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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March 7, 2019 to April 10, 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: HJ/T 153-2004 “The Guidelines for the Testing of Chemical (second version), "No.202 'Daphnia sp. Acute Immobilization Test'"
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Sulfonic Acids, C18-alkane hydroxyl and C18-alkene, potassium salts
- Cas Number:
- 2210238-56-3
- IUPAC Name:
- Sulfonic Acids, C18-alkane hydroxyl and C18-alkene, potassium salts
- Test material form:
- cream / paste
- Remarks:
- Pale yellow paste at 50℃, This sample was obtained from the original liquid form by removing water.
Constituent 1
- Specific details on test material used for the study:
- Storage Condition: Store container tightly closed in well-ventilated place. Store in corrosive resistant/container with a resistant in liner. Store locked up. Don' t store with acids.
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- When the test started (0h) and the test finished (48h), took 1.0 mL every test solution and diluted it with deionized water, then determined the actual concentration of these test solutions by the analytical method for concentration.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The water used for the test was tap-water after aeration and eliminating chlorine. The physicochemical parameters of the water were: pH was 6-9; total hardness was 140 - 250 mg/L CaCO3, the DO was greater than 80% ASV.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The test organisms
The test organisms were Daphnia magna Straus which were obtained from the Institute of Hydrobiology, Chinese Academy of Sciences in April 2018 and it was cultured in the laboratory.
Daphnia Feeding
The tap water which was aerating and eliminating chlorine was put into the 2000 mL beaker. Dozens of Daphnia were added and fed it with cultivated algae of mine lab. The culture temperature was 20 士 2°C; light cycle was 16-hour light and 8-hour dark cycle. Illumination intensity was 1000~1500 Lux. The growth of Daphnia was observed and the young Daphnia were removed out in time every day. The test Daphnia was Daphnia magna which of the age were less than 24h, and the generation was not the fast generation.
Collection of Daphnia
The day before the test started, the young Daphnia were separated from mother Daphnia in incubator. The young Daphnia which of the generation was not the first generation and the age was less than 24h could be obtained on the second day. This Batch of Daphnia was D201903030324.
Study design
- Test type:
- static
- Water media type:
- other: Tap water
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 140~250 mg/L CaCO3
- Test temperature:
- 20±2℃
- pH:
- 6~9
- Dissolved oxygen:
- >80%
- Nominal and measured concentrations:
- Nominal concentrations: 1.82, 2.55, 3.57, 5.00, 7.00, 9.80 mg active ingredient/L
Measured concentrations at 0 h: 2.03, 2.81, 3.84, 5.32, 7.89, 10.63 mg active ingredient/L
Measured concentrations at 48 h: 1.81, 2.44, 3.29, 4.59, 7.01, 9.52 mg active ingredient/L - Details on test conditions:
- Preliminary Test
The nominal concentrations such as 1, 5, 10, 50, 100 mg a.i./L of C18K test solution and blank control were prepared in preliminary test. 50 mL test solution was added to each beaker. Every group added 5 young Daphnia which of the age were less than 24h and the generation was more than the first generation. The static test method was selected to perform preliminary test, the test period was 48h.
The inhibition of Daphnia was observed and recorded at 0h, 24h and 48h. The dissolved oxygen concentration, pH was measured at 0h and 48h. Took a cup of test solution into the incubator which as same as blank group to record the temperature every day. The temperature of the cup of test solution was between 18.9 and 20.3 ℃.
Definitive test
According to test results of the preliminary and method stability test, the nominal concentrations such as 1.82, 2.55, 3.57, 5.00, 7.00, 9.80 mg a.i./L test solution of C18K and blank control were prepared to perform definitive test. The static test method was selected to perform definitive test, and the test period was 48h.
Precisely weighed 0.0512 g test substance C18K and added it into a 100 mL beaker, dissolved it with test water (pH=5.9 土 0.05) and transferred it into a 200 mL volumetric flask and made constant volume to scale with test water, it was ultrasonic dissolved for 10 min and constantly, finally the nominal concentration of 100 mg a.i./L stock solution was obtained.
4.6, 6.4, 8.9, 12.5, 17.5, 24.5 mL stock solution which nominal concentration was 100 mg a.i./L of C18K were added into each 250 mL volumetric flask and made constant volume to scale with test water, then the test solutions which nominal concentrations were 1.82, 2.55, 3.57, 5.00, 7.00, 9.80 mg a.i./L were prepared. Every beaker added 50 mL test solution, four parallel control groups and a blank control group while be set in definitive test.
Every beaker added 5 Daphnia which the age was less than 24h and the generation was more than the first generation. The inhibition of Daphnia was observed and recorded at 0h, 24h and 48h. The dissolved oxygen concentration, pH and the actual concentration of the test solution were measured at 0h and 48h. Took a cup of test solution into the incubator which as same as blank group to record the temperature every day.
