Fatty acids, tall-oil, compds. with diethanolamine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From May 6, 1997 to May 10, 1997

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

No analytical dose verification

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

; no details on validity criteria is available. No analysis of the test solutions was peformed; but this deviation can be considered acceptable as the test substance is water soluble.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION:
- Method: 2 gm of the test substance was dispersed in reverse osmosis water using ultrasonification. The volume was adjusted to 1 L to give 2 gm/L stock concentration. A serial dilution was done to prepare a 200 mg/L stock. Aliquots of (10 and 30 mL) from 200 mg/L stock disepersion and aliquots (10, 30, 100 and 300 mL) from 2 gm/L stock were separately dispersed in dechlorinated tap water and the volume was finally adjusted to 2 L to give 0.1, 0.3, 1.0, 3.0, 10.0, 30.0 mg/l concentrations, respectively.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Strain: Oncorhynchus mykiss
- Source: Parkwood trout farm, Harrietsham, Kent, UK
- Length at study initiation (length definition, mean, range and SD): 4.2 ± 0.2 cm
- Weight at study initiation (mean and range, SD): 1.0 ± 0.14 g

ACCLIMATION
- Acclimation period: 7d
- Health during acclimation (any mortality observed): Approximately 4% mortality
- Medication: None

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

100 mg/L CaCO3

Test temperature:

14 ± 0.1°C

Nominal and measured concentrations:

- Nominal: 0.10, 0.30, 1.0, 3.0, 10.0 and 30.0 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 L vessels
- Aeration: Yes
- No. of organisms per vessel: 10
- Renewal rate of test solution (frequency/flow rate): None


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Laboratory tap water was dechlorinated by passage through an activated carbon filter (Purit series 500) and partly softened (Elga Nimbus 1248D duplex water softener). Water was then passed through a series of computer controlled plate heat exchangers to achieve the required temperature
- Hardness: 100 mg/L CaCO3


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality rates, swimming at the bottom and surface of test vessel, loss of equilibrium and presence of moribund fish were observed at 3, 6, 24, 48, 72 and 96 h.

TEST CONCENTRATIONS
- Test concentrations: Nominal: 0.10, 0.30, 1.0, 3.0, 10.0, 30.0 mg/L

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 1.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: (95% CI: 0.84-1.7 mg/L)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

ca. 0.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

ca. 3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Sub-lethal effects: No abnormalities observed up to the concentration of 0.30 mg/L. Swimming at the bottom and surface of test vessel, loss of equilibrium, presence of moribund fish and mortality were observed at the concentrations >1.0 mg/L at 3, 6, 24, 48, 72 and 96 h.
- Mortality of control: No
- Effect concentrations exceeding solubility of substance in test medium: The test media at the concentrations of 0.10, 0.30, 1.0 and 3.0 mg/L were clear colorless solutions and the test media at the concentrations of 10 and 30 mg/L were opaque dispersions.

Sublethal observations / clinical signs:

Mortality was determined based on moving average method by Thompson, 1974.

Validity criteria fulfilled:

not specified

Conclusions:

Under the study conditions, analysis of the mortality data by the moving average method of Thompson (1947) yielded a nominal 96 h LC50 value of 1.2 mg/L. The nominal 96 h NOEC and LC100 were determined at 0.3 and 3.0 mg/L, respectively.

Executive summary:

A study was conducted to assess the acute toxicity of the test substance, C16-18 and C18-unsatd. DEA, to fish (Rainbow trout) according to the OECD Guideline 203. Ten fish per test vessel were exposed to the test substance (dispersed in water using ultrasonication) at the concentrations of 0.1, 0.3, 1.0, 3.0, 10.0 and 30.0 mg/L for 96 h under static conditions. Mortality and other sub-lethal effects such swimming at the bottom and surface, loss of equilibrium and morbidity were recorded at 3, 6, 24, 48, 72 and 96 h. No analytical dose verification was conducted. Under the study conditions, analysis of the mortality data by the moving average method of Thompson (1947) yielded a nominal 96 h LC50 value of 1.2 mg/L. The nominal 96 h NOEC and LC100 were determined at 0.3 and 3.0 mg/L, respectively (Wetton 1997).

Description of key information

A study was conducted to assess the acute toxicity of the test substance, C16-18 and C18-unsatd. DEA,to fish (Rainbow trout) according to the OECD Guideline 203. Ten fish per test vessel were exposed to the test substance (dispersed in water using ultrasonication) at the concentrations of 0.1, 0.3, 1.0, 3.0, 10.0 and 30.0 mg/L for 96 h under static conditions. Mortality and other sub-lethal effects such swimming at the bottom and surface, loss of equilibrium and morbidity were recorded at 3, 6, 24, 48, 72 and 96 h. No analytical dose verification was conducted. Under the study conditions, analysis of the mortality data by the moving average method of Thompson (1947) yielded a nominal 96 h LC50 value of 1.2 mg/L. The nominal 96 h NOEC and LC100 were determined at 0.3 and 3.0 mg/L, respectively (Wetton 1997).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

1.2 mg/L