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EC number: 863-757-3 | CAS number: 1445866-55-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 Jan - 28 Jan 2022
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 022
- Report date:
- 2022
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- adopted April 13, 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1-(2-bromo-4-chlorophenyl)-4-(trifluoromethyl)-1H-1,2,3-triazole
- EC Number:
- 863-757-3
- Cas Number:
- 1445866-55-6
- Molecular formula:
- C9H4BrClF3N3
- IUPAC Name:
- 1-(2-bromo-4-chlorophenyl)-4-(trifluoromethyl)-1H-1,2,3-triazole
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: Duplicate samples from the freshly prepared test media of all test concentrations and the control were taken at the start of the test.
For the determination of the stability of the test item under the test conditions and of the maintenance of the test item concentrations during the test period, duplicate samples from the test media of all test concentrations and the control were collected at the end of the test (after 48 hours) by pouring together the contents of the test beakers of each treatment. All samples were diluted by a factor of 2 with acetonitrile. Additional samples of the untreated controls and the sample dilution solvent were taken at each sampling test start and test end without any sample treatment.
For the determination of the stability of the test item under the test conditions and of the maintenance of the test item concentrations during the test period.
- Sample storage conditions before analysis: deep frozen
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
The test item is not well soluble in test medium. To avoid physical effects of undissolved test item on the daphnids, no concentrations above the solubility limit of the test item in test water was tested. Therefore, a supersaturated stock solution with a loading rate of nominal 100 mg test item/L was prepared by suspending 103.2 mg test item in 1032 mL test water. The stock suspension was stirred for 24 hours at room temperature in the dark to dissolve as much test item as possible. Then, non-dissolved fractions of the test item were separated from the test medium by membrane filtration (0.45 µm cellulose acetate filter). The solution with dissolved test item was used as the test medium of the highest test concentration and to prepare the desired 1:3.3, 1:10.9, 1:35.9 and 1:118.6 dilutions.
The test media were prepared just before introduction of the daphnids (= start of the test).
In the control, test water was used without addition of the test item.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
Daphnia magna (Straus), clone 5
Age at Test Start: From 0.75 to 18.25 hours old
Source: The daphnids introduced in the test were taken from ibacon's in-house laboratory culture.
The daphnids were bred in the laboratories of ibacon under similar temperature and light conditions as used in the test. The cultivation of the parental daphnids was performed in Elendt M4 medium. The test organisms were not first brood progeny. The daphnids in the stock culture were fed at least on all working days with green algae (Desmodesmus subspicatus) freshly grown in the laboratories of ibacon.
Feeding during the test: none
Acclimatisation: was not necessary, since the test was performed in the same medium as the culturing.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Water Hardness: 2.5 mmol/L (= 250 mg/L) as CaCO3
- Test temperature:
- 18.1 to 19.1 °C at test start;
18.1 to 18.8 °C at test end - pH:
- 7.9 to 8.0 at test start;
7.8 to 8.0 at test end; and thus the pH-value did not vary by more than 1.5 unit - Dissolved oxygen:
- 9.2 to 9.7 mg/L at test start;
11.2 to 12.4 mg/L at test end - Nominal and measured concentrations:
- A filtrate with a loading rate of 100 mg test item/L and dilutions of 1:3.3, 1:10.9, 1:35.9 and 1:118.6, and a control, corresponding to following arithmetic mean measured concentrations of the test item:
4.89, 1.47, 0.456, 0.100 and 0.025 mg test item/L, and a control.
The initial concentrations and the maintenance of the exposure concentrations during the test were verified in the analytical part. All reported results refer to nominal values since the concentrations of the test item were within ± 20% of the nominal concentrations during the test. - Details on test conditions:
- Test Vessel: Glass beakers of 100 mL volume containing approximately 60 mL of test medium. The test vessels were covered with a lid to reduce the loss of water due to evaporation and to avoid the entry of dust into the solutions.
Light Regime: 16 h light : 8 h dark
Light Intensity: The light intensity was 1060 to 1080 lux (measured once during the test). Target: ≤ 1500 lux
Introduction of Daphnids: 20 daphnids per control and test concentration, divided into 4 groups of 5 animals, each group in 60 mL test medium
Replicates: The test was performed with four replicates per treatment group. Exposure Time: 48 hours
Test Procedure: A static test was performed.
Feeding: None
Sampling: Samples were taken at test start (0 hours) and test end (48 hours)
Test Parameter:
Immobility: The mobility of the daphnids was determined by visual observation after 24 and 48 hours. Those animals that are not able to swim within 15 seconds after gentle agitation of the test beaker were considered to be immobile (even if they could still move their antennae).
