Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 284-902-1 | CAS number: 84989-13-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
In vitro reverse gene mutation assay in bacteria: Negative; OECD 471; Dreher, 2017
In vitro cytogenicity/micronucleus in mammalian cells: Waiver
In vitro gene mutation in mammalian cells: Waiver
Link to relevant study records
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 March 2017 to 07 December 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Study was conducted in accordance with international guidelines and in accordance with GLP. All guideline validity criteria were met.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial forward mutation assay
- Specific details on test material used for the study:
- RADIOLABELLING INFORMATION (if applicable)
- Radiochemical purity: n/a
- Specific activity: n/a
- Locations of the label: n/a
- Expiration date of radiochemical substance: n/a
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Stable at room temperature
- Stability under test conditions: Not required
- Solubility and stability of the test substance in the solvent/vehicle: Not required
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: Not required
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: Test article stock solutions were prepared by the test article under subdued lighting in purified water with the aid of vortex mixing, ultrasonication and warming at 37 °C, to give the maximum required treatment concentration. The stock solutions were membrane filter-sterilised (Pall Acrodisc 32 mm/CR filter, 0.2 µm pore size) and subsequent dilutions were made using purified water. The test article solutions were protected from light and used within approximately 2 hours of initial formulation.
- Preliminary purification step (if any): n/a
- Final dilution of a dissolved solid, stock liquid or gel: 50, 16, 5, 1.6, 0.5, 0.16 and 0.05 mg/mL treatment solutions prepared.
- Final preparation of a solid: n/a
FORM AS APPLIED IN THE TEST (if different from that of starting material): Applied as an aqueous formulation.
OTHER SPECIFICS: n/a - Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Metabolic activation system:
- The mammalian liver post-mitochondrial fraction (S-9) used for metabolic activation was obtained from Molecular Toxicology Incorporated, USA where it was prepared from male Sprague Dawley rats induced with Aroclor 1254.
- Test concentrations with justification for top dose:
- Experiment 1 (S9 ±): 5, 16, 50, 160, 500, 1600, 5000 µg/plate
Experiment 2 (S9 -): 0.5, 1.6, 5, 16, 50, 160, 500 µg/plate
Experimetn 2 (S9 +): 15, 30, 60, 125, 250, 500, 1000 µg/plate
A maximum concentration of 5000 µg/plate was selected for Experiment 1, in order that initial treatments were performed up to this maximum recommended concentration according to current regulatory guidelines (OECD, 1997). For Experiment 2 the maximum concentration tested was selected on the basis of toxicity seen in the assay. - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: purified water
- Justification for choice of solvent/vehicle: Test item is completely soluble in water - Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- purified water
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- benzo(a)pyrene
- mitomycin C
- other: 2-aminoanthracene
- Details on test system and experimental conditions:
- Mutation Experiment
Benzenesulfonic acid, 4-C10-13-sec-alkyl derivs., ammonium salts was tested for mutation (and toxicity) in five strains of Salmonella typhimurium (TA98, TA100, TA1535, TA1537 and TA102), in two separate experiments, at the concentrations detailed previously, using triplicate plates without and with S-9 for test article, vehicle and positive controls. These platings were achieved by the following sequence of additions to molten agar at 45 ± 1 °C:
• 0.1 mL bacterial culture
• 0.1 mL of test article solution/vehicle control or 0.05 mL of positive control
• 0.5 mL 10% S-9 mix or buffer solution
This was followed by rapid mixing and pouring on to Vogel-Bonner E agar plates. When set, the plates were inverted and incubated at 37 ± 1 °C protected from light for 3 days. Following incubation, these plates were examined for evidence of toxicity to the background lawn, and where possible revertant colonies were counted (see Colony Enumeration Section 4.4).
As the results of Experiment 1 were negative, treatments in the presence of S-9 in Experiment 2 included a pre-incubation step in order to increase the sensitivity of the test system. Quantities of test article, vehicle control solution or positive control, bacteria and S-9 mix detailed above, were mixed together and incubated for 20 minutes at 37 ± 1 °C, with shaking, before the addition of 2 mL molten agar at 45 ± 1 °C. Plating of these treatments then proceeded as for the normal plate-incorporation procedure.
Toxicity Assessment
The background lawns of the plates were examined for thinning as sign of toxicity. Other evidence of toxicity may have included a marked reduction in revertants compared to the concurrent vehicle controls and/or a reduction in mutagenic response.
Colony Enumeration
Colonies were counted electronically using a Sorcerer Colony Counter (Perceptive Instruments) or manually where confounding factors such as bubbles or splits in the agar affected the accuracy of the automated counter. - Evaluation criteria:
- For valid data, the test article was considered to be mutagenic if:
1. A concentration related increase in revertant numbers was ≥1.5-fold (in strain TA102), ≥2-fold (in strains TA98 or TA100) or ≥3-fold (in strains TA1535 or TA1537) the concurrent vehicle control values
2. The positive trend/effects described above were reproducible.
The test article was considered positive in this assay if both of the above criteria were met.
