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EC number: 846-153-4 | CAS number: 653592-41-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March 2007 - June 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- adopted 29 December 1992
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- adopted 13 April 2004
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- ethyl 3-[(benzenesulfonyl)oxy]-1-(3-chloropyridin-2-yl)-4,5-dihydro-1H-pyrazole-5-carboxylate
- EC Number:
- 846-153-4
- Cas Number:
- 653592-41-7
- Molecular formula:
- C17H16ClN3O5S
- IUPAC Name:
- ethyl 3-[(benzenesulfonyl)oxy]-1-(3-chloropyridin-2-yl)-4,5-dihydro-1H-pyrazole-5-carboxylate
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: One sample from the freshly prepared test media of the test concentration and the negative and vehicle controls were taken at the start of the test. For the determination of the stability of the test item under the test conditions, one sample from the test concentration and the controls were collected at the end of the test by pouring together the contents of the test beakers of each treatment.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Stock solution: A 100 mg sample of the test item was weighed into a 10 mL volumetric flask and brought to volume with the vehicle acetone (10000 mg/L).
The test solution was prepared by diluting a calculated amount of the stock solution with test medium.
- Controls: Negative control: Test medium; vehicle control: 100 μL acetone was made up to 1 L using test medium.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Age at study initiation: Less than 24 hours
- Source: Local pond at Peenya 2nd stage, Bangalore, INDIA. Bred at Department of Safety Assessment , Advinus Therapeutics Private Limited, Bangalore, INDIA (For ~10 years)
- Feeding during test: No
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Total hardness as CaCO3: 2.41 mmol/L
- Test temperature:
- 20.0 - 21.0ºC
- pH:
- 7.60 - 7.96
- Dissolved oxygen:
- > 82% of air saturation value
- Nominal and measured concentrations:
- Nominal concentration: 1.0 mg/L
Mean measured concentration: 0.71 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL glass beakers
- Fill volume: 150 mL
- Aeration: No
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water prepared according to guideline
- Culture medium different from test medium: no
- Intervals of water quality measurement: Dissolved oxygen concentration and pH were measured at the start and end of the test. The measurements of the test solutions at the start were taken in the absence of Daphnia and the end measurements were made in the presence of Daphnia, after pooling the test solutions from 4 replicates of each group.
OTHER TEST CONDITIONS
- Photoperiod: 16/8 hours light/dark cycle
- Light intensity: 1050-1080 Lux
EFFECT PARAMETERS MEASURED: The immobility of daphnids was determined by visual controls after 24 and 48 hours. The number of immobile daphnids in each group was recorded.
VEHICLE CONTROL PERFORMED: yes
RANGE-FINDING STUDY
- Test concentrations: 0.01, 0.1 and 1.0 mg/L
- Results used to determine the conditions for the definitive study: After 48 hours test duration in the controls (negative and vehicle) and the test concentrations of 0.01, 0.1 and 1.0 mg/L no immobilization was determined. The definitive study was therefore conducted as a limit test at the solubility of the test item, 1.0 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (0.32 mg/L)
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.71 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Details on results:
- The exposure of daphnids to the mean measured concentration of 0.71 mg/L resulted in no immobilization at the end of 48 hours. Also, no immobility was observed in the negative or the vehicle control. The observed NOEC, LOEC and EC50 values were greater than 0.71 mg/L based on the mean measured concentration.
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
There was 30 and 80 percent immobilization of Daphnia in the positive control group at 24 and 48 hours after test initiation, respectively, demonstrating the sensitivity of the test procedure. These values are typical for this positive control.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- No immobilization was observed in the negative as well as vehicle control at the end of the test. The dissolved oxygen concentration of the test medium was greater than 82% of the air saturation value.
- Conclusions:
- The acute toxicity of the test item to Daphnia magna was assessed in a static limit-test. The 48-hour NOEC was determined to be at least 0.71 mg/L (mean measured concentration). The 48‑hour LOEC and the 48-hour EC50 were > 0.71 mg/L (mean measured concentation).
- Executive summary:
The acute toxicity of the test item to Daphnia magna was determined in a static 48-hour immobilisation limit test. The test was conducted in accordance with OECD Guideline 202.
The daphnids were exposed to at a nominal concentration of 1 mg/L test item over a 48-hour period. A concurrent negative control (dilution water only), a vehicle control (acetone) and a positive control (potassium dichromate) were also tested. HPLC analysis of the test solutions showed reduction in the measured concentration of the test item in the test media at the end of the treatment period compared to the nominal concentration. Hence, the results are reported based on the mean measured concentration of the test item.
The test solutions were observed for immobilisation of Daphnia at 24 and 48 hours after test initiation. The exposure of daphnids to the mean measured concentration of 0.71 mg/L resulted in no immobilization at the end of 48 hours. Also, no immobility was observed in the negative as well as vehicle control. The observed NOEC, LOEC and EC50 values were greater than 0.71 mg/L based on the mean measured concentration.
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