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Diss Factsheets
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EC number: 213-720-7 | CAS number: 1004-38-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- non-GLP screening study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Principles of method if other than guideline:
- This study is a screening test and does not include all aspects required by the standard test guidelines.
Main modifications:
- Reduced number of test concentrations (3) with a spacing factor of 10
- No concentration control analysis
- No statistical evaluation - GLP compliance:
- no
- Remarks:
- This study does not have a GLP status. However, the experimental conduct of this study was carried out in general in accordance with the OECD GLP Principles, the GLP Principles of the German Chemical Act and, as far as applicable, by laboratory SOPs.
Test material
- Reference substance name:
- Pyrimidine-2,4,6-triyltriamine
- EC Number:
- 213-720-7
- EC Name:
- Pyrimidine-2,4,6-triyltriamine
- Cas Number:
- 1004-38-2
- Molecular formula:
- C4H7N5
- IUPAC Name:
- 2,4,6-Triaminopyrimidine
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Test substance preparation:
Stock solution: 100.2 mg of the test substance were dissolved with M4 media to 1L. The stock solution appeared clear and colorless (correspond to test
concentration of 100 mg/L).
Test solution 1: 10 mL of the stock solution were diluted with M4 media to 1 L (correspond to test concentration of 10 mg/L).
Test solution 2: 10 mL of the test solution 1 were diluted with M4 media to 1 L (correspond to test concentration of 1 mg/L).
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain/clone: Daphnia magna STRAUS, clone 5
- Age at study initiation (mean and range, SD): < 24hours old
- Valve height at study initiation, for shell deposition study (mean and range, SD):
- Peripheral shell growth removed prior to test initiation:
- Method of breeding:
- Source: The D. magna clone used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978 and from this date on was cultured and bred continuously at the test facility.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 2.20 – 3.20 mmol/L
- Test temperature:
- 20 ± 2°C.
- pH:
- 7.5 – 8.5
- Dissolved oxygen:
- > 3 mg/L O2
- Conductivity:
- 550 - 650 μS/cm
- Nominal and measured concentrations:
- 0 (negative control), 1, 10, 100 mg/L as nominal concentrations based on the test substance mass.
- Details on test conditions:
- TEST SYSTEM
- Material, size, headspace, fill volume: 10 mL test solution volume
- Volume of solution: 10 mL test solution volume
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic fresh water (Elendt M4)
- Intervals of water quality measurement: Throughout the test, the appearance of the test solutions and dissolution behavior of the test substance was observed and recorded daily. The chemical and physical parameters of the test medium (total hardness, acid capacity, pH, TOC and conductivity) were determined after aeration and prior to use in the test. The pH and dissolved oxygen in the test solutions are recorded at the start and end of the exposure period. Test temperature was maintained at approx. 20 ± 2°C. The pH and the oxygen content range between 8.0-8.1 and 8.3-8.8 mg/L during the exposure.
DATA EVALUATION
This study was designed to provide a qualitative estimate of toxicity. The study design was not sufficient to provide a reliable quantitative toxicity endpoint. The ECx ranges are estimated based on expert judgment in accordance with test guideline recommendations. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 - < 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The water parameters oxygen content and pH were recorded at start and end of the exposure period and within the ranges (pH 6-9, > 3 mg/L O2) required by the OECD Guideline for Testing of Chemicals No. 202, Daphnia sp., Acute Immobilisation Test.
All test assays were visibly clear and colorless after preparation and remained so after 24 and 48 hours of exposure in the test. No undissolved test substance was visible and there were no other remarkable observations.
Test validity criteria: This test was fully compliant with all the following validity criteria required by the corresponding test guidelines and is considered valid.
- < 10% immobilisation in the control
- O2 concentration > 3 mg/L in control and test vessels
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance is acutely harmful for aquatic invertebrates.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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