The test article was considered negative in this assay if neither of the above criteria were met. - Statistics:
- Statistical analysis using Dunnett’s test was used to aid evaluation of a concentration response, up to limiting levels (for example toxicity, precipitation or 5000 µg/plate). However, adequate interpretation of biological relevance was of critical importance.
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Toxicity observed at 1600 µg/plate. Slightly thinned bacterial lawn observed at 500 µg/plate.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Toxicity observed at 500 µg/plate. Slight thinning of bacterial lawn at 160 µg/plate.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Toxicity observed at 1600 µg/plate. Slight thinning of bacterial lawn observed at 500 µg/plate.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Toxicity observed at 500 µg/plate. Slight thinning of bacterial lawn observed at 160 µg/plate.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Toxicity observed at 1600 µg/plate. Slight thinning of bacterial lawn observed at 500 µg/plate.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Toxicity observed at 500 µg/plate. Slight thinning of the bacterial lawn observed at 160 µg/plate.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Toxicity observed at 1600 µg/plate. Slight thinning of bacterial lawn observed at 500 µg/plate.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 102
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Toxicity observed at 500 µg/plate.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 102
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Toxicity observed at 5000 µg/plate. Very thin bacterial lawn present at 1600 µg/plate.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Effects of pH: not examined
- Effects of osmolality: not examined
- Evaporation from medium: not examined
- Water solubility: test item readily soluble at highest tested concentration
- Precipitation: not obsered
- Definition of acceptable cells for analysis: The inocula were taken from master plates or vials of frozen cultures, which had been checked for strain characteristics (histidine dependence, rfa character, uvrB character and resistance to ampicillin or ampicillin plus tetracycline).
- Other confounding effects: n/a
RANGE-FINDING/SCREENING STUDIES: no
CYTOKINESIS BLOCK (if used)
- Distribution of mono-, bi- and multi-nucleated cells: n/a
NUMBER OF CELLS WITH MICRONUCLEI
- Number of cells for each treated and control culture: n/a
- Indication whether binucleate or mononucleate where appropriate: n/a
HISTORICAL CONTROL DATA (with ranges, means and standard deviation and confidence interval (e.g. 95%)
- Positive historical control data: Yes
- Negative (solvent/vehicle) historical control data: Yes
ADDITIONAL INFORMATION ON CYTOTOXICITY:
- Measurement of cytotoxicity used: visual inspection of bacterial lawn
- Other observations when applicable: n/a - Conclusions:
- It was concluded that under the condition of this study, Benzenesulfonic acid, 4-C10-13-sec-alkyl derivs., ammonium salts did not induce mutation in five histidine-requiring strains (TA98, TA100, TA1535, TA1537 and TA102) of Salmonella typhimurium when tested under the conditions of this study. These conditions included treatments at concentrations up to 5000 µg/plate (the maximum recommended concentration according to current regulatory guidelines).
- Executive summary:
OECD 471 (2017) - In a reverse gene mutation assay in bacteria, strains of S. typhimurium (TA98, TA100, TA1535, TA1537 and TA102) were exposed to Benzenesulfonic acid, 4 -C10 -13 -sec-alkyl derivs., ammonium salts, formulated in purified water at concentration range of 0.5 – 5000 µg/plate in the presence and absence of mammalian metabolic activation S9-mix.
The test item was tested up to 5000 µg/plate (or its cytotoxic limit, where relevant), the maximum recommended concentration according to OECD 471. The positive controls induced the appropriate responses in the corresponding strains. There was no evidence (or a concentration related positive response) of induced mutant colonies over background in the test item treated plates.
This study is classified acceptable and satisfies the requirement for Test Guideline OECD 471 for in vitro mutagenicity (bacterial reverse gene mutation) data.
