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EC number: 816-845-0 | CAS number: 1818326-42-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From January 25, 2018 to April 24, 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- GLP
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- May 30, 2008
- Deviations:
- yes
- Remarks:
- deviations from the recommended temperature (during < 2 hours). No impact suspected on the study.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- Adopted July 17, 1992.
- Deviations:
- yes
- Remarks:
- deviations from the recommended temperature (during < 2 hours). No impact suspected on the study.
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Aerobic activated sludge, microorganisms from a domestic waste water treatment plant was supplied by the sewage treatment plant Rossdorf, Germany
- Pretreatment : 3 times, the activated sludge was washed by centrifugation and the supernatant liquid phase was decanted after 20 minutes of deposition. The solid material was re-suspended in test water and centrifuged again.
- Preparation of inoculum for exposure: An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 3.5 g dry material per litre were mixed with test water
- Concentration of sludge: 3.5 g dry material per litre
- Storage conditions : The final suspension was aerated overnight before its use in the experiment. - Duration of test (contact time):
- <= 28 d
- Initial conc.:
- 103.1 mg/L
- Based on:
- test mat.
- Initial conc.:
- 279.8 mg/L
- Based on:
- ThOD
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Analytical grade salts were added to pure water to prepare the following stock solutions:
a) 8.5 g KH2PO4, 21.75 g K2HPO4, 33.4 g Na2HPO4 x 2 H2O, 0.5 g NH4Cl filled up with pure water to 1000 mL volume; The pH-value was 7.4.
b) 11.25 g MgSO4 x 7 H2O filled up with pure water to 500 mL volume
c) 18.2 g CaCl2 x 2 H2O filled up with pure water to 500 mL volume
d) 0.125 g FeCl3 x 6 H2O filled up with pure water to 500 mL volume
In order to avoid precipitation of iron hydroxide in the stock solution d), one drop of concentrated HCl per litre was added before storage.
50 mL of stock solution a) and 5 mL of the stock solutions b) to d) were combined and filled up to a final volume of 5000 mL with pure water. The pH of the test water was set to 7.4 from 6.4 with Sodium hydroxide
- Test temperature: 22°C ± 1°C
- pH: 7.4 ± 0.2
- pH adjusted : yes
- CEC (meq/100 g): not reported
- Aeration of dilution water : continuous stirring
- Suspended solids concentration : 3.5 g/L sludge stock suspension and final concentration in the tested flasks is 28.7 mg sludge/L
- Continuous darkness: yes
The amounts of test item and reference item were directly weighed into the test flasks.
TEST SYSTEM
- Culturing apparatus: Manometric Test System with test flasks containing a volume of approximately 500 mL.
- Number of culture flasks/concentration: 2 test flasks
- Method used to create aerobic conditions: not reported
- Measuring equipment: BSB/BOD-Sensor-System, Aqualytic Dortmund, Germany
- Details of trap for CO2 : Potassium hydroxide solution (45%) was used for trapping the produced carbon dioxide.
SAMPLING
- Sampling method: daily measurements of pressure decrease
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 flasks
- test flask : 2 flasks
- Inoculum reference : 1 flask
- Abiotic sterile control: 1 flask
- Toxicity control: 1 flask
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- tested at the concentration of 102 mg/L, corresponding to 170 mg/L ThOD(NH4)
- Test performance:
- Climatised chamber : Temperature: 22°C ± 1°C
7.4 to 7.5 (measured at the start of the test)
7.2 to 7.6 (measured at the end of the test) - Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 68
- Sampling time:
- 28 d
- Remarks on result:
- other: The tested substance is considered readily biodegradable because it is an UVCB and > 60% biodegradation is reached in 28 days, although failing the 10 day window.
- Details on results:
- In the toxicity control containing both, the test item and the reference item sodium benzoate, 48% (ThODNH4) biodegradation was noted within 14 days and 60% (ThODNH4) biodegradation after 28 days of incubation. The test item is not considered as inhibitory to the aerobic activated sludge microorganisms because degradation was >25% within 14 days.
