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EC number: 256-233-5 | CAS number: 45320-65-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
Refer to attached document - Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 0.108 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr. (dissolved fraction)
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.108 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr. (dissolved fraction)
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 0.108 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr. (dissolved fraction)
- Basis for effect:
- mortality
- Conclusions:
- N, N’-[(methylimino)bis (trimethylene)]bis(oleamide) caused no effect to Daphnia magna up to the limit of solubility based on information from the related read-across substance N,N’[(methylimino)bis(trimethylene)]bis(stearamide).
Reference
Description of key information
N, N’-[(methylimino)bis (trimethylene)]bis(oleamide) caused no effect to Daphnia magna up to the limit of solubility based on information from the related read-across substance N,N’[(methylimino)bis(trimethylene)]bis(stearamide).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.108 mg/L
Additional information
Available information from an OECD 202 guideline study with the test substance N,N’[(methylimino)bis(trimethylene)]bis(stearamide) were used to evaluate short-term toxicity to aquatic invertebrates of N, N’-[(methylimino)bis(trimethylene)]bis(oleamide).
In the acute immobilization test with Daphnia magna (STRAUS), the effects of the test itemN,N’‑[(methylimino)bis(trimethylene)]bis(stearamide) were determined according to OECD 202 (2004).
The test item is a yellow solid with a low water solubility of 10 - 500 µg/L.In the definitive test the solventTetrahydrofuran containing 0.1% formic acid (THF+FA) was used as it helps dissolving the test item in the test medium at the limit of the water solubility. As observed in the range finding test, the test item is insoluble / instable in the test medium and under test conditions and, therefore, the use of a solvent was necessary to prepare a defined test item concentration at the limit of the water solubility of the test item. A limit concentration of 0.100 mg/L of the test item was tested.
The study was performed over a period of 48 hours under semi-static conditions with a renewal of test solution after 24 hours as a worst case exposure. Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to the limit concentration, the solvent control and the control.
The limit concentrationwas colorless and visually clear throughout the exposure period.
The concentration of the test item N,N’-[(methylimino)bis(trimethylene)]bis(stearamide) was analytically verified via LC-MS/MS in the fresh media at the start of the exposure (0 hours and 24 hours) and in the corresponding old media and the renewal of the test solutions and at the end of the exposure (24 hours and 48 hours) in the limit concentration, the solvent control and the control.
The measured concentration of the test item in the fresh media (0 and 24 hours) was 64% and 306% of the nominal test item concentration and 101% and 69 % in the corresponding old media (24 and 48 hours). The geometric mean measured concentration is 0.108 mg/L. This concentration is considered to be the maximum solubility level of the test item in test medium under test conditions..
The effect levels given are based on the geometric mean measured concentration of the test item N,N’-[(methylimino)bis(trimethylene)]bis(stearamide):
EC10/ EC50/ EC10024 hours: > 0.108 mg/L
EC10/ EC50/ EC10048 hours: > 0.108 mg/L
The validity criteria of the test guidelines were fulfilled.
The test item N,N’‑[(methylimino)bis(trimethylene)]bis(stearamide) caused no effect to Daphnia magna up to the limit of solubility in the test medium and under the test conditions.
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