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- an in vitro cytogenicity study in mammalian cells or in vitro micronucleus study does not need to be conducted because adequate data from an in vivo cytogenicity test are available
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- other:
Referenceopen allclose all
Table 2: Mean number of revertants per plate (experiment 1 – pre-incubation not conducted)
Conc. (µg/plate) |
TA98 |
TA100 |
TA1535 |
TA1537 |
TA102 |
||||||||||
S9 - |
S9 + |
T (without / with S9 mix) |
S9 - |
S9 + |
T (without / with S9 mix) |
S9 - |
S9 + |
T (without / with S9 mix) |
S9 - |
S9 + |
T (without / with S9 mix) |
S9 - |
S9 + |
T (without / with S9 mix) |
|
Neg. control |
27.3 |
49.3 |
- / - |
124.3 |
89.3 |
- / - |
18.7 |
15.3 |
- / - |
12.7 |
8.0 |
- / - |
260.0 |
353.3 |
- / - |
5 |
21.7 |
33.0 |
- / - |
104.7 |
91.7 |
- / - |
12.7 |
16.0 |
- / - |
12.3 |
13.0 |
- / - |
279.0 |
344.0 |
- / - |
16 |
25.7 |
29.3 |
- / - |
95.7 |
107.3 |
- / - |
19.7 |
12.0 |
- / - |
7.5 |
14.0 |
- / - |
252.3 |
316.7 |
- / - |
50 |
29.3 |
39.0 |
- / - |
94.0 |
109.3 |
- / - |
16.3 |
12.3 |
- / - |
10.0 |
14.3 |
- / - |
254.3 |
362.7 |
- / - |
160 |
22.7 |
22.7 |
- / - |
62.3 |
95.3 |
S / - |
18.0 |
20.3 |
S / - |
8.7 |
9.3 |
S / - |
185.7 |
422.0 |
- / - |
500 |
9.0 |
25.0 |
S / S |
0 |
80.0 |
T / S |
0 |
15.0 |
T / S |
0 |
12.7 |
T / S |
0 |
342.0 |
T / - |
1600 |
0 |
0 |
T / T |
0 |
0 |
T / T |
0 |
0 |
T / T |
0 |
0 |
T / T |
0 |
116.0 |
T / V |
5000 |
0 |
0 |
T / T |
0 |
0 |
T / T |
0 |
0 |
T / T |
0 |
0 |
T / T |
0 |
0 |
T / T |
Pos. control |
545.3 |
102.0 |
- / - |
528.0 |
785.3 |
- / - |
631.7 |
224.0 |
- / - |
321.7 |
426.3 |
- / - |
904.3 |
3078.3 |
- / - |
T= cytotoxic, no sign of revertant colonies
- = no sign of cytotoxicity
S= slight reduction in bacterial lawn
V= very thin bacterial lawn
*= p<0.05
**= p<0.01
Table 3: Mean number of revertants per plate (experiment 2 – pre-incubation conducted)
Conc. (µg/plate) |
TA98 |
TA100 |
TA1535 |
TA1537 |
TA102 |
|||||
S9 - |
Toxicity |
S9 - |
Toxicity |
S9 - |
Toxicity |
S9 - |
Toxicity |
S9 - |
Toxicity |
|
Neg. control |
22.7 |
- |
100.0 |
- |
13.3 |
- |
10.0 |
- |
259.7 |
- |
0.5 |
25.7 |
- |
90.0 |
- |
14.7 |
- |
8.0 |
- |
325.3 |
- |
1.6 |
23.7 |
- |
78.0 |
- |
11.7 |
- |
14.7 |
- |
299.0 |
- |
5 |
25.7 |
- |
83.0 |
- |
11.3 |
- |
9.3 |
- |
305.7 |
- |
16 |
27.7 |
- |
98.7 |
- |
11.0 |
- |
6.0 |
- |
294.0 |
- |
50 |
30.3 |
- |
90.3 |
- |
21.0 |
- |
7.7 |
- |
260.7 |
- |
160 |
28.0 |
- |
75.3 |
- |
12.3 |
- |
6.3 |
- |
201.0 |
- |
500 |
22.3 |
- |
13.0 |
V |
10.7 |
- |
1.3 |
- |
126.7 |
S |
Pos. control |
591.0 |
- |
885.3 |
- |
642.0 |
- |
227.7 |
- |
722.0 |
- |
Conc. (µg/plate) |
TA98 |
TA100 |
TA1535 |
TA1537 |
TA102 |
|||||
S9 + |
Toxicity |
S9 + |
Toxicity |
S9 + |
Toxicity |
S9 + |
Toxicity |
S9 + |
Toxicity |
|
Neg. control |
33.3 |
- |
101.0 |
- |
18.0 |
- |
17.7 |
- |
298.3 |
- |
15 |
45.7* |
- |
109.7 |
- |
15.0 |
- |
16.0 |
- |
372.3* |
- |
30 |
33.7 |
- |
117.0 |
- |
13.0 |
- |
11.0 |
- |
390.3** |
- |
60 |
36.7 |
- |
88.3 |
- |
14.3 |
- |
10.3 |
- |
340.7 |
- |
125 |
31.0 |
- |
91.3 |
- |
13.7 |
- |
9.3 |
- |
360.0 |
- |
250 |
39.7 |
- |
82.3 |
- |
15.0 |
- |
11.0 |
- |
349.7 |
- |
500 |
29.7 |
- |
96.7 |
- |
17.7 |
- |
8.7 |
- |
244.7 |
- |
1000 |
19.0 |
- |
24.7 |
- |
14.3 |
- |
7.3 |
- |
218.7 |
- |
Pos. control |
303.0 |
- |
1296.3 |
- |
177.3 |
- |
427.0 |
- |
1552.0 |
- |
- = no sign of cytotoxicity
S= slight reduction in bacterial lawn
V= very thin background bacterial lawn
T= cytotoxic, no revertant colonies
*= p<0.05
**= p<0.01
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Genetic toxicity in vivo
Description of key information
In vivo mammalian bone marrow chromosome aberration test: Negative; Mammalian somatic cell cytogenetic assay (no guideline followed); Masubuchi, 1976 (read across from benzenesulfonic acid, 4-C10-13-sec-alkyl derivs. - data on LAS as part of category approach)
In vivo rodent dominant lethal assay: Negative; Rodent dominant lethal assay (no guideline followed); Masubchi, 1976 (read across from benzenesulfonic acid, 4-C10-13-sec-alkyl derivs. - data on LAS as part of category approach)