- Results with reference substance:
- The reference item sodium benzoate was sufficiently degraded to 79% after 14 days and to 84% after 28 days of incubation, thus confirming the suitability of the aerobic activated sludge inoculum used.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable, but failing 10-day window
- Remarks:
- Since the substance is an UVCB and it reach > 60% in 28 days, it is considered as readily bioderadable.
- Conclusions:
- The tested substance "LAB 4448" demonstrated 68% biodegradation in OECD 301F test and it is considered as readily biodegradable.
- Executive summary:
The substance LAB 4448 was tested for its ready biodegradation according the EU Method C.4 -D and OECD Guideline 301F, and in compliance with the GLP.
The biodegradation was followed through the oxygen consumption measured by the pressure changes in the tested flasks, and the CO2 emitted is absorbed by a solution of potassium hydroxyde. The inoculum is prepared from activated sludge coming from a domestic wastewater treatment plant. The concentration of the inoculum is 28.7 mg sludge per liter. The substance LAB 4448 is tested in two replicates at the concentration of 103.1 mg/L, corresponding to 279.8 mg/L (ThOD(NH4)). Two replicates of blank control containing only the inoculum, one flask of the reference control, and one flask of the toxicity control (with the tested and the reference substances) were also tested in parallel.
All the validity criteria were fullfilled. The reference substance, sodium benzoate, was sufficiently degraded to 79% after 14 days, thus confirming the suitability of the aerobic activated sludge inoculum. No toxicity was observed in the toxicity control.
The mean biodegradation of LAB 4448 at the end of the test was 68% (ThODNH4). The 10-Day window criteria was not passed. However since the LAB 4448 substance is an UVCB with carbon chains of different lengths, this criteria has not to be fullfilled and LAB 4448 is considered as readily biodegradable.
This biodegradation study is acceptable, and satisfies the requirements of the Method C.4-D and OECD Guideline 301F.
Reference
Cumulative Biochemical Oxygen Demand (mg O2/L) in Test Flasks during the Test Period of 28 Days
Time |
Flask No. |
|||||||
(days) |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
|
1 |
5 |
20 |
5 |
5 |
55 |
0 |
75 |
|
2 |
15 |
45 |
5 |
5 |
95 |
0 |
105 |
|
3 |
30 |
55 |
10 |
10 |
100 |
0 |
120 |
|
4 |
50 |
70 |
10 |
10 |
115 |
0 |
140 |
|
5 |
70 |
85 |
10 |
15 |
125 |
0 |
160 |
|
6 |
85 |
95 |
15 |
15 |
130 |
0 |
170 |
|
7 |
100 |
105 |
15 |
15 |
135 |
0 |
180 |
|
8 |
110 |
115 |
15 |
15 |
140 |
0 |
190 |
|
9 |
120 |
120 |
15 |
15 |
145 |
0 |
195 |
|
10 |
130 |
125 |
15 |
15 |
145 |
5 |
205 |
|
11 |
135 |
135 |
15 |
15 |
150 |
10 |
210 |
|
12 |
145 |
135 |
15 |
15 |
150 |
20 |
215 |
|
13 |
150 |
140 |
15 |
15 |
150 |
35 |
220 |
|
14 |
160 |
145 |
15 |
15 |
150 |
45 |
230 |
|
15 |
165 |
150 |
15 |
15 |
155 |
50 |
235 |
|
16 |
170 |
155 |
15 |
15 |
155 |
55 |
240 |
|
17 |
175 |
160 |
15 |
15 |
155 |
60 |
245 |
|
18 |
180 |
165 |
15 |
15 |
155 |
60 |
255 |
|
19 |
185 |
165 |
15 |
15 |
160 |
65 |
265 |
|
20 |
190 |
170 |
15 |
15 |
160 |
70 |
270 |
|
21 |
195 |
170 |
