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EC number: 266-803-5 | CAS number: 67634-00-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- (Modified Sturm Test)”, adopted on 17 July 1992.
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic, adapted
- Details on inoculum:
- The inoculum was secondary effluent, collected from a treatment plant receiving predominantly domestic sewage. This effluent was used as test system as it is recommended in the guideline. A fresh sample of secondary effluent was collected from the treatment plant and was kept aerobic during transport.
This effluent was allowed to settle for one hour, decanted and the decanted effluent was used in the test.
Source of the Inoculum
Sewage Treatment Plant
Eurofins Advinus Limited
Bengaluru – 560 058
India
Determination of Bacterial Population in the Inoculum
The bacterial population in the inoculum was determined as colony forming units (CFU/mL) by diluting the inoculum to an appropriate dilution and then plating on nutrient agar plates.
Preconditioning of the Inoculum
The decanted effluent was preconditioned by aerating for 5 days at 22 to 24C. - Duration of test (contact time):
- ca. 28 d
- Initial conc.:
- ca. 240 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- Preparation of Test Flasks
Flask No. Contents
1 & 2 Test suspension – test item and inoculum
3 & 4 Inoculum blank – only inoculum
5 Procedure control – reference item and inoculum
6 Toxicity control – test item, reference item and inoculum
To each 5 L flask, 2400 mL of mineral medium was added and mixed with 300 mL of the pre-conditioned inoculum. A separate 3000 mL of mineral medium was also prepared in a flask to use it for further dilutions.
A sample of the mineral medium was checked for the inorganic carbon content.
These flasks were aerated with CO2 free air at 43 to 50 mL/minute, overnight to purge the system of carbon dioxide. - Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 75.18 - <= 81.05
- St. dev.:
- 4.15
- Sampling time:
- 13 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 52.33 - <= 58.46
- Sampling time:
- 10 d
- Details on results:
- 8.3 Production of Carbon-dioxide during the Treatment Period
The carbon dioxide production during the treatment period is given in Table 1. pH of the test solutions at the end of the test is given in Table 2.
The mean carbon dioxide produced from the inoculum blank (Flask No. 3 and 4) on Days 3, 6, 8, 10, 13, 16, 20, 24, 27 and 29 after the treatment was 20.63, 16.61, 11.88, 26.68, 14.80, 11.77, 10.89, 10.01 and 7.81 mg, respectively. The total carbon dioxide produced from the inoculum blank for throughout the test period was 43.69 mg/L.
The cumulative carbon dioxide produced from the test item was 15.56, 40.64, 71.77, 99.76, 138.31, 178.79, 214.65, 248.97 and 281.31 mg in Flask 1 on Days 3, 6, 8, 10, 13, 16, 20, 24, 27 and 29 after the treatment, respectively. Similarly, in Flask 2 it was 12.81, 31.62, 61.43, 89.31, 128.30, 168.34, 204.09, 238.19 and 271.41 mg on Days 3, 6, 8, 10, 13, 16, 20, 24, 27 and 29 after the treatment, respectively.
The cumulative carbon dioxide produced from the reference item (Flask No. 5) was 29.97, 65.61, 103.78, 100.31, 125.55, 141.83, 159.76, 183.41 and 199.69 mg, on Days 3, 6, 8, 10, 13, 16, 20, 24, 27 and 29 after the treatment, respectively.
The cumulative carbon dioxide produced from the toxicity control (Flask No. 6) was 21.17, 50.98, 82.55, 71.16, 93.87, 114.66, 128.08, 144.03 and 157.78 mg, on Days 3, 6, 8, 10, 13, 16, 20, 24, 27 and 29 after the treatment, respectively.
The pH of the test solutions at the end of the test was 7.81, 7.82, 7.81, 7.82, 7.81 and 7.82 in Flask 1, 2, 3, 4, 5 and 6 respectively.
8.4 Percent Degradation
The percent degradation of the test item, reference item and toxicity control is presented in Table 3 and Figure 1.
The percent degradation of test item was 164.84% and 159.04% in the Flask No. 1 and 2, respectively at the end of the test. The mean per cent degradation of test item was 161.94% at the end of test while, the percent degradation of reference item was 119.32% and the toxicity control was 93.66% at the end of the test. It was observed that the mean degradation of test item reached 78.12% on Day 13 after reaching 21.17% on Day 6. The degradation of test item, Allyl Amyl Glycolate reached more than 60% pass level in 10-day window period after reaching 10% of ThCO2 production and within the 28-d period of the test.
Based on the results, the test item was concluded as readily biodegradable as 78.12% mean degradation was achieved in between Days 6 and 13, crossing 60% pass level in 10-day window period after reaching 10% of ThCO2 production and within the 28-d period of the test. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Based on the results, the test item was concluded as readily biodegradable as 78.12% mean degradation was achieved between Days 6 and 13, crossing 60% pass level in 10-day window period after reaching 10% of ThCO2production and within the 28-d period of the test.
- Executive summary:
The ready biodegradability of Allyl Amyl Glycolate was tested using the CO2 Evolution Test. The test item was added to two test vessels at the concentration of 24 mg/L (equivalent to 15.5 mg of Total Organic Carbon/L). Two control treatments containing only the inoculum, one reference item treatment and one toxicity control treatment containing the test item and the reference item were also tested. All the treatments were added with equal volume of inoculum which was collected from the secondary effluent treatment plant receiving predominantly domestic sewage.
Treatment mixtures were aerated for 29 days with carbon dioxide (CO2) free air. The CO2 released was trapped in a series of bottles containing barium hydroxide, which were connected to the outlet of each test vessel. The residual barium hydroxide was measured on Days 3, 6, 8, 10, 13, 16, 20, 24, 27 and 29 after the initiation of the test.
The mean per cent degradation of test item was 161.94% at the end of test while, the percent degradation of reference item was 119.32% and the toxicity control was 93.66% at the end of the test. It was observed that the mean degradation of test item reached 78.12% on Day 13 after reaching 21.17% on Day 6. The degradation of test item, Allyl Amyl Glycolate reached more than 60% pass level in 10-day window period after reaching 10% of ThCO2 production and within the 28-d period of the test.
The test fulfilled all the validity criteria.
Based on the results, the test item was concluded as readily biodegradable as 78.12% mean degradation was achieved between Days 6 and 13, crossing 60% pass level in 10-day window period after reaching 10% of ThCO2 production and within the 28-d period of the test.
Reference
Description of key information
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
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