In vivo chromosome aberration assay: Negative; Chromosome aberration assay (no guideline followed); Anon., 1976 (read across from benzenesulfonic acid, 4-C10-13-sec-alkyl derivs. - data on LAS-Na salt as part of category approach)
In vivo mammalian bone marrow chromosome aberration test: Negative: Mammalian somatic cell cytogenetic assay (no guideline followed); Masubuchi, 1976 (read across from benzenesulfonic acid, 4-C10-13-sec-alkyl derivs. - data on LAS as part of category approach)
Link to relevant study records
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1976
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- Well-documented journal article.
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
Refer to Section 13.2 for read-across justification document. - Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- A group of 5 male mice was fed a diet containing 0.9% test substance for 9 months. At the end of this period, the animals were sacrificed, and the bone marrow cells examined for chromosome aberrations.
- GLP compliance:
- no
- Remarks:
- Study pre-dates GLP
- Type of assay:
- mammalian germ cell chromosome aberration test
- Species:
- mouse
- Strain:
- other: JCL-ICR
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: CLEA Japan, Inc.
- Age at study initiation: 4 weeks
- Housing: individually, except during breeding
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period:
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 25 +/- 1
- Humidity (%): 55 +/- 5
- Photoperiod (hrs dark / hrs light): 12/12 - Route of administration:
- oral: feed
- Details on exposure:
- DIET PREPARATION
- Mixing appropriate amounts with (Type of food): feed powder CE-2 - Duration of treatment / exposure:
- 9 months
- Frequency of treatment:
- Daily
- Dose / conc.:
- 1 170 mg/kg bw/day (nominal)
- Remarks:
- Basis: Nominal in diet
- No. of animals per sex per dose:
- 5
- Control animals:
- yes
- Tissues and cell types examined:
- femur bone marrow cells
- Details of tissue and slide preparation:
- Animals were sacrificed by administration of 1 ml/kg of 1% colchine solution. Femurs were then removed, and bone marrow cells washed into centrifuge tubes. The cells were then treated with 0.075 M KCl solution at 37 degree C for 15 min, and then fixed with an acetic acid 1: ethanol 3 solution. Samples were then flame dried and treated with Giemsa.
- Evaluation criteria:
- Presence and absence of chromosomal aberrations. 50 metaphases per individual.
- Statistics:
- Rohrborn's method.
- Sex:
- male
- Genotoxicity:
- negative
- Negative controls validity:
- valid
- Remarks on result:
- other: No increase in chromosome aberrations was noted.
- Additional information on results:
- No increase in chromosome aberrations was noted.
- Conclusions:
- Interpretation of results: negative
The test substance is not clastogenic. - Executive summary:
A group of 5 male mice was fed a diet containing 0.9% test substance for 9 months. At the end of this period, the animals were sacrificed, and the bone marrow cells examined for chromosome aberrations. No increase in chromosome aberrations was seen as compared to controls. The test substance is not clastogenic.
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
Refer to Section 13.2 for read-across justification document. - Reason / purpose for cross-reference:
- read-across source
- Sex:
- male
- Genotoxicity:
- negative
- Negative controls validity:
- valid
- Remarks on result:
- other: REPORTING FORMAT FOR THE ANALOGUE APPROACH Refer to Section 13.2 for read-across justification document.
- Conclusions:
- Interpretation of results: negative
The test substance is not clastogenic. - Executive summary:
In a one-to-one read-across approach, the substance benzenesulfonic acid, 4-C10-13-sec-alkyl derivs. (data on LAS as part of category approach) is considered appropriate for direct read-across (one-to-one) to benzenesulfonic acid, 4-C10-13-sec-alkyl derivs., ammonium salts (target substance) for the endpoint Genetic toxicity in vivo. A group of 5 male mice was fed a diet containing 0.9% test substance for 9 months. At the end of this period, the animals were sacrificed, and the bone marrow cells examined for chromosome aberrations. No increase in chromosome aberrations was seen as compared to controls.