Data Analysis
The value of ECx and 95% limit confidence was statisticed with EPA PROBIT ANALYSIS PROGRAM USED FOR CALCULATING LC/EC VALUES VERSION 1.5. - Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.93 mg/L
- 95% CI:
- 6.06 - 8.14
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.27 mg/L
- 95% CI:
- 3.8 - 4.83
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Details on results:
- Preliminary Test
The inhibition of Daphnia was observed and recorded at 0h, 24h and 48h. The dissolved oxygen concentration, pH was measured at 0h and 48h. Took a cup of test solution into the incubator which as same as blank group to record the temperature every day. The temperature of the cup of test solution was between 18.9 and 20.3 ℃. The results of preliminary test were shown below in table 2 and table 3.
Definitive test
The temperature of the cup of test solution was between 19.2 and 20.7 ℃.
The results of definitive test were given in table 4 and table 5.
Test Validity
(1) At the end of the test, the dissolved oxygen of control and test solution were greater than 3.0 mg/L.
(2) At the end of the test, the inhibition rate for Daphnia in the control group is 0, it was not more than I0%.
(3) During the test period, the actual concentration of test solution was greater than 80% of its original measured concentration
(4) The 24h-EC50 of potassium dichromate to Daphnia magna was 1.077 mg/L, the 95% limit confidence was 0.965~1.210 mg/L, the sensitivity of Daphnia magna was compliant with the requirements of guidelines. - Results with reference substance (positive control):
- Sensitivity test
The recent sensitivity test of Daphnia conducted on Feb 19th, 2019. Potassium dichromate was used as the reference substance to carry out the sensitivity test. Reference test result showed that: the Daphnia inhibition ratio was 0 in blank control, and the 24h-EC50 of potassium dichromate on Daphnia was 1.077 mg/L, the 95% limit confidence was 0.965~1.210 mg/L. 24h-EC50 was between 0.6 and 2.1 mg/L, the sensitivity of Daphnia was compliant with the requirements of guidelines.
Any other information on results incl. tables
Table 2 Results of Daphnia Inhibition Quantity in preliminary test
Nominal concentration (mg a.i./L) | 0 | 1 | 5 | 10 | 50 | 100 | |
Quantity of Daphnia | 5 | 5 | 5 | 5 | 5 | 5 | |
Inhibition Quantity | 0h | 0 | 0 | 0 | 0 | 0 | 0 |
24h | 0 | 0 | 2 | 4 | 5 | 5 | |
48h | 0 | 0 | 3 | 5 | 5 | 5 |
Table 3 pH and Dissolved Oxygen result s in preliminary test
Nominal concentration (mg a.i./L) | 0h | 48h | ||
pH | Dissolved Oxygen (mg/L) | pH | Dissolved Oxygen (mg/L) | |
0 | 6.39 | 7.68 | 7.50 | 7.68 |
1 | 6.44 | 7.89 | 7.61 | 7.52 |
5 | 6.47 | 7.94 | 7.56 | 7.39 |
10 | 6.47 | 8.00 | 7.56 | 7.28 |
50 | 6.53 | 8.01 | 7.56 | 7.27 |
100 | 6.61 | 7.78 | 7.58 | 7.26 |
Table 4 Results of Daphnia Inhibition Quantity and Toxicity Symptom in definitive test
Nominal concentration (mg a.i./L) | Quantity of Daphnia | 0h | 24h | 48h | |||
Inhibition Quantity | Toxicity Symptom | Inhibition Quantity | Toxicity Symptom | Inhibition Quantity | Toxicity Symptom | ||
0 | 5 | 0 | A(5) | 0 | A(5) | 0 | A(5) |
5 | 0 | A(5) | 0 | A(5) | 0 | A(5) | |
5 | 0 | A(5) | 0 | A(5) | 0 | A(5) | |
5 | 0 | A(5) | 0 | A(5) | 0 | A(5) | |
1.82 (Actual Value was 1.92) | 5 | 0 | A(5) | 0 | A(5) | 0 | A(5) |
5 | 0 | A(5) | 0 | A(5) | 1 | A(4) E(1) | |
5 | 0 | A(5) | 0 | A(5) | 0 | A(5) | |
5 | 0 | A(5) | 0 | A(5) | 0 | A(5) | |
2.55 (Actual Value was 2.62) | 5 | 0 | A(5) | 0 | A(5) | 0 | A(5) |
5 | 0 | A(5) | 0 | A(5) | 0 | A(3) B(2) | |
5 | 0 | A(5) | 0 | A(5) | 1 | A(4) F(1) | |
5 | 0 | A(5) | 0 | A(0) | 1 | A(4) F(1) | |
3.57 (Actual Value was 3.55) | 5 | 0 | A(5) | 0 | A(5) | 1 | A(4) E(1) |
5 | 0 | A(5) | 1 | A(4) F(1) | 2 | A(3) F(2) | |
5 | 0 | A(5) | 0 | A(5) | 1 | A(4) E(1) | |
5 | 0 | A(5) | 1 | A(4) E(1) | 2 | A(2) B(1) F(2) | |
