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Diss Factsheets
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EC number: 951-929-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 02/06/2020 - 04/09/2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Batch: 2018060002
pH: 7.60
moisture content: 30.29% - Analytical monitoring:
- yes
- Details on sampling:
- Solutions at T=0h were taken directly from the starting solutions without daphnids. Solutions at T=48h were pooled from two test tubes with daphnids.
- Vehicle:
- no
- Details on test solutions:
- The medium was prepared according to M4 Elendt composition.
Test solutions of required concentrations were prepared by direct addition (by weighing) from the test item with the daphnids medium. The prepared samples were stirred 10 minutes at 20 ± 2°C, darkness. After this step, the samples were filtrated on cellulose acetate filter (porosity: 0.45 µm) after a settling period of 10 minutes. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna Straus, clone number 5, from at least the third generation (i.e. the third brood) obtained with a non-cyclic parthenogenesis reproduction.
The daphnids breeding was carried out in closed bottles placed in a climatic chamber free from any toxic vapour and in medium M4.
Daphnids used in this experimentation were previously filtered in order to obtain animals from a size between 560 µm and 800 µm (corresponding to daphnids less than 24 hours old). - Test type:
- static
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- Observation at 24 and 48 h
- Hardness:
- 260 mg/L of CaCO3
- Test temperature:
- 19.8-20.0°C
- pH:
- 7.5 - 8.1
- Dissolved oxygen:
- 8.4 - 9.1 mg/L
- Nominal and measured concentrations:
- nominal: 0 (control), 10, 18, 32, 58, 104 and 188 mg test item/L
measured (time weighted average): 0 (control), 5.3, 13.7, 22.6, 42.0, 88.3 and 131.0 mg test item/L - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 113 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Remarks:
- Based on total carbon measurement
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 31 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Remarks:
- Based on total carbon measurement
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- The last toxic response obtained on the reference item K2Cr2O7 (May 27, 2020) indicates an EC50-24h of 1.20 mg/L (1.09 – 1.31), indicating that the daphnids sensitivity is correct (within the accepted interval of 0.6–2.1 mg/L fixed by the International Standard ISO 6341 December 2012 as mentioned in the OECD 202 Guideline) and in accordance with historical data obtained by the laboratory
- Validity criteria fulfilled:
- yes
- Conclusions:
- The results obtained for the test item “RICOIL EN” on the toxicological endpoint “immobilisation” give an EC50-48h = 31 mg/L [27 - 36], based on Time-weighted mean measured test item concentration.
These values are based on Time-weighted mean measured test item “RICOIL EN” concentration.
- Executive summary:
The aim of this study was to determine the acute toxicity of the test item “RICOIL EN” on the daphnids Daphnia magna, according to the OECD guideline 202 [7], Limit test 100 mg/L with semi-static-conditions.
Following the results of preliminary tests (non GLP), a final test, at six nominal test item concentration was performed, in order to evaluate the concentration which immobilise X% of the daphnids exposed to the test item after 24 and 48 hours, reported as ECX-24h and ECX-48h, relative to tested concentration.
The immobility of the daphnids was determined in a closed static 48-hours test by visual observation after 24 and 48 hours.
The results obtained for the test item “RICOIL EN” on the toxicological endpoint “immobilisation” give an EC50-48h = 31 mg/L [27 - 36], based on Time-weighted mean measured test item concentration.
These values are based onTime-weighted mean measuredtest item“RICOIL EN”concentration.
Reference
Nominal test item concentration |
Time-weighted mean measured concentations (mg/L) |
Number of daphnids exposed |
Immobilised daphnids (%) |
|
at 24h |
at 48h |
|||
Control |
0 |
20 |
0 (0%) |
0 (0%) |
10 |
5.3 |
20 |
0 (0%) |
0 (0%) |
18 |
13.7 |
20 |
0 (0%) |
0 (0%) |
32 |
22.6 |
20 |
1 (5%) |
3 (15%) |
58 |
42.0 |
20 |
6 (30%) |
17 (85%) |
104 |
88.3 |
20 |
8 (40%) |
20 (100%) |
188 |
131.0 |
20 |
10 (50%) |
20 (100%) |
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 131 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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