2-(3,5-dimethylhex-3-en-2-yloxy)-2-methylpropyl cyclopropanecarboxylate

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

disregarded due to major methodological deficiencies

Study period:

From 10 September 2008 to 28 October 2008

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Remarks:

The exposure concentrations were too high and therefore the results are not reliaable.

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 B (Bioaccumulation: Semi-static Fish Test)

Deviations:

not specified

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1730 (Fish Bioconcentration Test)

Deviations:

not specified

Qualifier:

according to guideline

Guideline:

other: SEPA. HJ/T 154-2004. The guidelines for the hazard evaluation of new chemical substances. June 2004.

Deviations:

not specified

Qualifier:

according to guideline

Guideline:

other: SEPA. HJ/T 153-2004. The guidelines for the testing of chemicals. June 2004.

Deviations:

not specified

GLP compliance:

yes

Specific details on test material used for the study:

- Storage conditions: Refrigerator 4°C

Batch No. : LDP0800001
Purity : 97%
Date of Expiry : 01 May, 2010

Radiolabelling:

no

Details on sampling:

Sampled water from the test tanks for the determination of test substance concentration before addition of the fish and during the test. Remove seven fish from the test tanks at each sampling time. Rinsed the sampled fish quickly with water, killed instantly, using the most appropriate and humane method, and then weigh.
Sampling days: 0, 3, 7, 10, 14, 21 and 28 days.

Vehicle:

not specified

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

The test adopted zebra fish (Brachydanio rerio), their average body length was 3.0 +/- 0.5 cm (including the length of their tail). Acclimate the stock population of fish for at least 2 weeks in water at the test temperature and feed throughout on a sufficient diet and of the same type to be used during the test.

Route of exposure:

aqueous

Test type:

semi-static

Water / sediment media type:

natural water

Total exposure / uptake duration:

28 d

Hardness:

140 mg CaCO3/L

Test temperature:

no data

pH:

6.5-7.5

Dissolved oxygen:

no data

TOC:

no data

Salinity:

not applicable

Conductivity:

no data

Details on test conditions:

TEST SYSTEM
- Test vessel: The test tank was glass vessel of 25 mL, made 20 L different concentrations solutions in every vessel, then put in 50 fishes.
- No. of vessels per concentration (replicates): 2

TEST MEDIUM / WATER PARAMETERS
The test water was the natural water which had been settled by placing in the air about 24 hours with total hardness about 140 mg CaCO3/L and pH 6.5-7.5.

Nominal and measured concentrations:

5 and 20 mg/L

Reference substance (positive control):

no

Details on estimation of bioconcentration:

The bioconcentration factor at any time during the uptake phase of this bioconcentration test was the concentration of the test substance (Cf, expressed in milligrams per gram) in/on the fish or specified tissues there of, divided by the concentration of the chemical in the surrounding medium (Cw, expressed in milligrams per litre), as BCF = Cf/Cw.

Key result

Conc. / dose:

5 mg/L

Type:

BCF

Value:

0.37 L/kg

Basis:

not specified

Time of plateau:

28 d

Key result

Conc. / dose:

20 mg/L

Type:

BCF

Value:

0.31 L/kg

Basis:

not specified

Time of plateau:

28 d

Details on results:

See table 5.3.1/1 in "Any other information on results incl. tables".

Table 5.3.1/1: Results BCF and concentrations of the test substance in water and fish

Days	5 mg/L			20 mg/L
	Water (mg/L)	Fish (mg/kg)	BCF	Water (mg/L)	Fish (mg/kg)	BCF
0 3 7 10 14 21 28	4.66 4.67 4.58 4.53 4.64 4.57 4.69	ND 2.92 4.88 4.49 3.21 2.47 1.72	- 0.625 1.066 0.991 0.692 0.540 0.366	18.0 18.4 18.8 18.0 18.3 18.7 18.1	ND 11.9 18.7 14.6 13.2 10.4 7.90	- 0.649 0.996 0.813 0.725 0.557 0.313
Mean value			0.713			0.676

Validity criteria fulfilled:

not specified

Conclusions:

The BCF values of the test substance are comprised between 0.313 and 1.07 L/kg.

Executive summary:

The bioconcentration test was performed using zebra fish (Brachydanio rerio) according to OECD Guideline 305B with GLP statement. The test fishes were exposed to saturated concentration of the test substance 5 and 20 mg/L. BCF values were measured following 28 day exposure period.

The purpose of the study was to determine bioconcentration factors (BCF) for fish exposed to a test chemical in aqueous solution. BCF values for the test substance should always be based on concentrations of the chemical in fish tissue and exposure water, and not on total residues. The concentration factor at any time during the uptake phase of this accumulation test was the concentration of the test substance (expressed in milligrams per gram) in/on the fish or specified tissues there of, divided by the concentration of the chemical in the surrounding medium (BCF = Cf/Cw).

The test substance is detected at retention time approximately 7.2 min with GC-MSD. Linear regression equation: A = 295902 c + 297.3 with good linearity of r² = 0.9996 for concentration 1 -20 mg test substance/L. When add standard storage solution of the test substance to test water with concentration between 1 -20 mg/L, the recovery is between 91.1 -91.8% and RSD was 1.15 -1.40%. When add 5 -100 mg/kg in fish with standard storage solution of the test substance, the recovery is between 91.8 -92.6% and RSD was 1.00 -1.07%. The minimum detection amount of MS detector for the test substance is 1.0 x 10-9g and the minimum detection concentration for water and fish sample are 1 mg/L and 1 mg/kg.

When at the nominal concentration 5 mg/L, the mean BCF value of the test substance in fish is 0.714.

When at the nominal concentration 20 mg/L, the mean BCF value of the test substance in fish is 0.676.

These results show that the BCF value of the test substance is 0.313 -1.07. The bioconcentration factor of the test substance in fish is below 10, so the test substance has low bioconcentration.

Description of key information

Invalid study

Additional information

The bioaccumulation experimental study (PEAPC, 2008) was considered as not reliable and was disregarded due to major methodological deficiencies. Indeed, the exposure concentrations were too high and the results are unreliable. The result of this study cannot be considered fit for use.