Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 401-610-3 | CAS number: 122012-52-6 GENIPLEX A
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996-02-05 - 1996-02-22
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- OECD Guidelines for.Testing of Chemicals (1984) No. 209 "Activated Sludge Respiration Inhibition Test"
- Deviations:
- yes
- Remarks:
- Analysis of the concentration, homogeneity and stability of the test materia! in the test preparations was not appropriate to the Test Guideline.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- EEC Commission Directive 87/302/EEC
- Deviations:
- yes
- Remarks:
- Analysis of the concentration, homogeneity and stability of the test materia! in the test preparations was not appropriate to the Test Guideline.
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Amounts of test material (5, 16, 50, 160 and 500 mg) were dispersed together with activated sewage sludge, synthetic sewage and water to a final volume of 500 ml to give the final concentrations of 10, 32, 100, 320 and 1000 mg/l.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected:
A mixed population of activated sewage sludge micro-organisms was obtained on 5 February 1996 from the aeration stage of the Severn Trent Water Pic sewage treatment plant at Belper, Derbyshire, UK which treats predominantly domestic sewage.
- Preparation of inoculum for exposure: Preparation of inoculum
The activated sewage sludge sample was fed 50 ml/I synthetic sewage and maintained on continuous aeration in the laboratory at a temperature of 21 °C. The pH of the sample was 7.5 and the suspended solids equal to 4.0 g/l prior to use. - Test type:
- static
- Water media type:
- freshwater
- Remarks:
- laboratory tap water dechlorinated by passage through an activated carbon filter with a total hardness of approximately 100 mg/l as CaCO3
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- none
- Hardness:
- total hardness of approximately 100 mg/l as CaCO3
- Test temperature:
- 21°C
- Nominal and measured concentrations:
- Definitive study: 10, 32, 100, 320 and 1000 mg/l (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 ml conical flask
- Aeration: yes
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The test water used for both the range-finding and definitive studies was laboratory tap water dechlorinated by passage through an activated carbon filter with a total hardness of approximately 100 mg/l as CaCO3
- Particulate matter:
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Light intensity: The study was conducted under normal laboratory lighting
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : respiratory rate
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.3
- Range finding study
- Test concentrations: nominal test concentrations of 1.0, 10, 100 and 1000 mg/l.
- Results used to determine the conditions for the definitive study: see results - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 180 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - Blank controls oxygen uptake rate:
0.48 - 0.52 mg O2/l/min
- Coefficient of variation of oxygen uptake rate in control replicates: Variation in respiration rates of controls 1 and 2 after 3 hours: ± 4%. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: 3,5-dichlorophenol EC50 (3 hours): 9 mg/l - Validity criteria fulfilled:
- yes
- Conclusions:
- The study was conducted under OECD TG 209, the validity criteria are met and is sufficiently documented to conclude that the study was properly performed and so, the results can be considered reliable. The effect of the test item on the respiration of activated sewage sludge microorganisms gave a 3-hour EC50 of 180 mg/l.
- Executive summary:
Methods: A study was performed to assess the effect of the test material on the respiration of activated sewage sludge. The method followed that described in the OECD Guidelines for.Testing of Chemicals (1984) No. 209 "Activated Sludge Respiration Inhibition Test" and EEC Commission Directive 87/302/EEC under GLP.
Procedure: The test material was aerated for a period of 3 hours at 21° C in the presence of activated sewage sludge with the addition of a synthetic sewage as a respiratory substrate. The rate of respiration was determined after 30 minutes and 3 hours contact time and compared to data for the control and a reference material, 3,5-dichlorophenol.
Results: The effect of the test material on the respiration of activated sewage sludge gave a 3-Hour EC50 of 180 mg/l.
Reference
Description of key information
OECD 209, GLP, activated sewage sludge, respiration inhibition, 3h: 3h EC50 = 180 mg/l
Key value for chemical safety assessment
- EC50 for microorganisms:
- 180 mg/L
- EC10 or NOEC for microorganisms:
- 10 mg/L
Additional information
In the range-finding study, significant inhibition of respiration was observed at 100 and 1000 mg/l, however, there were no significant effects on respiration at 1,0 and 10 mg/l. Based on this information test concentrations of 10, 32, 100, 320 and 1000 mg/l were selected for the main study. Also, this data may indicate a NOEC of 10 mg/L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.