= 48 h (measured concentration at the beginning of the test) = 10.749 mg/L
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