In conclusion, The test substance in not clastogenic. A full justification for the read-across approach is presented in IUCLID Section 13.2.
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1976
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- Well-documented journal article.
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
Refer to Section 13.2 for read-across justification document. - Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Groups of 5 male rats were fed a diet containing 0.9% test substance for 9 months. At the end of this period, the animals were sacrificed, and the bone marrow cells examined for chromosome aberrations.
- GLP compliance:
- no
- Remarks:
- Study pre-dates GLP
- Type of assay:
- mammalian bone marrow chromosome aberration test
- Species:
- rat
- Strain:
- other: Wistar and SD
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: CLEA Japan, Inc.
- Age at study initiation: 4 weeks
- Housing: individually
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period:
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 25 +/- 1
- Humidity (%): 55 +/- 5
- Photoperiod (hrs dark / hrs light): 12/12 - Route of administration:
- oral: feed
- Details on exposure:
- DIET PREPARATION
- Mixing appropriate amounts with (Type of food): feed powder CE-2 - Duration of treatment / exposure:
- 9 months
- Frequency of treatment:
- Daily
- Dose / conc.:
- 450 mg/kg bw/day (nominal)
- Remarks:
- Nominal in diet
- No. of animals per sex per dose:
- 5
- Control animals:
- yes
- Tissues and cell types examined:
- femur bone marrow cells
- Details of tissue and slide preparation:
- Animals were sacrificed by administration of 1 ml/kg of 1% colchine solution. Femurs were then removed, and bone marrow cells washed into centrifuge tubes. The cells were then treated with 0.075 M KCl solution at 37 degree C for 15 min, and then fixed with an acetic acid 1: ethanol 3 solution. Samples were then flame dried and treated with Giemsa.
- Evaluation criteria:
- Presence and absence of chromosomal aberrations. 50 metaphases per individual.
- Statistics:
- Rohrborn's method.
- Sex:
- male
- Genotoxicity:
- negative
- Negative controls validity:
- valid
- Remarks on result:
- other: No increase in chromosome aberrations was noted.
- Conclusions:
- Interpretation of results (migrated information): negative
The test substance is not clastogenic. - Executive summary:
Groups of 5 male rats were fed a diet containing 0.9% test substance for 9 months. At the end of this period, the animals were sacrificed, and the bone marrow cells examined for chromosome aberrations. No increase in chromosome aberrations was seen as compared to controls. The test substance is not clastogenic.
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
Refer to Section 13.2 for read-across justification document. - Reason / purpose for cross-reference:
- read-across source
- Sex:
- male
- Genotoxicity:
- negative
- Negative controls validity:
- valid
- Remarks on result:
- other: No increase in chromosome aberrations was noted.
- Conclusions:
- Interpretation of results (migrated information): negative
The test substance is not clastogenic. - Executive summary:
In a one-to-one read-across approach, the substance benzenesulfonic acid, 4-C10-13-sec-alkyl derivs. (data on LAS as part of category approach) is considered appropriate for direct read-across (one-to-one) to benzenesulfonic acid, 4-C10-13-sec-alkyl derivs., ammonium salts (target substance) for the endpoint Genetic toxocity in vivo. In conclusion, no increase in chromosome aberrations was seen as compared to controls following exposure of 5 male rats fed with a diet containing 0.9% test substance for 9 months. The test substance is not clastogenic. A full justification for the read-across approach is presented in IUCLID Section 13.2.
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1976
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- Well-documented publication.
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
Refer to Section 13.2 for read-across justification document. - Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Groups of male mice were given doses of 200, 400, or 800 mg/kg of Benzenesulfonic acid, C10-14-alkyl derivs., sodium salts. At 6, 24, and 48 hrs, 3 of the mice from each dosage group were sacrificed. The bone marrow cells from the femurs were collected and examined for chromosome aberrations. In addition, one group of mice was exposed daily for 5 consecutive days. Additional groups of mice were exposed to commerical detergents containing 19% or 17.1% of the test substance. Mitomycin C was used as a positive control.
- GLP compliance:
- no
- Remarks:
- Study pre-dates GLP
- Type of assay:
- mammalian bone marrow chromosome aberration test
- Species:
- mouse
- Strain:
- other: ICR/JCL
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: CLEA Japan Inc.
