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EC number: 613-906-1 | CAS number: 66212-25-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Feb - Mar 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 17-Hydroxy-1,4,6-pregnatriene-3,20-dione
- EC Number:
- 613-906-1
- Cas Number:
- 66212-25-7
- Molecular formula:
- C21 H26 O3
- IUPAC Name:
- 17-Hydroxy-1,4,6-pregnatriene-3,20-dione
- Test material form:
- solid: bulk
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Kläranlage Berlin Ruhleben
- Method of cultivation: homogenized aerated and stirred - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 10 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Test temperature: 23 - 24 °C
- pH: 7.5 - 7.8
- Continuous darkness: yes
- pH adjusted: yes
TEST SYSTEM
- Number of culture flasks/concentration: 3
- Method used to create aerobic conditions: Aerated with CO2-free air
- Details of trap for CO2 and volatile organics if used: For the determination of the CO2 produced, three CO2 absorber bottles, filled with 100 mL 0.025 N Ba(OH)2 each, were connected in series to the exit air-pipe of each test bottle. The amount of CO2 produced was determined by titration of the remaining Ba(OH)2 with 0.05 N standardized HCI. On days 4, 6, 8, 11, 14, 19, 22, 2'7 and 29 the CO2 absorber bottle nearest to the test bottles was removed for the titration, with the exception of day 29 when all three bottles were removed. The remaining two absorbers were each moved one place closer to the test vessel and a new absorber bottle filled with fresh Ba(OH)2 was placed at the far end of the series. In cases where BaCO3 was also precipitated in the second absorber bottle of any solution, titrations of two Ba(OH)2 bottles were performed. Subsequently, two fresh absorbers were added.
SAMPLING
- Sampling frequency: 4, 6,8, 11, 14, 19, 22, 27, 29 days; After the measurements of pH, 1 mL of concentrated HCl was added to each test vessel in order to convert all dissolved inorganic carbon into CO2.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 3 vessels
- Abiotic sterile control: no
- Toxicity control: yes, 1 vessel
- Reference substance: yes, 1 vessel
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 82
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- The reference substance was completely degraded after 28 d.
Any other information on results incl. tables
Table 1: Amounts of CO2 produced (cumulative), in mg (values for ZK 5668, sodium acetate and toxicity control corrected for blank CO,: production)
Day | ||||||||||
4 | 6 | 8 | 11 | 14 | 19 | 22 | 27 | 29 | ||
Control/blank (mean of 3 vessels) | Concentration of carbon | 2,9 | 5 | 6,50 | 8,6 | 10,6 | 12,7 | 15,5 | 17,7 | 22,6 |
ZK 5668 (mean of 3 vessels) | 0 mg/L | 2,8 | 33 | 49,90 | 64 | 74,6 | 82,6 | 85,3 | 88,3 | 90,2 |
Reference (sodium acetate) | 10 mg/L | 42,5 | 65,7 | 75,00 | 85,5 | 93 | 99,5 | 101 | 103,8 | 105,3 |
Toxicity control (ZK 5668 + sodium acetate) | 10 mg/L + 10 mg/L | 39,8 | 92 | 117,90 | 141,7 | 157,9 | 170,3 | 176,1 | 181,7 | 185,2 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- According to the results of the test it can be concluded that the subsance is readily biodegradable under the conditions of the test.
- Executive summary:
The purpose of this study was to determine the ready biodegradability of 1,4,6-Trienol (ZK 5668). The study was conducted in agreement with the OECD test guideline no. 301 B.
The test substance was incubated in an aqueous solution including nutrients with microorganisms from a municipal sewage treatment plant for 28 days (start of treatment = day 1). The nutrient solutions were made up of phosphates, magnesium sulphate, iron chloride, ammonium chloride and calcium chloide. The test substance was incubated at a concentration of 10 mg carbon/L in triplicate. Additionally, a reference substance (sodium acetate) was tested in a single set according to the same procedure, in order to verify the viability and activity of the degrading microorganisms. One further set was incubated with sodium acetate at 10 mg carbon/L (reference substance) plus test substance at 10 mg carbon/L representing a toxicity control. Furthermore, a blank control was tested in triplicate without any test or refeference substance. The biological degradation of the test and reference substances was evaluated by measurement of the carbon dioxide (CO2) produced during the test period. CO2 production was determined on days 4, 6, 8, 11, 14, 19, 22, 27 and :29. On day 28 the solutions were acidified in order to expel all dissolved CO2 and CO2 was determined on day 29. The CO2 production was calculated as the percentage of total CO2 that the testmaterial could theoretically have produced, based on carbon content. The blank CO2 production was subtracted for correction.
The test compound 1,4,6 -Trienol was degraded to 68% on day 14 and 82% on day 29 (28 days of incubation). The reference compound sodium acetate was degraded to 60% on day 6 and 96% on day 29 (28 days of incubation). In the toxicity control, the reference compound (sodium acetate) plus the test compound, was degraded to 84% on day 29 (28 days of incubation), reflecting the degradation in the individual sets.
Based on the result, the substance is considered being readily biodegradable according to the OECD criteria.
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