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EC number: 812-745-6 | CAS number: 205041-15-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 31 March 2020 to 29 June 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13 April 2004
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 12.50, 25, 50, 100 and 200 mg/L
- Sampling method: After 0 h and 48 h exposure, two replicates of 6 ml each were sampled. Per replicate 1.5 ml from each test vessel were pooled to obtain 6 mL in total. To each sample 6 mL Acetonitrile was added. The samples were filled into glass screw-cap vials and are stored in the freezer (≤ -18 °C). - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The stock solution was prepared by adding 200 mg test item to 1000 mL test medium (OECD 202 test medium) and stirring on a magnetic stirrer.
- Controls: negative control - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea, Daphnia magna Strauß
- Age at study initiation: 0.25 - 23.0 h
- Method of breeding: The daphnids are cultured at 20 ± 2 °C with 16 h light : 8 h dark and placed into fresh Elendt M4 medium twice a week. They are kept at approx. 10 animals / 200 mL and fed on workdays with Desmodesmus subspicatus algae corresponding to 0.1 mg C × daphnid-1 × day-1.
- Source: Federal Environment Agency, Department IV 2.4, Berlin (2012) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20.3 - 21.6 °C
- pH:
- 7.3 - 8.0
- Dissolved oxygen:
- 8.4 - 8.5 mg/l
- Nominal and measured concentrations:
- nominal: 12.50, 25, 50, 100, 200 mg/L (measured concentrations were within 95.6 - 105.5 % of nominal concentrations)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers 50 ml, Schott, Mainz
- Volume of solution: 20
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- No. of vessels per control: 4
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16h light : 8h dark
EFFECT PARAMETERS MEASURED : immobilisation
RANGE-FINDING STUDY
- Test concentrations: 1, 10 and 100 mg/L - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 30.85 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 69.63 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities:
none reported
- Mortality of control: 0
- Other adverse effects control: none reported
- Immobilisation of control: 0 - Results with reference substance (positive control):
- Quality assurance takes place at regular intervals by testing the sensitivity of the test organisms to the reference item potassium dichromate (Honeywell Speciality Chemicals Seelze GmgH, Seelze, Germany). The recent quality testing was performed in December 2019 (GLP 1504) with EC50(24h) = 1.95 mg/L, which is in the declared range of 0.6 - 2.1 mg/L (24h) according to OECD 202.
- Reported statistics and error estimates:
- The effect occurrence after 24 h and 48 h exposure was calculated. With the statistical software ToxRat Professional 3.3.0 (ToxRat Solutions GmbH, Alsdorf, Germany), ECx and LOEC/NOEC were determined.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Effects were observed at the lowest test concentration used in the study, therefore, it can be concluded, that N1,N3-diallylpropane-1,3-diamine dihydrochloride already has toxic effects on Daphnia magna at concentrations below 12.50 mg/L.
The 48-hr EC50 is 30.85 mg/L. - Executive summary:
The 48-hr acute toxicity of N1,N3-diallylpropane-1,3-diamine dihydrochloride to Daphnia magna Strauß was studied under static conditions. Test species were exposed to N1,N3-diallylpropane-1,3-diamine dihydrochloride at nominal concentrations of 0, 12.50, 25, 50, 100 and 200 mg/L for 48 hr. Immobilisation was observed at all concentrations. The 48-hour EC50 was 30.85 mg/L. The 48-hr NOEC based on immobilization was < 12.50 mg/L.
This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.
Results Synopsis
Test Organism Age: 0.25 - 23 h
Test Type: static
NOEC: < 12.5 mg/L
EC50: 30.85 mg/L 95% C.I.: 22.73 to 40.27 mg/L
Endpoint(s) Effected: Immobility
Reference
Table 1: Effect concentrations
Lowest/No Observed) Effect Concentration |
Nominal test item concentration (mg/L) |
|
95 % Confidence Limits (CL) |
24h |
48h |
EC50 |
69.63 |
30.85 |
CL |
46.08 – 117.9 |
22.73 – 40.27 |
EC10 |
9.84 |
10.36 |
CL |
2.32 – 18.43 |
5.07 – 15.32 |
LOEC |
≤12.50 |
≤12.50 |
NOEC |
<12.50 |
< 12.50 |
Table 2: Analytically measured test item concentrations after 0h and 48h exposure
Nominal test item concentration (mg/L) |
Sample |
Sampling time [h] |
Test item concentration (mg/L) |
Recovery [%] nominal |
Mean recovery |
NC |
1527-NC1-0h |
0 |
< LOC* |
-- |
-- |
1527-NC1-48h |
48 |
< LOC |
-- |
||
12.5 |
1527-A1-0h |
0 |
12.2 |
97.6 |
96.8 |
1527-A1-48h |
48 |
12.0 |
96.0 |
||
25.5 |
1527-B1-0h |
0 |
25.8 |
103.2 |
100.4 |
1527-B1-48h |
48 |
24.4 |
97.6 |
||
50.5 |
1527-C1-0h |
0 |
51.7 |
103.4 |
99.5 |
1527-C1-48h |
48 |
47.8 |
95.6 |
||
100.0 |
1527-D1-0h |
0 |
101 |
101.0 |
103.5 |
1527-D1-48h |
48 |
106 |
106.0 |
||
200.0 |
1527-E1-0h |
0 |
202 |
101.0 |
103.3 |
1527-E1-48h |
48 |
211 |
105.5 |
* LOC = Limit of Calibration (4 mg/L)
Table 3: Immobility after 24h and 48h exposure
Nominal test item concentration (mg/L) |
Daphnia introduced |
Immobile daphnia |
Immobility [%] |
|||||||||||
Sampling time |
0h |
24h |
48h |
24h |
48h |
|||||||||
Replicates |
1 |
2 |
3 |
4 |
1 |
2 |
3 |
4 |
1 |
2 |
3 |
4 |
||
NC |
5 |
5 |
5 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
12.5 |
5 |
5 |
5 |
5 |
1 |
1 |
1 |
0 |
1 |
1 |
1 |
1 |
15 |
20 |
25.0 |
5 |
5 |
5 |
5 |
1 |
1 |
1 |
0 |
2 |
1 |
1 |
2 |
15 |
30 |
50 |
5 |
5 |
5 |
5 |
3 |
2 |
1 |
4 |
3 |
4 |
3 |
5 |
50 |
75 |
100 |
5 |
5 |
5 |
5 |
2 |
4 |
3 |
4 |
4 |
5 |
4 |
5 |
65 |
90 |
200 |
5 |
5 |
5 |
5 |
4 |
4 |
4 |
2 |
5 |
5 |
5 |
5 |
70 |
100 |
Description of key information
In a single key study on the acute toxicity of N1,N3-diallylpropane-1,3-diamine dihydrochloride to Daphnia magna according to OECD guideline 202, an 48-hr EC50 of 30.85 mg/L was reported. The test was conducted under static conditions and fulfilled all criteria for a valid test.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 30.85 mg/L
Additional information
Daphnia magna were exposed to N1,N3-diallylpropane-1,3-diamine dihydrochloride at nominal concentrations of 0, 12.50, 25, 50, 100 and 200 mg/L for 48 hr. Immobilisation was observed at all concentrations. The 48-hr EC50 was 30.85 mg/L. The 48-hr NOEC based on immobilization was < 12.50 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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