No pre-experiments were performed. - Reference substance (positive control):
- yes
- Remarks:
- For the evaluation of the quality of the Daphnia clone and the consistency of the experimental conditions, the reference item potassium dichromate is tested at least twice a year to demonstrate satisfactory test conditions.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.53 mg/L
- 95% CI:
- > 1.15 - 4.89
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.32 mg/L
- 95% CI:
- > 0.38 - < 4.61
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.47 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 4.89 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Signs of Intoxication after 48 Hours:
After 48 hours of exposure no immobilisation of the test animals was observed in the control and up to and including the test item concentration of 0.100 mg test item/L. At the concentrations of 0.456 and 1.47 mg test item/L, one animal was immobile. All animals were immobile at the concentration of 4.89 mg test item/L. - Results with reference substance (positive control):
- Results of the most recent Test with the Reference Item Potassium Dichromate in July 2021
Dose response test: 0.40, 0.60, 0.89, 1.33, 2.0 mg/L
EC50 24h: 1.21 mg/L
NOEC 24 h: 0.60 mg/L
LOEC 24 h: 0.89 mg/L
EC50 48 h: 0.844 mg/L
NOEC 48 h: 0.40 mg/L
LOEC 48 h: 0.60 mg/L
The reference test was valid; The results indicate that the sensitivity of the daphnids was consistent with the level proposed by the OECD 202 guideline (EC50-24 h between 0.6 and 2.1 mg potassium dichromate/L). - Reported statistics and error estimates:
- The 24-hour and 48-hour EC50, EC20 and EC10 and the 95 % confidence limits were calculated by weibull analysis.
The NOEC and LOEC after 24 hours were calculated by Fisher´s exact binominal test with Bonferroni corrections and the NOEC and LOEC after 48 hours were calculated by Step-down Cochran Armitage test procedure.
The software used to perform the statistical analysis was ToxRat Professional, Version 3.3.0, ToxRat(R) Solutions GmbH.
Any other information on results incl. tables
Validity criteria
Criterion | value required | value obtained | fullfilled |
Control immobilisation rate | < 10% | 0% | yes |
Dissolved oxygen concentration | at the end of the test >= 3 mg O2/L | ≥ 11.2 mg O2/L in in all treatment groups at the end of the test | yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- see "Any other information on results incl. tables"
- Conclusions:
- The toxic effect of the test item to Daphnia magna was assessed in a static concentration-response test. The 48-hour NOEC was determined to be 1.47 mg/L. The 48-hour LOEC was determined to be 4.89 mg/L and the 48-hour EC50 value was calculated to be 2.53 mg/L.
The initial concentrations and the maintenance of the exposure concentrations during the test were verified in the analytical part. All reported results refer to arithmetic mean concentrations, since the test item concentrations were not within ± 20 % of the nominal concentrations during the test. - Executive summary:
Test Species: Daphnia magna, clone 5; 0.75 to 18.25 hours old
Test Design: This study encompassed 6 treatment groups (5 dose rates of the test item and a control) each containing 20 individuals. The mobility of the daphnids was determined in a static 48-hour test by visual observation after 24 and 48 hours.
Endpoints: Number of immobile organisms after 24 and 48 hours
Test Concentrations: A filtrate with a loading rate of 100 mg test item/L and dilutions of 1:3.3, 1:10.9, 1:35.9 and 1:118.6, and a control, corresponding to following arithmetic mean measured concentrations of the test item:
4.89, 1.47, 0.456, 0.100 and 0.025 mg test item/L, and a control.
Test Conditions: Water temperature: 18.1 to 19.1 °C (target: 18 °C – 22 °C); pH value: 7.8 to 8.0 (target: 6 - 9); dissolved oxygen concentration: 9.2 to 12.4 mg/L (target: ≥ 3 mg/L); photoperiod: 16 h light - 8 h dark; light intensity: 1060 to 1080 lux (target: < 1500 lux); and thus were within the ranges requested by guideline OECD 202
Results: After 48 hours of exposure no immobilisation of the test animals was observed in the control and up to and including the test item concentration of 0.100 mg/L. At the concentrations of 0.456 and 1.47 mg/L, one animal was immobile. All animals were immobile at the concentration of 4.89 mg/L. No sublethal effects on the daphnids were observed in any treatment.
Parameter after 24 h after 48 h EC50 mg test item/L > 4.89 2.53 EC20 mg test item/L > 4.89 1.71 EC10 mg test item/L > 4.89 1.32 NOEC mg test item/L >= 4.89 mg/L 1.47 LOEC mg test item/L > 4.89 4.89 Analytical Results: The quantification of the test item in the test samples was performed using liquid chromatography with UV detection.
The concentrations of the test item were determined in the duplicate test media samples from all test concentrations (test item stock solution and its dilutions) and the duplicate control samples from all sampling times. The mean measured values are
4.89 mg test item/L in the filtrate,
1.47 mg test item/L in the 1:3.3 dilution of filtrate,
0.456 mg test item/L in the 1:10.9 dilution of filtrate,
0.100 mg test item/L in the 1:35.9 dilution of filtrate,
0.025 mg test item/L in the 1:118.6 dilution of filtrate.
Conclusion: The toxic effect of the test item to Daphnia magna was assessed in a static concentration-response test. The 48-hour NOEC was determined to be 1.47 mg/L. The 48-hour LOEC was determined to be 4.89 mg/L and the 48-hour EC50 value was calculated to be 2.53 mg/L.The initial concentrations and the maintenance of the exposure concentrations during the test were verified in the analytical part. All reported results refer to arithmetic mean concentrations, since the test item concentrations were not within ± 20 % of the nominal concentrations during the test.
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