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EC number: 241-304-5 | CAS number: 17268-47-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018-09-21 to 2018-10-09
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Principles of method if other than guideline:
- not applicable
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control, 10, 18, 32, 56 and 100 mg/L
- Sampling method: Samples were taken from the control and each test group from the bulk test preparation at 0 hours and from the pooled replicates at 72 hours for quantitative analysis. set of duplicate samples was taken at 0 and 72 hours and stored frozen for further analysis if necessary.
- Sample storage conditions before analysis: All samples were stored frozen prior to analysi - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A nominal amount of test item (200 mg) was dissolved in culture medium and the volume adjusted to 2 liters to give a 100 mg/L stock solution from which a series of dilutions was made to give further stock solutions of 56, 32, 18 and 10 mg/L. The stock solutions and each of the prepared concentrations were inverted several times to ensure adequate mixing and homogeneity. The concentration and stability of the test item in the test preparations were verified by chemical analysis at 0 and 72 hours
- Controls: negative control
- All other template details: Not reported - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: CCAP 278/4
- Source (laboratory, culture collection): Culture Collection of Algae and Protozoa (CCAP), SAMS Research Services Ltd, Scottish Marine Institute, Oban, Argyll, Scotland
- Age of inoculum (at test initiation): not reported
- Method of cultivation: not reported
- All other template details: Not reported - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- Not reported
- Hardness:
- Not reported
- Test temperature:
- 24 ±1 ºC
- pH:
- 7.9 to 8.9 (control cultures)
A concentration dependent increase in pH was observed in the test preparations at 0 hours in the range of pH 8.8 at 10 mg/L through to pH 9.8 at 100 mg/L which was considered to be due to an intrinsic property of the test item - Dissolved oxygen:
- Not reported
- Salinity:
- Not reported
- Conductivity:
- Not reported
- Nominal and measured concentrations:
- Nominal test concentrations (mg/L): 10, 18, 32, 56 and 100.
Analysis of the test preparations at 0 and 72 hours showed measured test concentrations to range from 84% to 109% of nominal and so the results are based on nominal test concentrations only - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL glass conical flasks
- Material, size, headspace, fill volume: 250 mL glass conical flasks each containing 100 mL of test preparation and plugged with polyurethane foam bungs to reduce evaporation
- Renewal rate of test solution (frequency/flow rate):
- Initial cells density: 5.0E+3 cells/mL for control
- Control end cells density: 1.82E+06 cells/mL for control.
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): not applicable
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH was measured at the start and after 72 hours
- All other template details: Not reported
OTHER TEST CONDITIONS
- Sterile test conditions: not reported
- Adjustment of pH: no.
- Photoperiod: continuous
- Light intensity and quality: approximately 7000 lux
- Salinity (for marine algae): not applicable
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: coulter counter after 25, 48, 72 hours
- Chlorophyll measurement: not reported
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: range finding test: 0.10, 1.0, 10 and 100 mg/L.
- Results used to determine the conditions for the definitive study: The results of the range-finding test showed no effect on growth at the test concentrations of 0.10, 1.0 and 10 mg/L. However, growth was observed to be reduced at 100 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 67 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 78 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 26 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 14 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no abnormalities after 72h
- Colour differences: At the start of the test all control and test cultures were observed to be clear colorless solutions. After the 72-Hour test period all control, 10, 18 and 32 mg/L test cultures were green dispersions whilst the 56 and 100 mg/L test cultures were light green dispersions.
- All other template details: Not reported - Results with reference substance (positive control):
- ErC50 (0 to 72 hour) : 1.6 mg/L; 95% confidence limits 1.4 to 1.8 mg/L
EyC50 (0 to 72 hour) : 0.77 mg/L; 95% confidence limits 0.68 to 0.87 mg/L
NOEC growth rate: 0.25 mg/L
NOEC yield: 0.25 mg/L
LOEC growth rate: 0.50 mg/L
LOEC yield: 0.50 mg/L - Reported statistics and error estimates:
- Not reported
- Validity criteria fulfilled:
- yes
- Remarks:
- Cell concentration of the control cultures increased by a factor of 364 after 72 h. The mean coefficient of variation for section by section specific growth rate for the control cultures was 9%.
- Conclusions:
- There were no statistically significant differences (P=0.05), between the control, 10 and 18 mg/L test concentrations, however all other test concentrations were significantly different (P<0.05) and, therefore the NOEC based on growth rate was 18 mg/L. Correspondingly the LOEC based on growth rate was 32 mg/L.
There were no statistically significant differences (P=0.05), between the control, 10 and 18 mg/L test concentrations however all other test concentrations were significantly different (P<0.05) and, therefore the NOEC based on yield was 18 mg/L. Correspondingly the LOEC based on yield was 32 mg/L. - Executive summary:
An algal growth inhibition tests for the test was performed accroding to OECD 201. Pseudokirchneriella subcapitata was exposed to an aqueous solution of the test item at concentrations of 10, 18, 32, 56 and 100 mg/L (three replicate flasks per concentration) for 72 hours, under constant illumination and shaking at a temperature of 24 ±1 °C. Analysis of the test preparations at 0 and 72 hours showed measured test concentrations to range from 84% to 109% of nominal and so the results are based on nominal test concentrations only.
Reference
Exposure of Pseudokirchneriella subcapitata to the test item gave the following results:
Response variable | EC50 (mg/L) | NOEC (mg/L) | LOEC (mg/L) |
Growth Rate | >100* | 18 | 32 |
Yield | 78 | 18 | 32 |
* It was not possible to calculate an ErC50 value as no more than 15% inhibition of growth rate occurred at the maximum concentration employed
Description of key information
An algal growth inhibition tests for the test was performed accroding to OECD 201. Pseudokirchneriella subcapitata was exposed to an aqueous solution of the test item at concentrations of 10, 18, 32, 56 and 100 mg/L (three replicate flasks per concentration) for 72 hours, under constant illumination and shaking at a temperature of 24 ±1 °C. Analysis of the test preparations at 0 and 72 hours showed measured test concentrations to range from 84% to 109% of nominal and so the results are based on nominal test concentrations only.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 18 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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