4-propylcyclohexanone

Endpoint summary

Description of key information

Short-term toxicity to fish:

The acute toxicity test with Zebrafish was performed according to OECD 203. Under the given conditions the 96h LC50 value was 43 mg/L (reference 6.1.1-1).

Short-term toxicity to aquatic invertebrates:

An acute immobilization study with Daphnia magna was performed according to OECD 202. Under the given conditions the 48-h EC50 value was 33 mg/L(reference 6.1.3-1).

Toxicity to microorganisms:

The toxic effects of the test substance on the oxygen consumption rate of aerobic microorganisms (activated sludge) after 30 min was determined according to the OECD 209. The EC50 was determined to be 806 mg/L (reference 6.1.7-1).

Additional information

Short-term toxicity to fish:

An investigation of the lethal effects of test substance to the Zebra-fish (Brachydanio rerio) was carried out according to OECD TG 203. Fish were exposed in separate test batches to different concentrations of test substance in the test medium over a test period of 96 h (each tank with 10 fish). The concentrations used in the test were 0, 1, 10, 18, 32, 58 and 100 mg/L. Criteria for the inspections of test fish during the exposure period were, above all, the mortality rates, but moreover abnormal symptoms in the swimming behaviour, in visible physiological functions as well as in the outward appearance of the test fish were recorded. Fish were considered dead if touching of the caudal peduncle produced no reaction and if no breathing movements could be observed. Under the conditions of this study, the 96-h LC50 and NOEC were determined to be 43 mg/L and 18 mg/L, respectively (reference 6.1.1-1).

Short-term toxicity to aquatic invertebrates:

The acute toxicity of the test material was determined in a study according to OECD TG 202. For this purpose, young Daphnia magna were exposed to aqueous test material concentrations under defined conditions. The study comprised of one vessels per concentration containing 20 Daphnia magna, i.e. 20 Daphnia per concentration (test medium group) and control group. The Daphnia were observed for  immobilization/deaths up to 48 hours. Daphnia magna were exposed to the nominal test material concentrations of 1, 10, 18, 32, 58 and 100 mg/L in a static system. The nominal concentrations of 18 to 100 mg/L led to immobilized daphnia. Under the conditions of this study, the 48-h EC50 was determined to be 33 mg/L (reference 6.1.3-1).

Toxicity to microorganisms:

The influence of the test item on the activity of activated sludge was evaluated by measuring the respiration rate under defined conditions according to the OECD TG 209. A defined amount of activated sludge is mixed with synthetic medium and the respiratory rate is measured. This rate of respiration is compared to that measured in test mixtures with different concentrations of the test substance. The test substance was tested at the following concentrations: 100, 180, 320, 560 and 1000 mg/L. The sensitivity of the activated sludge used is checked with 3,5-dichlorophenol as reference substance. In this Activated Sludge, Respiration Inhibition Test an EC50 after 30 min of 806 mg/L was determined (reference 6.1.7-1).