- Age at study initiation: 9-11 weeks
- Housing: individually in plastic cages
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): tap water ad libitum
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 23 +/- 2
- Humidity (%): 55 +/- 5
- Photoperiod (hrs dark / hrs light): 12/12 hrs - Route of administration:
- oral: gavage
- Vehicle:
- Vehicle(s)/solvent(s) used: distilled water
- Details on exposure:
- PREPARATION OF DOSING SOLUTIONS: Samples were diluted to make a 5 ml/kg dose volume
- Duration of treatment / exposure:
- Single treatment, except for one group which was given 5 consecutive daily exposures to the test substance
- Frequency of treatment:
- Once, daily
- Remarks:
- 200, 400, 800 mg/kg
Basis:
nominal conc.
test substance - Remarks:
- 800, 1600, 3200 mg/kg
Basis:
nominal conc.
commercial detergent containing 19% test substance - Remarks:
- 1000, 2000, 4000 mg/kg
Basis:
nominal conc.
commercial detergent containing 17.1% test substance - No. of animals per sex per dose:
- 9
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- mitomycin C1
- Route of administration: intraperitoneally
- Doses / concentrations: 5 mg/kg - Tissues and cell types examined:
- Bone marrow cells from femurs
- Details of tissue and slide preparation:
- CRITERIA FOR DOSE SELECTION:
TREATMENT AND SAMPLING TIMES ( in addition to information in specific fields): 3 animals were sacrificed at 6, 24, and 48 hrs after treatment. One group was exposed daily for 5 days prior to sacrifice.
DETAILS OF SLIDE PREPARATION: Cells were placed in Hank's solution, then centrifuged at 1000 rpm for 5 min. Supernatant was discarded, and 5 ml of 0.075 M KCl was added. The mixture then stood for 5 min., then was stirred and centrifuged again. This was repeated several times, before finally placing one or two drops on the slides, and staining with 2% Giemsa solution. - Evaluation criteria:
- number of cells with chromatid and chromosome gaps, number of cells with aberrations
50 metaphases per animal (150 total) were imaged at each time point. - Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- not examined
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: No significant differences in the incidence of chromosomal aberrations were observed in any test substance treatment group relative to the controls.
- Additional information on results:
- No significant differences in the incidence of chromosomal aberrations were observed in any test substance treatment group relative to the controls.
- Conclusions:
- Interpretation of results: negative
The test substance is not clastogenic. - Executive summary:
Groups of male mice were given doses of 200, 400, or 800 mg/kg of Benzenesulfonic acid, C10-14-alkyl derivs., sodium salts. At 6, 24, and 48 hrs, 3 of the mice from each dosage group were sacrificed. The bone marrow cells from the femurs were collected and examined for chromosome aberrations. In addition, one group of mice was exposed daily for 5 consecutive days. Additional groups of mice were exposed to commerical detergents containing 19% or 17.1% of the test substance. Mitomycin C was used as a positive control. None of the treatment groups showed any significant increase in chromosome aberrations as compared to negative controls. The test substance in not clastogenic.
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
Refer to Section 13.2 for read-across justification document. - Reason / purpose for cross-reference:
- read-across source
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- not examined
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: No significant differences in the incidence of chromosomal aberrations were observed in any test substance treatment group relative to the controls.
- Conclusions:
- Interpretation of results: negative
The test substance is not clastogenic. - Executive summary:
In a one-to-one read-across approach, the substance benzenesulfonic acid, 4-C10-13-sec-alkyl derivs. (data on LAS-Na salt as part of category approach) is considered appropriate for direct read-across (one-to-one) to benzenesulfonic acid, 4-C10-13-sec-alkyl derivs., ammonium salts (target substance) for the endpoint Genetic toxicity in vivo. Groups of male mice were given doses of 200, 400, or 800 mg/kg of Benzenesulfonic acid, C10-14-alkyl derivs., sodium salts. At 6, 24, and 48 hrs, 3 of the mice from each dosage group were sacrificed. The bone marrow cells from the femurs were collected and examined for chromosome aberrations. In addition, one group of mice was exposed daily for 5 consecutive days. Additional groups of mice were exposed to commerical detergents containing 19% or 17.1% of the test substance. Mitomycin C was used as a positive control. None of the treatment groups showed any significant increase in chromosome aberrations as compared to negative controls.
In conclusion, The test substance in not clastogenic. A full justification for the read-across approach is presented in IUCLID Section 13.2.
- Endpoint:
- in vivo mammalian germ cell study: cytogenicity / chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1976
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- Well-documented journal article.
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
Refer to Section 13.2 for read-across justification document. - Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- A group of 7 male mice was fed a diet containing 0.6% test substance for 9 months. At the end of this period, the animals were each mated with two untreated females. On day 13 of pregnancy, the females were sacrificed, and the ovaries and uteri were examined.
- GLP compliance:
- no
- Type of assay:
- rodent dominant lethal assay
- Species:
- mouse
- Strain:
- other: JCL-ICR
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: CLEA Japan, Inc.
