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EC number: 700-251-2 | CAS number: 72684-95-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Acute oral and acute dermal toxicity of 4-hydroxy-3,5-dimethoxybenzonitrile were examined in rats.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-12-12 to 2009-01-23
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.1100 (Acute Oral Toxicity)
- Deviations:
- yes
- Remarks:
- Dose level selection changed to limit test at 5000 mg/kg
- GLP compliance:
- yes
- Test type:
- up-and-down procedure
- Limit test:
- yes
- Specific details on test material used for the study:
- - Composition of test material, percentage of components: syringonitrile 99.8%
- Expiration date of the lot/batch: May 2010
-Physical description: pale yellow powder
-solubility: soluble in water
-stability: test substance was expected to be stable for the duration of the testing. - Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Received from Ace Animals Inc, Boyertown, PA, on Nov. 25 and Dec. 30, 2008
- Age at study initiation: young adult (9-11 weeks)
-Weight at study initiation: 178-220 grams
- Housing: singly housed in suspended stainless steel cage
- Diet (e.g. ad libitum): pelleted Purina Rodent Chow
- Water (e.g. ad libitum): filtered tap water was supplied ad libitum by an automatic water dispensing system.
- Acclimation period:10-20 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-21°C
- Humidity (%): 15-60%
- Photoperiod (hrs dark / hrs light):12 hr light/dark cycle - Route of administration:
- oral: gavage
- Vehicle:
- CMC (carboxymethyl cellulose)
- Details on oral exposure:
- - Concentration in vehicle: Test substance was administered as a 35% w/w mixture in a 0.5% solution of carboxymethyl cellulose in distilled water using a stainless steel ball-tipped gavage needle attached to an appropriate syringe.
- Doses:
- Limit test at 5000 mg/kg
- No. of animals per sex per dose:
- 3 female rats
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: observed for gross toxicity, mortality and behavioural changes during the first several hours after dosing and at least once daily thereafter for 14 days. Weights recorded pre-dosing, day 7 and day 14.
- Necropsy of survivors performed: yes -- tissues and organs of thoracic and abdominal cavities were examined. - Preliminary study:
- No mortality was recorded in this study. There were no overt signs of systemic toxicity throughout the 14-day observation period and at necropsy. There were no adverse effects on body weights and body weight gains. No gross abnormalities were noted at necropsy.
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 5 000 mg/kg bw
- Mortality:
- All animals survived.
- Clinical signs:
- other: Following administration, two out of three rats were hypoactive/exhibited piloerection, hunched posture and reduced faecal volume. However, the animals recovered by day 2, appeared active and healthy for the remainder of 14-day observation period.
- Gross pathology:
- No gross abnormalities observed when necropsied at the end of 14-day study.
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The acute oral LD50 of the test substance is greater than 5000 mg/kg of body weight in female rats.
- Executive summary:
The objective of this study was to assess the acute toxicity of Mediator SNP (4-hydroxy-3,5-dimethoxybenzonitrile) when administered as a single oral dose followed by a 14-day period of observation. The information is used for both hazard assessment and ranking purposes. The study was initiated with an initial limit dose of 5000 mg/kg body weight in three female rats. The test substance was administered as a 35% w/w mixture in a 0.5% solution of carboxymethylcellulose (CMC) in distilled water.
No mortality was recorded in this study. There were no overt signs of systemic toxicity throughout the 14-day observation period and at necropsy. There were no adverse effects on body weights and body weight gains. No gross abnormalities were noted at necropsy. Under the conditions of this study, the oral LD50 was >5000 mg/kg body weight.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 5 000 mg/kg bw
Acute toxicity: via inhalation route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-12-04 to 2008-12/18
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.1200 (Acute Dermal Toxicity)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- fixed dose procedure
- Limit test:
- yes
- Specific details on test material used for the study:
- - Composition of test material, percentage of components: syringonitrile 99.8%
- Expiration date of the lot/batch: May 2010
-Physical description: pale yellow powder
-solubility: soluble in water
-stability: test substance was expected to be stable for the duration of the testing. - Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Received from Ace Animals, Inc, Boyertown, PA, on Nov. 25, 2008
- Age at study initiation: young adult (8-9 weeks)
-Initial weight: male -- 220-244 grams, female -- 178-199 grams
-Number of animals -- 5 male/5 female (non-pregnant)
- Housing: singly housed in suspended stainless steel cage
- Diet (e.g. ad libitum): pelleted Purina Rodent Chow
- Water (e.g. ad libitum): filtered tap water was supplied ad libitum by an automatic water dispensing system.
