Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 253-518-6 | CAS number: 37475-84-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Remarks:
- the tested substance is AMP one of the components of the compound
- Adequacy of study:
- key study
- Study period:
- Not applicable
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: meets generally accepted scientific standards, well-documented, and acceptable for assessment
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- other: Methods of Acute Toxicity Tests with Fish, Macroinvertebrates, and Amphibians" (USEPA, 1975
- Deviations:
- no
- Remarks:
- Not specified in report
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Deionized water was used in the preparation of all stock solutions.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnids (Daphnia magna) first instar < 24 hours old. The daphnia used in the test were cultured at the testing facilities, and the adult daphnia were fed a suspension of trout chow and alfalfa daily until 24 hours prior to testing.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 225 ppm as CaCO3
- Test temperature:
- 19-20C
- pH:
- 8.1 in the control, 8.3 (100mg/L AMP), 8.9 (320 mg/L AMP), and 9.4 (1000 mg/L AMP)
- Dissolved oxygen:
- Dissolved oxygen levels ranging from 8.3 mg/L in the control at study start, and levels of 8.5 (control), 8.5 (100mg/L AMP), 8.6 (320 mg/L AMP), and 8.6 mg/L O2 (1000 mg/L AMP) at 48 hours.
- Nominal and measured concentrations:
- Nominal- 0, 100, 180, 320, 560, and 1000 mg/L AMP-95
- Details on test conditions:
- Daphnias (Daphnia magna) first instar < 24 hours old. Test conditions were a temperature range of 19-20C, and a photoperiod of 16 hours light/ 8 hour dark photcycle. Test solution (200mL) was placed in 250mL beakers, and ten daphnias were added per test solution.
- Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 193 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL: 100-320 mg/L
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 240 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL: 212-272 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- There was no additional data provided on the results of the range-finding study referenced in the methods section.
- Reported statistics and error estimates:
- A computerized program calculated the LC50 for Daphnia based on the Probit dose-response model.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 48-hour Daphnia magna LC50 was calculated to be 193 mg/L.
- Executive summary:
AMP-95 (P-2031, 2-amino-2-methyl-propanol), lot number 216 (AMP-94.31%, Water 5.69%, NVM 0.0004%, Neutral Eq. 94.68), was tested for the acute t toxicity effects on daphnias. The 24 and 48 hr LC50 and "no effect" levels were determined by ABC Laboratories, Inc., Columbia, Missouri. Test Conditions-daphnias (Daphnia magna) first instar ≤24 hr old. Test temperature 19-20C. Photoperiodicity 16 hr day light. 200 ml test solution in 250 ml beakers. Ten daphnias per test solution. The AMP-95 concentrations were tested in duplicate at 0, 100, 180, 320, 560 and 1000 mg/liter (ppm). Results- There was no significant changes in the water quality parameters except an increase in pH at the ≥320 mg/l concentration. The estimated LC50 at 24 hr was 240 (212-272) mg/liter and at 48 hr was 193 (100-320) mg/liter (95 percent confidence limits are in parenthesis). The 48 hr “no effect” level was 100 mg/liter.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Remarks:
- the tested substance is DDBSA one of the components of the compound
- Adequacy of study:
- key study
- Study period:
- May 8, 2000
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species: Daphnia magna, <24 h old.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20±1 degree C
- pH:
- pH 7.8-8.0
- Dissolved oxygen:
- dissolved oxygen 7.7-8.0;
- Nominal and measured concentrations:
- 0.4, 0.7, 1.2, 2.0, 3.5, 6.0, and 10 mg/L
- Details on test conditions:
- Seven concentrations and a control were tested (0.4, 0.7, 1.2, 2.0, 3.5, 6.0, and 10 mg/L). Physicochemical parameters were maintained within acceptable ranges (temperature 20±1 degree C; dissolved oxygen 7.7-8.0; pH 7.8-8.0). At 48 hours, 0/20 daphnids were immobile in the 0.4 through 3.5 mg/L concentrations, 14/20 in 6.0 mg/L, and 20/20 in 10 mg/L. Graphical interpolation was used to calculate the EC50 value.
- Reference substance (positive control):
- not required
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: (95% 3.5-6.0 mg/L)
- Details on results:
- A 48 h EC50 of 5.2 mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 48 h EC50 of 5.2 mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.
- Executive summary:
A 48 h EC50 of 5.2 mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.
Referenceopen allclose all
Not applicable
Description of key information
For DDBSA a 48 h EC50 of 5.2 mg/L has been determined for the effects on Immobility of the Daphnia magna.
AMP-95 (P-2031, 2-amino-2-methyl-propanol), lot number 216 (AMP-94.31%, Water 5.69%, NVM 0.0004%, Neutral Eq. 94.68), was tested for the acute t toxicity effects on daphnia. The 24 and 48 hr LC50 and "no effect" levels were determined by ABC Laboratories, Inc., Columbia, Missouri. Test Conditions-daphnia (Daphnia magna) first instar ≤24 hr old. Test temperature 19-20C. Photoperiodicity 16 hr day light. 200 ml test solution in 250 ml beakers. Ten daphnia per test solution. The AMP-95 concentrations were tested in duplicate at 0, 100, 180, 320, 560 and 1000 mg/L (ppm). Results- There was no significant changes in the water quality parameters except an increase in pH at the ≥320 mg/L concentration. The estimated LC50 at 24 hr was 240 (212-272) mg/L and at 48 hr was 193 (100-320) mg/L(95 percent confidence limits are in parenthesis). The 48 hr “no effect” level was 100 mgL.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 5.2 mg/L
Additional information
Data on both dissociation products DDBSA and AMP are available. In a worst case approach it is assumed that the toxicity to daphnia is determined by DDBSA, which leads to a LC50 of 5.2 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.