Benzenamine, 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]-, N-Me derivatives, molybdatephosphates

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

06 March 2000 - 10 march 2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

limited to screening with exposure of 5 fish per concentration to four levels of loading, and di not include analytical support

GLP compliance:

not specified

Specific details on test material used for the study:

Storage: room temp in the dark
Purity: 100%
Description: violet powder

Analytical monitoring:

no

Details on sampling:

- Concentrations: 0.1, 1, 10 , 100 mg/L

Vehicle:

not specified

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: prepared separately and expressed as whole water fractions
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): undissolved particles at 10 and 100 mg/L

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: Bio international, St. anthonis, the netherlands
- Maintenance of the brood fish: ISO-medium

ACCLIMATION
- Acclimation period: 12 days after delivery
- Acclimation conditions (same as test or not): same

Test type:

static

Water media type:

other: ISO medium

Limit test:

yes

Total exposure duration:

96 h

Remarks on exposure duration:

not specified

Post exposure observation period:

not specified

Hardness:

not specified

Test temperature:

22±1 °C

pH:

7.4-8.4

Dissolved oxygen:

6mg O2/L

Salinity:

not specified

Nominal and measured concentrations:

.01, 1 , 10 , 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 4 litre glass vessels
- Aeration: 24h to end of experiment
- Type of flow-through (e.g. peristaltic or proportional diluter): static
- No. of organisms per vessel: 5
- Biomass loading rate: 0.63g fish/litre i.e 5 fish per 2 litres of test medium
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: millipore corp, bedford, usa
- Metals: Magnesium 12mg/L, Sodium 15mg/L, potassium 3mg/L
- Pesticides: 49mg/L
- Chlorine: 145mg/L
OTHER TEST CONDITIONS
- Photoperiod: 16 hours daily

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Remarks:

0347N

Effect conc.:

>= 10 - <= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Remarks:

0345N

Effect conc.:

ca. 5.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Remarks:

0348N

Effect conc.:

ca. 16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: precipitation observed in all test solutions from 1 to 100mg/L from start of test

Results with reference substance (positive control):

not applicable

Reported statistics and error estimates:

none

Validity criteria fulfilled:

not applicable

Conclusions:

No effects of zebrafish were observed when fish were exposed to 0345N, 0347N, and 0348N loadings of 0.1 or 1.0mg/L

Description of key information

No effects on zebrafish were observed when fish were exposed to 0345N, 0347N, and 0348N loadings of 0.1 or 1.0mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

5.9 mg/L