15 |
15 |
160 |
70 |
275 |
|
22 |
200 |
175 |
15 |
20 |
160 |
70 |
280 |
|
23 |
205 |
175 |
15 |
20 |
160 |
75 |
280 |
|
24 |
210 |
180 |
15 |
20 |
160 |
75 |
280 |
|
25 |
210 |
180 |
15 |
20 |
160 |
80 |
280 |
|
26 |
215 |
185 |
15 |
20 |
160 |
80 |
285 |
|
27 |
220 |
185 |
15 |
20 |
160 |
85 |
285 |
|
28 |
225 |
190 |
15 |
20 |
160 |
85 |
285 |
|
Flasks 1 and 2: LAB 4448
Flasks 3 and 4: inoculum control
Flask5: reference(procedure control)
Flask 6: abiotic control
Flask 7: toxicity control
Percentage Biodegradation of Test Item, of Sodium Benzoate and of the Toxicity Control
based on ThODNH4
Time |
Percentage Biodegradation1 |
|||
(Days) |
LAB 44481 |
Sodium Benzoate2 |
Toxicity |
|
|
Flask 1 [%] |
Flask 2 [%] |
Flask 5 [%] |
Flask 7 [%] |
1 |
0 |
5 |
29 |
16 |
2 |
4 |
14 |
53 |
22 |
3 |
7 |
16 |
53 |
25 |
4 |
14 |
21 |
62 |
29 |
5 |
21 |
26 |
66 |
33 |
6 |
25 |
29 |
68 |
35 |
7 |
30 |
32 |
71 |
37 |
8 |
34 |
36 |
74 |
39 |
9 |
37 |
38 |
76 |
40 |
10 |
41 |
39 |
76 |
43 |
11 |
43 |
43 |
79 |
44 |
12 |
46 |
43 |
79 |
45 |
13 |
48 |
45 |
79 |
46 |
14 |
52 |
47 |
79 |
48 |
15 |
53 |
48 |
82 |
49 |
16 |
55 |
50 |
82 |
50 |
17 |
57 |
52 |
82 |
52 |
18 |
59 |
54 |
82 |
54 |
19 |
61 |
54 |
85 |
56 |
20 |
62 |
55 |
85 |
57 |
21 |
64 |
55 |
85 |
58 |
22 |
65 |
56 |
84 |
59 |
23 |
67 |
56 |
84 |
59 |
24 |
69 |
58 |
84 |
59 |
25 |
69 |
58 |
84 |
59 |
26 |
70 |
60 |
84 |
60 |
27 |
72 |
60 |
84 |
60 |
28 |
74 |
62 |
84 |
60 |
1ThODNH4of
LAB 4448: 2.715 mg O2/mg test item
2ThODNH4of
sodium benzoate: 1.666 mg O2/mg reference item
Description of key information
The tested substance "LAB 4448" demonstrated 68% biodegradation in OECD 301F test and it is considered as readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
The substance LAB 4448 was tested for its ready biodegradation according the EU Method C.4 -D and OECD Guideline 301F, and in compliance with the GLP.
The biodegradation was followed through the oxygen consumption measured by the pressure changes in the tested flasks, and the CO2 emitted is absorbed by a solution of potassium hydroxyde. The inoculum is prepared from activated sludge coming from a domestic wastewater treatment plant. The concentration of the inoculum is 28.7 mg sludge per liter. The substance LAB 4448 is tested in two replicates at the concentration of 103.1 mg/L, corresponding to 279.8 mg/L (ThOD(NH4)). Two replicates of blank control containing only the inoculum, one flask of the reference control, and one flask of the toxicity control (with the tested and the reference substances) were also tested in parallel.
All the validity criteria were fullfilled. The reference substance, sodium benzoate, was sufficiently degraded to 79% after 14 days, thus confirming the suitability of the aerobic activated sludge inoculum. No toxicity was observed in the toxicity control.
The mean biodegradation of LAB 4448 at the end of the test was 68% (ThODNH4). The 10-Day window criteria was not passed. However since the LAB 4448 substance is an UVCB with carbon chains of different lengths, this criteria has not to be fullfilled and LAB 4448 is considered as readily biodegradable.
[Type of water: freshwater]
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