- Age at study initiation: 4 weeks
- Housing: individually, except during breeding
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 25 +/- 1
- Humidity (%): 55 +/- 5
- Photoperiod (hrs dark / hrs light): 12/12 - Route of administration:
- oral: feed
- Details on exposure:
- DIET PREPARATION
- Mixing appropriate amounts with (Type of food): feed powder CE-2 - Duration of treatment / exposure:
- 9 months
- Frequency of treatment:
- Daily
- Dose / conc.:
- 300 mg/kg bw/day (nominal)
- Remarks:
- Nominal in diet
- No. of animals per sex per dose:
- 7
- Control animals:
- yes
- Statistics:
- Rohrborn's method.
- Sex:
- male
- Genotoxicity:
- negative
- Negative controls validity:
- valid
- Remarks on result:
- other: There were no significant differences in fertility, the mortality of ova and embryos, the number of surviving fetuses, or the index of dominant lethal induction between the experimental groups and the control group.
- Conclusions:
- Interpretation of results: negative
The test substance did not cause genetic disorders in mice. - Executive summary:
A group of 7 male mice was fed a diet containing 0.6% test substance for 9 months. At the end of this period, the animals were each mated with two untreated females. On day 13 of pregnancy, the females were sacrificed, and the ovaries and uteri were examined. No increase in dominant lethal induction was seen as compared to controls. The test substance does not cause genetic disorders.
- Endpoint:
- in vivo mammalian germ cell study: cytogenicity / chromosome aberration
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
Refer to Section 13.2 for read-across justification document. - Reason / purpose for cross-reference:
- read-across source
- Sex:
- male
- Genotoxicity:
- negative
- Negative controls validity:
- valid
- Remarks on result:
- other: There were no significant differences in fertility, the mortality of ova and embryos, the number of surviving fetuses, or the index of dominant lethal induction between the experimental groups and the control group.
- Conclusions:
- Interpretation of results: negative
The test substance did not cause genetic disorders in mice. - Executive summary:
In a one-to-one read-across approach, the substance benzenesulfonic acid, 4-C10-13-sec-alkyl derivs. (data on LAS as part of category approach) is considered appropriate for direct read-across (one-to-one) to benzenesulfonic acid, 4-C10-13-sec-alkyl derivs., ammonium salts (target substance) for the endpoint Genetic toxicity in vivo. In conclusion, no increase in dominant lethal induction was seen as compared to controls following 9 months dietary exposure to males at 300 mg/kg bw/d, followed by mating with untreated females. On day 13 of pregnancy, the females were sacrificed, and the ovaries and uteri were examined. The test substance does not cause genetic disorders. A full justification for the read-across approach is presented in IUCLID Section 13.2.
Referenceopen allclose all
Chromosome Aberrations
1170 mg/kg bw/d in diet | Control | |
Number of cells with chromatid breaks | 1 | 2 |
Number of cells with isochromatid breaks | 1 | 0 |
Number of cells with chromatid gaps | 4 | 5 |
Number of cells with isochromatic gaps | 0 | 0 |
Number of cells with other aberrations | 0 | 0 |
Chromosome Aberrations
450 mg/kg diet - Wistar Rats | 450 mg/kg diet - SD Rats | Control | Control | |
Number of cells with chromatid breaks | 0 | 0 | 1 | 0 |
Number of cells with isochromatid breaks | 0 | 0 | 0 | 0 |
Number of cells with chromatid gaps | 3 | 4 | 3 | 4 |
Number of cells with isochromatid gaps | 0 | 0 | 0 | 0 |
Number of cells with other aberrations | 0 | 0 | 0 | 0 |
Chromosome Aberrations
Total number of cells having aberrations and occurrence (%) | ||||
6 hrs | 24 hrs | 48 hrs | 5 days | |
200 mg/kg | 0 (0) | 0 (0) | 0 (0) | 1 (0.7) |
400 mg/kg | 1 (0.7) | 0 (0) | 0 (0) | 0 (0) |
800 mg/kg | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
800 mg/kg of 17.1 % detergent | 0 (0) | 0 (0) | 0 (0) | - |
1600 mg/kg of 17.1 % detergent | 0 (0) | 1 (0.7) | 0 (0) | - |
3200 mg/kg of 17.1 % detergent | 2 (1.3) | 2 (1.3) | 0 (0) | - |
1000 mg/kg of 19 % detergent | - | 0 (0) | - | - |
2000 mg/kg of 19 % detergent | - | 0 (0) | - | - |
4000 mg/kg of 19 % detergent | - | 0 (0) | - | - |
mitomycon C | 16 (10.7) | 53 (353) | 13 (8.7) | 112 (74.7) |
Distilled water | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
Untreated | 0 (0) | 0 (0) | 1 (0.7) | 0 (0) |
Dominant Lethal Assay Results
0.6 % in diet (300 mg/kg bw/day) | Control | |
Number of mating females | 14 | 18 |
Number pregnant | 11 | 12 |
Number with dead embryos | 6 | 10 |
Dead embryos per pregnant female | 54.6 % | 83.3 % |
Number of corpora lutea | 156 | 161 |
Corpora lutea per pregnant female | 14.2 | 13.4 |
Number of implants | 148 | 156 |
Implants per pregnant female | 13.5 | 13.0 |
Implants per corpora lutea | 94.9 | 96.9 |
Number of live fetuses | 142 | 143 |
Live fetuses per pregnant female | 12.9 | 11.9 |
Live fetuses per corpora lutea | 91.0 | 88.8 |
Live fetuses per total implants | 96.0 | 91.7 |
Number of early dead fetuses | 4 | 12 |
Number of late dead fetuses | 2 | 1 |
% of dominant lethals | -4.67 | - |
% of dominant lethals | -8.33 | - |
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Mode of Action Analysis / Human Relevance Framework
Not applicable, no adverse effects observed.
