Titanium 2,2',2''-nitrilotrisethanolate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

RA

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: not specified

Principles of method if other than guideline:

not specified

GLP compliance:

not specified

Test organisms (species):

Pimephales promelas

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1 170 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Justification for type of information:

Read across

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: APHA (1971). Standard Methods for the Examination of Water and Wastewater: No 231. American Public Health Association Inc., New York, USA.

GLP compliance:

no

Analytical monitoring:

yes

Details on sampling:

Concentration control: - determination of the test concentrations before and after the test (TOC analyzer or GC analysis)

Vehicle:

no

Test organisms (species):

Carassius auratus

Details on test organisms:

- goldfish (Carassius auratus)
- average length: 6.2 ± 0.7 cm
- average weight: 3.3 ± 1.0 g

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Test temperature:

20±1 °C

pH:

pH 9.9, test solution not neutralised

Dissolved oxygen:

>= 4 mg/L

Nominal and measured concentrations:

nominal: not specified, but a series of concentration levels was tested

Details on test conditions:

TEST SYSTEM
- Test vessel: all-glass aquaria (42 x 28 x 28 cm)
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: test volume: 25 L
- Aeration: slight, continuous (minimum oxygen content: 4 mg/L)
- No. of organisms per vessel: 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: local tap water (chemical analysis (in mg/L): chloride = 65, nitrite = 0, nitrite = 4, sulfate = 35, phosphate = 0.15, bicarbonate = 25, silicate = 25, ammonium = 0, Fe = 0.05, Mn = 0, Ca2+ = 100, Mg2+ = 8, alkali as Na+ = 30; pH = 7)

OTHER TEST CONDITIONS
- Adjustment of pH: no

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 5 000 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: interpolation of medium tolerance limits (TLm ^= LC50) by interpolation by plotting the logarithm of the concentration versus the percentage mortality

Reference 3

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

Read across

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: German Industrial Standard DIN 38412, L 15 (draft)

Deviations:

not specified

Principles of method if other than guideline:

German Industrial Standard DIN 38412, L 15 (draft): Anonymus (1976). Deutsche Einheitsverfahren zur Wasser- Abwasser- und Schlamm-Untersuchung, L 15: Fischtest (Bestimmung der Wirkung von Wasserinhaltsstoffen auf Fische) - Vorabdruck. (German Standard Methods for the Examination of Water, Wastewater and Sludge, L15: Fish Test (Determination of the Effect of Substances in Water on Fish) - Preprint). Vom Wasser 46: 291-295

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Leuciscus idus

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

220-320 mg/L as CaCO3

Test temperature:

20 °C

pH:

The test solutions were not neutralized.

Dissolved oxygen:

continuously aerated

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Results of two tests according to the German National Standard DIN 38 412, part 15; one performed at the LWA of North-Rhine Westfalia, the other at the WaBoLu Institute in Berlin.

Validity criteria fulfilled:

yes

Description of key information

Testing on the aquatic vertebrates was considered not necessary as alternatives to testing were available and they demonstrated to be sufficient to fulfil the legal requirements related to Annex VIII, Section 9.1.3 of REACH. Therefore, the general rules for adaptation 1.2 of Annex XI of REACH regulation are met and applied. The results obtained from the three publications available from were in the EC50 range 1000 -5000 mg/l. the EC50 of 1170 mg/l has been chosen as key for the CSA.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 170 mg/L