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EC number: 953-863-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 2014-08-18 to 2014-09-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was performed in accordance with standard test protocols (OECD 301B resp. EU C.4-C) in a quality controlled laboratory. The study is valid according to criteria mentioned in the test protocols.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Nicotine
- EC Number:
- 200-193-3
- EC Name:
- Nicotine
- Cas Number:
- 54-11-5
- Molecular formula:
- C10H14N2
- IUPAC Name:
- 3-(1-methyl-2-pyrrolidinyl)pyridine
- Test material form:
- liquid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant.
- Pretreatment: The sludge was filtered, washed with tap water twice, then washed with and resuspended in the test medium. It was then aerated until use. The dry matter was determined with 4220 mg suspended solids/litre. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 27 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Stock solutions:
Solution a
Potassium dihydrogen phosphate (KH2PO4) 8.5 g
Di-potassium hydrogen phosphate (K2HPO4) 21.75 g
Di-sodium hydrogen phosphate dihydrate (Na2HPO4*2H2O) 33.4 g
Ammonium chloride (NH4Cl) 0.5 g
H2O demin. ad 1000 mL
The pH was adjusted to 7.4 +/- 0.1.
Solution b
Calcium chloride dihydrate (CaCl2*2H2O) 36.4 g
H2O demin. ad 1000 mL
Solution c
Magnesium sulfate heptahydrate (MgSO4*7H2O) 22.5 g
H2O demin. ad 1000 mL
Solution d
Iron(III) chloride hexahydrate (FeCl3*6H2O) 0.25 g
Di-sodium ethylenediamine tetraacetate dihydrate (Na2EDTA*2H2O) 0.4 g
H2O demin. ad 1000 mL
- Test medium:
Solution a 10 mL
Solution b 1 mL
Solution c 1 mL
Solution d 1 mL
H2O demin. ad 1000 mL
The medium was freshly prepared.
- Test temperature: 21.6 – 23.5 °C
- pH: 7.3 at the end of the test
- Aeration of dilution water: yes
- Continuous darkness: yes
SAMPLING
- Sampling frequency: ten samples were taken within the 28 days
- Sampling method: From each front scrubber flask, ten samples were taken in order to determine the emitted CO2. The sample volume was 1 mL. The resulting change in the volume of the front flask was considered in the calculation of emitted CO2.
On day 28, 5 mL 2 M HCl were added to each test flask in order to drive off dissolved CO2. On day 29, samples from both scrubber flasks were taken.
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2, containing mineral medium and inoculum
- Abiotic sterile control: 1, containing test item, mineral medium and HgCl2
- Toxicity control: 1, containing test item, positive control, mineral medium and in-oculum
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 71
- Sampling time:
- 28 d
- Details on results:
- • The test item nicotine is considered as “readily biodegradable“.
• The degree of biodegradation reached 71% after 28 days.
• The 10-day-window began on day 3, at its end, 72% were reached, surpassing the pass level of 60% given in the OECD guideline.
• Abiotic degradation was not observed.
BOD5 / COD results
- Results with reference substance:
- See table at "Any other information on results incl. tables". Degradation of positive control was >60% within 7 days.
Any other information on results incl. tables
In the following table, the percentage biodegradation is presented
Day |
Positive Control 1 |
Positive Control 2 |
Positive Control Mean |
Test 1 |
Test 2 |
Test Mean |
Abiotic Control |
Toxicity Control |
2 |
0.1 |
2.7 |
1.4 |
0.2 |
0.8 |
0.5 |
-0.1 |
0.1 |
4 |
25.6 |
24.4 |
25.0 |
31.0 |
40.8 |
35.9 |
0.1 |
30.7 |
7 |
69.3 |
73.5 |
71.4 |
64.3 |
66.4 |
65.3 |
-0.5 |
67.5 |
9 |
75.1 |
78.6 |
76.8 |
74.3 |
75.4 |
74.8 |
-1.1 |
74.1 |
11 |
73.9 |
71.5 |
72.7 |
70.7 |
70.2 |
70.4 |
-0.9 |
68.6 |
14 |
72.4 |
77.8 |
75.1 |
73.0 |
73.6 |
73.3 |
-1.3 |
69.5 |
18 |
76.7 |
80.1 |
78.4 |
74.9 |
78.6 |
76.7 |
-1.8 |
80.7 |
23 |
82.9 |
88.2 |
85.6 |
78.4 |
80.3 |
79.3 |
-2.3 |
79.6 |
29 |
72.0 |
80.9 |
76.4 |
70.7 |
70.8 |
70.8 |
-3.9 |
73.1 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- All validity criteria were met. Degradation of the positive control was 71 % after seven days.
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Degradation behaviour of positive control and toxicity control was normal. Abiotic degradation was not observed. Both replicates of the test item showed very good correspondence.
The validity criteria were met and the criterion for ready biodegradability was fulfilled.
If degradation in the toxicity flask is below 25% after 14 days, the test item can be considered as toxic towards the inoculum. As degradation in the toxicity flask was 70% after 14 days, the test item can be stated as “not toxic towards the inoculum in a concentration of 27 mg/L”.
Ready degradability is defined in the guidelines as degradation surpassing 60% within 10 days after reaching a level of 10%. Therefore, the test item nicotine can be considered as “readily biodegradable”.
No observations were made which might cause doubts concerning the validity of the study outcome.
The result of the test can be considered valid.
- Executive summary:
The test item nicotine was tested using a concentration of at least 20 mg organic carbon/L (corresponding to 27.0 mg nicotine/L) in test medium following OECD 301B and EU-Method C.4-C.
Aniline was chosen as positive control.
Activated sludge was used as inoculum (concentration in the test 25.0 mg dry matter/L). The test was left running for 28 days.
All validity criteria were met. Degradation of the positive control was 71% after seven days.
The following data were determined for the test item nicotine:
10-day-window: day 3 – 13
degradation at the end of 10-day-window: 72%degradation at the end of the test: 71%
pass level following guideline: 60% at the end of 10-day-window
Therefore, when applying the 10-day-window, nicotine is readily biodegradable following OECD 301B/EU C.4-C.
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