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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- 2010
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Batch number: TROD7BB06
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The sludge used was collected at the domestic sewage treatment plant of Piracicaba, Águas do Mirante. It was collected and used on the same day of the test.
pH of sludge: 7.01
dry matter of sludge: 3.0 g suspended solids/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2°C
- pH:
- 7.6-8.0
- Nominal and measured concentrations:
- nominal: 0, 10, 100 and 1000 mg/L.
- Details on test conditions:
- Duration Three hours
Replicates One replicate/treatment (positive control)
One replicate for concentration (10 mg L-1)
One replicate for concentration (100 mg L-1)
Three replicates for concentration (1000 mg L-1)
Water Tap water
Feeding Nutrient solution, 16 mL vessel-1
Temperature 20 ± 2ºC
The study was performed in a closed system with aeration. In the control vessels, 16 mL of nutrient solution was mixed with 234 mL of water. The positive control and the test vessels were prepared by adding the appropriate amount of positive control solution respectively test item into the test vessel, adding 16 mL nutrient solution and water to give 250 mL. Then, 250 mL inoculum was added in ten minutes intervals. After addition of the inoculums, the flasks were closed immediately and the mixtures were stirred with aeration.
After three hours the contents were poured into a tube of approximately 50 mL and the respiration rate was determined by measuring the O2 concentration over a ten minutes period. The following vials were measured equally at ten minutes intervals. - Reference substance (positive control):
- yes
- Remarks:
- For the positive control, copper sulfate pent hydrate was used in three concentrations (58 mg L-1, 100 mg L-1 and 180 mg L-1). The substance was weighed directly into the bottles (29-50-90 mg for 500 mL total volume)
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: oxygen consumption
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: oxygen consumption
- Details on results:
- O2-Consumption, Inhibition - test substance.
Test Concentration pH Consumption (mg L-1 h) Inhibition (%) Variation in DO consumption of controls (%)
Control C1 8.00 37.24 -
F1 – 1% 8.00 47.31 0.00
F2 – 10% 7.78 38.57 0.00
F3 – 100% 7.75 42.94 0.00
F3 – 100% 8.00 43.09 0.00
F3 – 100% 7.93 43.05 0.00
Abiotic control 7.06 0.00 -
Control C2 7.57 39.90 - - Validity criteria fulfilled:
- yes
- Conclusions:
- A preliminary test was conducted to evaluate the inhibition of oxygen consumption. Three concentrations of the test substance (10, 100 and 1000 mg/L) were used, and the third concentration was done in triplicate. The sludge used had a concentration of 3.0 g/L solids.
As there was an absence of inhibition of oxygen consumption by the test substance, the definitive test becomes unnecessary. - Executive summary:
The effects on microorganisms from activated sludge were determined by measuring their respiration rate under defined conditions in the presence of different concentrations of the test substance. The respiration rates of samples of activated sludge fed with synthetic sewage were measured in an enclosed cell containing an oxygen electrode after a contact time of three hours.
Three concentrations of the test substance (10, 100 and 1000 mg/L) were used, and the third concentration was done in triplicate. The sludge used had a concentration of 3.0 g/L solids. There was an no inhibition of oxygen consumption by the test substance.
Reference
Description of key information
The effects on microorganisms from activated sludge were determined by measuring their respiration rate under defined conditions in the presence of different concentrations of the test substance. The respiration rates of samples of activated sludge fed with synthetic sewage were measured in an enclosed cell containing an oxygen electrode after a contact time of three hours.
Three concentrations of the test substance (10, 100 and 1000 mg/L) were used, and the third concentration was done in triplicate. The sludge used had a concentration of 3.0 g/L solids. There was an no inhibition of oxygen consumption by the test substance.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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