Acetamide, N-(2-amino-4,5,6,7-tetrahydro-6-benzothiazolyl)-, hydrobromide (1:1)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July 2004 to October 2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2004

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

yes

Remarks:

The following deviations were stated and signed by the study director on Oct. 8th 2004: ◆ The effluent was added to the test medium immediately after the preparation. To minimize the O2-consumption of the control, the medium was then aerated for four days

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

N-(2-Methyl-4,5,6,7-tetrahydro-benzothiazol-6-yl)-acetamide hydrobromide

EC Number:

600-588-4

Cas Number:

104617-50-7

Molecular formula:

C9 H13 O N3 S x HBr

IUPAC Name:

N-(2-Methyl-4,5,6,7-tetrahydro-benzothiazol-6-yl)-acetamide hydrobromide

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic, non-adapted

Duration of test (contact time):

28 d

Details on study design:

The test item was tested using a concentration of 2.43 mg/L, (corresponding to a theo­retical oxygen consumption of 6.32 mg/L) in test medium following OECD 301D and EU­ Guideline C.4-E.
Sodium benzoate was chosen as reference item.
Effluent from a sewage

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

ca. 0

Sampling time:

28 d

Details on results:

For the determination of the results, the data were evaluated with and without considera­tion of nitrification.
The degree of biodegradation was 0 % after 28 days. A 10-day-window could not be detected.
The test item SND 919 Precursor 1 (Thiazol) is not readily biodegradable.


The validity criteria were met.
The slightly lower O2-uptake of the test vessels in comparison with the control vessels caused negative degradation values for the test item. This is probably due to the fact that the measurement errors of test vessels and control vessels add up when using the equa­ tion proposed in the guideline. This might also be due to different survival rates of the inoculum in the test flasks although the test item didn't show toxic effect in the toxicity vessels.
As degradation in the toxicity flask was more than 25 % at the end of the test, the test item can be stated as not toxic towards the inoculum in a concentration of 2.43 mg/L. Therefore, the result of the test can be considered valid.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The test item SND 919 Precursor 1 (Thiazol) is not readily biodegradable.