Ethanol, 2-amino-, reaction products with ammonia, by-products from, distn. Residues

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

February 1992

Reliability:

2 (reliable with restrictions)

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Details on test solutions:

Direct dispersion in water.Test material was dispersed in dechlorinated tap water and the volume adjusted to 1 litre to a give a 4.0 g/l stock solution. Two 500 ml aliquots of this stock solution were each dispersed in dechlorinated tap water and the volume adjusted to 20 litres to give two replicate test concentrations of 100 mg/l.
The test material was known to adsorb to glassware and so saturation of the adsorption sites was achieved by soaking the test vessels overnight prior to the start of the test with the test solutions. At 0 hours the test vessels were emptied, rinsed with the solution to be tested and then refilled with the fresh test solution.
Test media were renewed daily.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Source: Parkwood Trout Farm, Wigmore, Kent, U.K.
Stock held since 14 January 1992 and acclimatised to test conditions from 27 January 1992 - 3 February 1992.
The stock fish were maintained in a glass fibre tank with a "single pass" water renewal system.
The lighting was controlled to give a 16 hours light and 8 hours darkness cycle.
Feeding: Commercial trout pellets daily. Discontinued 24h prior to test.
Mean standard length= 4.5 cm (SO = 0.3 cm).
Mean weight= 0.76 g (SO = 0.11 g).

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

14°C

pH:

7.4 - 9.4

Dissolved oxygen:

10.0 -10.1 mg/L

Nominal and measured concentrations:

Limit test at 100 mg/L plus control

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquaria holding 20 1 of test media.
- Aeration: via naroow bore glass tubes

- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.38 g /L

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16h light:8h dark.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Absence of (i) respiratory movement and (ii) response
to a physical stimulation.

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Conclusions:

The substance cannot be considered harmful to fish.

Description of key information

The 96h LC50 for  fish is >100 mg/l. The substance cannot be considered harmful to fish

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

In a 96 h acute toxicity test according to OECD guidelines, no deaths were observed at the limit concentration 100 mg/L. The substance cannot be considered harmful to fish.