Additional information
Genetic Toxicity - in vitro
OECD 471 (2017) - In a reverse gene mutation assay in bacteria, strains of S. typhimurium (TA98, TA100, TA1535, TA1537 and TA102) were exposed to Benzenesulfonic acid, 4 -C10 -13 -sec-alkyl derivs., ammonium salts, formulated in purified water at concentration range of 0.5 – 5000 µg/plate in the presence and absence of mammalian metabolic activation S9-mix. The test item was tested up to 5000 µg/plate (or its cytotoxic limit, where relevant), the maximum recommended concentration according to OECD 471. The positive controls induced the appropriate responses in the corresponding strains. There was no evidence (or a concentration related positive response) of induced mutant colonies over background in the test item treated plates.
Genetic Toxicity - in vivo
Masubuchi (1976) CAT - In a one-to-one read-across approach, the substance benzenesulfonic acid, 4-C10-13-sec-alkyl derivs. (data on LAS as part of category approach) is considered appropriate for direct read-across (one-to-one) to benzenesulfonic acid, 4-C10-13-sec-alkyl derivs., ammonium salts (target substance) for the endpoint Genetic toxocity in vivo. In conclusion, no increase in chromosome aberrations was seen as compared to controls following exposure of 5 male rats fed with a diet containing 0.9% test substance for 9 months. The test substance is not clastogenic.
Masubuchi (1976) RDLA - In a one-to-one read-across approach, the substance benzenesulfonic acid, 4-C10-13-sec-alkyl derivs. (data on LAS as part of category approach) is considered appropriate for direct read-across (one-to-one) to benzenesulfonic acid, 4-C10-13-sec-alkyl derivs., ammonium salts (target substance) for the endpoint Genetic toxicity in vivo. In conclusion, no increase in dominant lethal induction was seen as compared to controls following 9 months dietary exposure to males at 300 mg/kg bw/d, followed by mating with untreated females. On day 13 of pregnancy, the females were sacrificed, and the ovaries and uteri were examined. The test substance does not cause genetic disorders.
Anon. (1976) CAT - In a one-to-one read-across approach, the substance benzenesulfonic acid, 4-C10-13-sec-alkyl derivs. (data on LAS-Na salt as part of category approach) is considered appropriate for direct read-across (one-to-one) to benzenesulfonic acid, 4-C10-13-sec-alkyl derivs., ammonium salts (target substance) for the endpoint Genetic toxicity in vivo. Groups of male mice were given doses of 200, 400, or 800 mg/kg of Benzenesulfonic acid, C10-14-alkyl derivs., sodium salts. At 6, 24, and 48 hrs, 3 of the mice from each dosage group were sacrificed. The bone marrow cells from the femurs were collected and examined for chromosome aberrations. In addition, one group of mice was exposed daily for 5 consecutive days. Additional groups of mice were exposed to commerical detergents containing 19% or 17.1% of the test substance. Mitomycin C was used as a positive control. None of the treatment groups showed any significant increase in chromosome aberrations as compared to negative controls.
In conclusion, The test substance in not clastogenic.
Masubuchi (1976) CAT - In a one-to-one read-across approach, the substance benzenesulfonic acid, 4-C10-13-sec-alkyl derivs. (data on LAS as part of category approach) is considered appropriate for direct read-across (one-to-one) to benzenesulfonic acid, 4-C10-13-sec-alkyl derivs., ammonium salts (target substance) for the endpoint Genetic toxicity in vivo. A group of 5 male mice was fed a diet containing 0.9% test substance for 9 months. At the end of this period, the animals were sacrificed, and the bone marrow cells examined for chromosome aberrations. No increase in chromosome aberrations was seen as compared to controls.
In conclusion, The test substance in not clastogenic.
Justification for classification or non-classification
The substance does not meet the criteria for classification in accordance with Regulation (EC) No 1272/2008.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.