p-vinylphenol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

07-12-2017 to 15-01-2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

Qualifier:

according to guideline

Guideline:

other: Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Maruzen Petrochemical Co, LTD. LOT: 7706RXD
- Expiration date of the lot/batch: No Data
- Purity test date: No Data

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: The test sampe was stored by freezing in a dark storage place.
- Stability under test conditions: No Data
- Solubility and stability of the test substance in the solvent/vehicle: The infrared spectrum of the test item after the completion on the experiment was the same as before the start of the experiment, indicating that the test item was stable under the storage condition.
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:
- Preliminary purification step (if any):
- Final dilution of a dissolved solid, stock liquid or gel:
- Final preparation of a solid:

FORM AS APPLIED IN THE TEST (if different from that of starting material)

TYPE OF BIOCIDE/PESTICIDE FORMULATION (if applicable)

OTHER SPECIFICS:

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Medaka
- Strain:
- Source: CERI Kurume
- Age at study initiation (mean and range, SD): three month old
- Length at study initiation (length definition, mean, range and SD): 2.0 (+/- 1cm) cm
- Weight at study initiation (mean and range, SD):
- Method of breeding: In Laboratory production
- Maintenance of the brood fish:

ACCLIMATION
- Acclimation period: 26 days (15.11.2017 - 11.12.2017)
- Acclimation conditions (same as test or not):
- Type and amount of food during acclimation: Feed of Medaka fry for education. amount corresponding to 3% of body weight was fed every day.
- Feeding frequency during acclimation: Daily
- Health during acclimation (any mortality observed): 0% mortality

QUARANTINE (wild caught)
- Duration:
- Health/mortality:

FEEDING DURING TEST
- Food type:
- Amount:
- Frequency:

Test type:

semi-static

Water media type:

other: Dechlorinated tap water

Total exposure duration:

96 h

Post exposure observation period:

Mortality and visable abnormality were observed at 3, 24, 48, 72 and 96 hours after the start of exposure.

Test temperature:

24 (+/-1) °C.

pH:

No adjusment

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

12.1 mg/L

Nominal / measured:

not specified

Conc. based on:

not specified

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities:
- Observations on body length and weight: Length - 2.4 (+/- 1) cm Body Weight - 0.14 (+/- 0.01) g.
- Other biological observations:
- Mortality of control: No mortality in control
- Other adverse effects control:
- Abnormal responses:
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:
- Effect concentrations exceeding solubility of substance in test medium:

Conclusions:

The study found that 96 -hour LC50 was 12.1 mg/L.

Description of key information

The study found that 96 hour LC50 was 12.1 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

12.1 mg/L