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EC number: 217-199-7 | CAS number: 1772-25-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2022.08.25-2022.09.19
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- activated sludge
- Details on inoculum:
- For the preparation of the inoculum a sample of aerobic sludge has been selected by the mixed treatment plant of urban (about 66%) and industrial (about 34%) liquid sewage situated at San Rocco - Monza (MB), Italia. The plant of treatment is managed by “BRIANZACQUE SRL (Monza)”.
Activated sludge was collected and used on day of collection. No coarse particles were present so settling was not necessary. Then the mud samples, before their
use, were washed and analyzed to prepare the inoculum. The sludge was continuously aerated at the test temperature. The suspended solids in the mud solution was 3 g/L to have in the final mixture a concentration of 1.5 g/L. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20℃±2
- pH:
- 7.47
- Details on test conditions:
- 24 bottles were prepared for the test as follows:
- 3 different concentrations (1000, 100, 10 mg/L) for the test item at 3 replications/concentrations (9 bottles)
- 3 bottles for the blank control
- 3 different concentrations (50, 10, 1 mg/L) for the test reference (3,5-Dichlorophenol) at 3 replications/concentrations
(9 bottles)
- 3 bottles for abiotic control tested at the highest test item concentration (1 g/L). - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - Blank controls oxygen uptake rate: 22.64 mg oxygen per one gram of activated sludge in an
hour.
- Coefficient of variation of oxygen uptake rate in control replicates: 1.48%
- EC50 of reference substance 3,5 — Dichlorophenol: 8.631 mg/L for total respiration - Results with reference substance (positive control):
- Concentration Reference substance “3,5 - Dichlorophenol”
1 mg/L 11.68%
10 mg/L 50.25%
50 mg/L 86.16% - Validity criteria fulfilled:
- yes
- Conclusions:
- The obtained results, in compliance with assay validity criteria, interpreted in accordance to OECD 209:2010, show that the EC50 value of the test item “HEXANE-1,3,6-TRICARBONITRILE”, after a contact time of 3 hours, results greater than 1000 mg/L.
- Executive summary:
The aim of this study is to determine the effects of the test item “HEXANE-1,3,6-TRICARBONITRILE” on micro-organisms from activated sludge by measuring their respiration rate, according to OECD 209: 2010.
The respiration rates of activated sludge both in control, reference and treatment groups have been measured in an enclosed cell containing an oxygen electrode after a contact time of 3 hours at 20°C±2°C.
This method can be applied to water soluble, poorly water soluble and volatile substances.
The obtained results, in compliance with assay validity criteria, interpreted in accordance to OECD 209: 2010, show that the EC50 value of the test item “HEXANE-1,3,6-TRICARBONITRILE”, after a contact time of 3 hours, results greater than 1000 mg/L.
Reference
Description of key information
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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