5.00 (Actual Value was 4.94) | 5 | 0 | A(5) | 0 | A(5) | 3 | B(2) F(3) |
5 | 0 | A(5) | 1 | A(4) F(1) | 4 | B(1) F(4) | |
5 | 0 | A(5) | 1 | A(4) E(1) | 3 | B(2) F(3) | |
5 | 0 | A(5) | 2 | A(3) F(2) | 4 | B(1) F(4) | |
7.00 (Actual Value was 7.44) | 5 | 0 | A(5) | 3 | B(2) F(3) | 5 | F(5) |
5 | 0 | A(5) | 3 | A(2) F(3) | 4 | B(1) F(4) | |
5 | 0 | A(5) | 2 | A(3) F(2) | 4 | B(1) F(4) | |
5 | 0 | A(5) | 2 | A(3) F(2) | 5 | F(5) | |
9.80 (Actual Value was 10.06) | 5 | 0 | A(5) | 5 | F(5) | 5 | F(5) |
5 | 0 | A(5) | 5 | F(5) | 5 | F(5) | |
5 | 0 | A(5) | 3 | B(2) E(1) F(2) | 5 | F(5) | |
5 | 0 | A(5) | 4 | B(1) E(4) | 5 | F(5) | |
EC50 (mg a.i./L) | - | - | 6.93 | 4.27 | |||
95% limit confidence (mg a.i./L) | - | - | 6.06~8.14 | 3.80~4.83 | |||
A-Normal, B-Swimming slowly, C-Anomalous fast swimming, D-Change of body coloration, E-Those animals that are not to swim within 15 seconds after gentle agitating of the test vessel, F-died. |
Table 5 pH and Dissolved Oxygen result s in definitive test
Nominal concentration (mg a.i./L) | Oh | 48h | ||
pH | Dissolved Oxygen (mg/L) | pH | Dissolved Oxygen (mg/L) | |
0 | 6.20 | 7.60 | 7.66 | 7.67 |
1.82 | 6.45 | 7.62 | 7.79 | 7.61 |
2.55 | 6.42 | 7.52 | 7.81 | 7.60 |
3.57 | 6.46 | 7.42 | 7.80 | 7.60 |
5.00 | 6.43 | 7.51 | 7.82 | 7.66 |
7.00 | 6.41 | 7.43 | 7.78 | 7.69 |
9.80 | 6.44 | 7.42 | 7.83 | 7.71 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The result of the definitive test indicated that: The 24h-EC50 of C18K on Daphnia magna in test solution was 6.93 mg a.i./L (actual concentration), 95% limit confidence was 6.06~8.14 mg a.i./L (actual concentration); The 48 h-EC50 of C18K on Daphnia magna in test solution was 4.27 mg a.i./L (actual concentration), 95% limit confidence was 3.80~4.83 mg a.i./L (actual concentration).
- Executive summary:
The testing of the test item was carried out according to the test method "The Guidelines for the Testing of Chemicals" No. 202 'Daphnia sp. Acute Immobilization Test(Second edition)' and "OECD Guidelines for Testing of Chemicals No.202 'Daphnia sp. Acute Immobilization Test"'. According to the study plan and relevant SOP, Daphnia sp. Acute Immobilization Test of C18K was performed.
According to test results of the preliminary and method stability test, the nominal concentrations such as 1.82, 2.55, 3.57, 5.00, 7.00, 9.80 mg a.i./L test solution of C18K and blank control were prepared to perform definitive test. The static test method was selected to perform definitive test, and the test period was 48h. Four parallel control groups and a blank control group while be set in definitive test, 50 mL test solution was added to each beaker, and every beaker added 5 Daphnia which the age was less than 24h and the generation was more than the first generation. The inhibition of Daphnia was observed and recorded at 0h, 24h and 48h. The dissolved oxygen concentration, pH and the actual concentration of the test solution were measured at 0h and 48h. Took a cup of test solution into the incubator which as same as blank group to record the temperature every day. The temperature of the cup of test solution was between 19.3 and 20.7 ℃.
The concentration determination result showed that, in the test period (48h), the test solutions which of the nominal concentration of the test substance C18K were 1.82, 2.55, 3.57, 5.00, 7.00, 9.80 mg a.i./L, the actual concentrations were 1.92, 2.62, 3.55, 4.94, 7.44, 10.06 mg a.i./L.
The result of the definitive test indicated that: The 24h-EC50 of C18K on Daphnia magna in test solution was 6.93 mg a.i./L (actual concentration), 95% limit confidence was 6.06~8.14 mg a.i./L (actual concentration); The 48h-EC50 of C18K on Daphnia magna in test solution was 4.27 mg a.i./L (actual concentration), 95% limit confidence was 3.80~4.83 mg a.i./L (actual concentration) .
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