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EC number: 202-739-6 | CAS number: 99-20-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 09. May - 26. Sept. 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Name TREHALOSE, ANHYDROUS
Batch no. 160805
Appearance White crystalline powder
Composition Trehalose: 100 % (dry basis)
The test item was stored in a closed vessel, dark and dry at room temperature 20 ± 5°C. - Analytical monitoring:
- yes
- Details on sampling:
- no specific sampling. Dilution Factor 2.5
- Vehicle:
- no
- Details on test solutions:
- A solution containing 100.4 mg/L test item in dilution water was prepared and used for the
test - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: Berlin
- Justification for species other than prescribed by test guideline:
- Source: Umweltbundesamt Berlin
- Age of parental stock (mean and range, SD): In house breeding since 27. September 2007
- Age of animals for test: between 0 and 24 hours
- Feeding during test none
ACCLIMATION
- Acclimation period: not applicable - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- according to Guideline
- Post exposure observation period:
- no
- Hardness:
- 250 mg CaCO3 / L
- Test temperature:
- 20.3 - 21.8 °C
- pH:
- 7.8
- Dissolved oxygen:
- 8.7-8.8 mg/L
- Salinity:
- not applicable
- Conductivity:
- not stated
- Nominal and measured concentrations:
- nominal: 100 mg/L
Measured at t = 0h: 93.9 mg/L
Measured at t = 48 h = 98.1 mg/L - Details on test conditions:
- Date of performance 12. Jun. - 14. Jun. 2018
Treatments 100 mg/L (nominal)
The concentrations to be tested are based on the result of
a non-GLP pre-test.
Temperature 20.3 - 21.8 °C
Duration 48 hours
Observation times 24 and 48 hours
Medium renewal none
Test vessels glass beakers, nominal volume 50 mL, tall shape
Replicates (Treatments) 4 vessels, each containing 20 ± 5 mL test solution and 5
Daphnia
Replicates (Blank control) 4 vessels, each containing 20 ± 5 mL dilution water and 5
Daphnia - Reference substance (positive control):
- yes
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- No observations were made which might cause doubts concerning the validity of the study
outcome. All validity criteria were met. - Results with reference substance (positive control):
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a
current reference study (201714R201). The 24h-EC50i value was determined as 1.9 mg/L.
The value was within the requested range for the 24h-EC50 between 0.6 - 2.1 mg/L of potassium
dichromate K2Cr2O7 as specified in the OECD guideline. Since the fits showed
sufficient statistical correspondence of the data with the dose-response-equation, this reference
study can be considered valid. - Reported statistics and error estimates:
- no statistics reported
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions, Trehalose did not show any effects on Daphnia magna and following toxicological dose discriptor is derived:
48h-NOEC ≥ 100 mg/L - Executive summary:
One valid experiment was performed.
The study was performed as a limit test using a concentration at 100 mg/L. For the test
concentration and the blank control, 20 Daphnia were exposed to the test item for 48
hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted.
No toxicity was observed and none of the animals was immobilised in the blank control.
Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a
current reference study to assure that the test conditions are reliable.
At the beginning and at the end of the test, the content of the test item in the test solutions
was determined using HPLC-MS-determination. The concentrations determined at the
start of the test were 94% of the nominal concentration. At the end of the test the determined
concentrations were 98% of the nominal concentration. Therefore, the determination
of the biological results was based on the nominal concentration.
The following results were determined for the test item TREHALOSE, ANHYDROUS (species:
Daphnia magna).
48h-NOEC ≥ 100 mg/L
48h-LOEC > 100 mg/L
24h-EC50 > 100 mg/L
48h-EC50 > 100 mg/L
Reference
Description of key information
one study on toxicity against Daphnia Magna available.
No effects of the substance were observed hence no LC50 value is given.
Key value for chemical safety assessment
Additional information
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