- Acclimation period: 9 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-22°C
- Humidity (%): 32-55%
- Photoperiod (hrs dark / hrs light):12 hr light/dark cycle - Type of coverage:
- semiocclusive
- Vehicle:
- water
- Details on dermal exposure:
- TEST SITE
- Area of exposure: 2x3 inch (approximately 10% of body surface)
- Type of wrap if used: 3 inch durapore tape wrapping the gauze and the entire trunk of each animal
REMOVAL OF TEST SUBSTANCE
- The gauze removed and the site cleaned gently of residual substance.
- Time after start of exposure: 24 hrs
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2000 mg/kg of body weight in a form of dry paste (65% w/w mixture in distilled water) - Duration of exposure:
- 24 hrs
- Doses:
- 2000 mg/kg of body weight
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Frequency of observations and weighing: observed for mortality, gross toxicity, and behaviour changes during the first several hours after application and at least once daily thereafter for 14 days. Weights recorded before the application, day 7 and on day 14.
- Necropsy of survivors performed: yes
- Other examinations performed: At necropsy, tissues and organs of thoracic and abdominal cavities were examined. - Preliminary study:
- All animals survived exposure to Mediator SNP. There were no overt signs of systemic toxicity throughout the 14-day observation period and at necropsy. There were no adverse effects on body weights and body weight gains. No gross abnormalities were noted at necropsy.
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Mortality:
- All animals survived exposure to the test substance.
- Clinical signs:
- other: No clinical observations other than the dermal irritations at 3 dose sites between days 1 and 3.
- Gross pathology:
- No gross abnormalities observed when necrospied at the end of the 14-day study.
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Under the conditions of this study, the single dose acute dermal LD50 of test substance is greater than 2000 mg/kg of body weight in male and female rats.
- Executive summary:
The objective of this study is to determine the potential for Mediator SNP (4-hydroxy-3,5-dimethoxybenzonitrile) to produce toxicity after topical application. The test material was moistened with distilled water and applied to the skin of 10 female rats at 2000 mg/kg body weight. The animals were observed for mortality, signs of systemic toxicity and behavioural changes daily for 14 consecutive days. Body weights were recorded and all animals were killed on day 14 post-dosing.
All animals survived exposure to Mediator SNP. There were no overt signs of systemic toxicity throughout the 14-day observation period and at necropsy. There were no adverse effects on body weights and body weight gains. No gross abnormalities were noted at necropsy. Under the conditions of this study, the acute dermal LD50 for Mediator SNP was greater than 2000 mg/kg body weight in female rats.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 2 000 mg/kg bw
Additional information
Acute Toxicity of 4 -hydroxy-3,5-dimethoxybenzonitrile (referred to as Mediator SNP)
Acute oral toxicity in rats – Fixed dose procedure
The objective of this study was to assess the acute toxicity of Mediator SNP when administered as a single oral dose followed by a 14-day period of observation. The information is used for both hazard assessment and ranking purposes. The study was initiated with an initial limit dose of 5000 mg/kg body weight in three female rats. The test substance was administered as a 35% w/w mixture in a 0.5% solution of carboxymethylcellulose (CMC) in distilled water.
No mortality was recorded in this study. There were no overt signs of systemic toxicity throughout the 14-day observation period and at necropsy. There were no adverse effects on body weights and body weight gains. No gross abnormalities were noted at necropsy.
Under the conditions of this study, the oral LD50 was > 5000 mg/kg body weight and Mediator SNP is classified as “practically non-toxic” by ingestion.
Acute dermal toxicity in rats – Limit Test
The objective of this study was to determine the potential for Mediator SNP to produce toxicity after topical application. The test material was moistened with distilled water and applied to the skin of 10 female rats at 2000 mg/kg body weight. The animals were observed for mortality, signs of systemic toxicity and behavioural changes daily for 14 consecutive days. Body weights were recorded and all animals were killed on day 14 post-dosing.
All animals survived exposure to Mediator SNP. There were no overt signs of systemic toxicity throughout the 14-day observation period and at necropsy. There were no adverse effects on body weights and body weight gains. No gross abnormalities were noted at necropsy.
Under the conditions of this study, the acute dermal LD50 for Mediator SNP was greater than 2000 mg/kg body weight in female rats. Mediator SNP can be classified as practically non toxic by dermal application.
Justification for classification or non-classification
Please see above.
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