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EC number: 250-528-2 | CAS number: 31242-17-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Adsorption / desorption
Administrative data
Link to relevant study record(s)
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 03 April 2019 to 29 May 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
- Qualifier:
- according to guideline
- Guideline:
- other: EC Technical Guidance Document on Risk Assessment Part III Chapter 4.3: Use of (Quantitative) Structure Activity Relationships ((Q)SARs) - Soil and Sediment Sorption
- GLP compliance:
- yes
- Type of method:
- HPLC estimation method
- Media:
- soil
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Lot/batch No.of test material: E01255-297
- Expiration date of the lot/batch: 31 December 2022 - Radiolabelling:
- no
- Details on study design: HPLC method:
- ANALYTICAL CONDITIONS
- Apparatus: Acquity UPLC system (Waters, Milford, MA, USA)
- Type: Acquity UPLC HSS Cyano, 100 mm x 2.1 mm i.d., dp = 1.8 µm (Waters)
- Type, material and dimension of analytical (guard) column:
- Detection system: Acquity UPLC TUV detector (Waters)
- Column temperature: 35 °C ± 1 °C
- Injection volume: 5 µL
- Mobile phase: methanol/water
- Flow: 0.4 mL/min
- UV detection: 210 nm
SOLUTION PREPARATIONS
- Unretained compound: Formamide
- Reference substances: Acetanilide (purity >99.9 %); Monuron (purity 99.9 %); 2,5-Dichloroaniline (purity 99.9 %); Naphthalene (purity 100 %); Benzoic acid phenylester (purity >99.9 %); Fenthion (purity 98.6 %); Phenanthrene (purity 98.5 %); 4,4’-DDT (purity 98.7 %). The log Koc values of the reference substances are based on soil adsorption data reported in OECD 121 guideline.
- Methodology:
A 5 g/L stock solution of formamide (99.2 %) in methanol was used. The stock solution was diluted to obtain an end solution of 55/45 (v/v) methanol/water. The formamide blank solution was 55/45 (v/v) methanol/water.
Stock solutions of the reference substances at concentrations of approximately 1 g/L in methanol or acetonitrile were used. The stock solutions were diluted to obtain an end solution of 55/45 (v/v) methanol/water. The blank solution for the mixture of reference substances was 55/45 (v/v) methanol/water.
A 2000 mg/L stock solution of the test item was prepared in THF. The stock solution was 100-fold diluted with 55/45 (v/v) methanol/water. The final concentration of the test item solution was 20.0 mg/L. A test item blank solution in THF was 100-fold diluted with 55/45 (v/v) methanol/water.
Reference substance and test item solutions were injected in duplicate. Blank solutions were analyzed by single injection. - Key result
- Type:
- log Koc
- Value:
- 2.75 dimensionless
- Temp.:
- 35 °C
- Remarks on result:
- other: 91 % peak area. This peak is a cluster of peaks with a retention time range from 1.28 - 1.86 minutes which corresponds to a log Koc range of 1.99 - 3.31.
- Type:
- log Koc
- Value:
- > 5.63 dimensionless
- Temp.:
- 35 °C
- Remarks on result:
- other: 5.8 % peak area
- Type:
- log Koc
- Value:
- > 5.63 dimensionless
- Temp.:
- 35 °C
- Remarks on result:
- other: 2.9 % peak area
- Details on results (HPLC method):
- In the chromatogram of the test solution, one major peak and two small test item peaks were observed. Detailed results of the HPLC method are given in the table below. The calibration curve of the log k’ of the reference substances as function of log Koc are attached. The equation of the regression line was: log k’ = 0.325 x log Koc – 0.823 (r = 0.98, n = 16).
- Validity criteria fulfilled:
- yes
- Conclusions:
- Using the HPLC method at a neutral pH, soil-adsorption-reference data was applied for the determination of the adsorption coefficient (Koc) of the test material. The log Koc value for the major peak was determined to be 2.75 (this peak is a cluster of peaks with a retention time range from 1.28 - 1.86 minutes, this corresponds with a log Koc range of 1.99 - 3.31).
- Executive summary:
The study was performed based on procedures in the OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC); EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC); EC Technical Guidance Document on Risk Assessment Part III Chapter 4.3: Use of (Quantitative) Structure Activity Relationships ((Q)SARs) - Soil and Sediment Sorption. Using the HPLC method at a neutral pH, soil-adsorption-reference data was applied for the determination of the adsorption coefficient (Koc) of the test material. The log Koc value for the major peak was determined to be 2.75 (this peak is a cluster of peaks with a retention time range from 1.28 - 1.86 minutes, this corresponds with a log Koc range of 1.99 - 3.31).
Reference
Description of key information
The study was performed based on procedures in the OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC); EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC); EC Technical Guidance Document on Risk Assessment Part III Chapter 4.3: Use of (Quantitative) Structure Activity Relationships ((Q)SARs) - Soil and Sediment Sorption. Using the HPLC method at a neutral pH, soil-adsorption-reference data was applied for the determination of the adsorption coefficient (Koc) of the test material. The log Koc value for the major peak was determined to be 2.75 (this peak is a cluster of peaks with a retention time range from 1.28 - 1.86 minutes, this corresponds with a log Koc range of 1.99 - 3.31).
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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