5-amino-2-chloro-N-(2,4-dimethylphenyl)benzenesulphonamide

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991: May 14 to October 8

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))

Version / remarks:

(1981)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.9 (Biodegradation: Zahn-Wellens Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

5-amino-2-chloro-N-(2,4-dimethylphenyl)benzenesulphonamide

EC Number:

275-269-2

EC Name:

5-amino-2-chloro-N-(2,4-dimethylphenyl)benzenesulphonamide

Cas Number:

71215-81-1

Molecular formula:

C14H15ClN2O2S

IUPAC Name:

5-amino-2-chloro-N-(2,4-dimethylphenyl)benzene-1-sulfonamide

Test material form:

solid

Details on test material:

Identity: ACDMA
CAS-No.: 71215-81-1
Solubility in water: slight
Storage Conditlons: At room temperature, in the dark
Safety Precautlons: Routlne hygienic procedures were sufficlent to assure personnel health and safety.

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Microorganisms (activated bacterial sludge) from a domestic waste-water sewage plant (ARA, Sissach/Switzerland)

Duration of test (contact time):

28 d

Initial test substance concentration

Based on:

not specified

Details on study design:

Test Medlum

The stock solutions were:

a) 8.5 g KH2PO4, 21.75 g K2HP04, 44.6 g Na2HPO4 x 12H2O, 1.7 g NH4CI dissolved up to 1000 mL bidistilled water.
b) 22.5 g MgSO4 x 7H2O dissolved up to 1000 mL bidistiIIed-water .
c) 27.5 g CaCl2 dissolved up to 1000 mL bidistillled water.
d) 0.25 g FeCl3 x 6H2O dlssolved up to 1000 mL bidlstiIled water.

Three (3) mL each of the stock solutions (a-d) were added up to 1000 mL bidistilled water (final test medium pH adjusted to 7.0).

An amount of sludge fron a domestlc waste-water sewage plant corresponding to 7.5 mg dry material for the positlve control ANILINE and 25.0 mg dry material for the test article were added to 250 mL of the final test medium.

Sanples:
Flask 1: 25O mL bidistilled water and 7.3 mg test article
Flask 2: 25O ml final test medlum, 7.5 mg test article,
Flask 3: 7.4 mg test article,
Flask 4: 7.3 mg test article and 25 mg inoculun.
The pH was adJusted to pH 7.0 before the lnoculum was added.
Flask 13: 25O ml final test redlum, 25 mg ANILINE,
Flask 14: 25 mg ANILINE,
Flask 15: 24 mg ANILINE and 7.5 mg inoculum.
Flask 16: 25O mL flnal test medlum and 25 mg inoculum.

Incubatl on:
Procedure: Inoculated brown glass bottles (500 ml) were lncubated on a BSB 1000 or BSB 501 apparatus (Wissenschaftliche-Technische Werkstätten GmbH, Weilheim/FRG).
Duration: 28 days
Temperature: 25.5 - 27.5°C, Protected from light

Analyses:
Sample preparation:
At the end of the incubation period, samples of about 10 mL from the flasks treated with ACDMA were centrifuged at about 3000g for about 5 minutes and thereafter directly analysed.

T0C-analyses:
T0C-analyses were perforned with the various supernatants, using a Shimadzu 500 TOC-Anal yser.

Cal cul ati ons:
The biodegradability (%BD) was calculated accordlng to the below given formula:

%BD = BOD/(COD or ThOD) x 100%BOD

The valtdtty of the test was evaluated by calculatlon of the biodegradability of the standard compound ANILINE.

Determlnatlon of Theoretlcal Oxygen Demand (ThOD) of ANILINE:
The theoretlcal oxygen demand of ANILINE was 241 mg oxygen per 100 mg ANILINE as calculated from the known molecular weight, assuming the turnover of H into H2O, C into CO2 and N into NH3, accordtng to attachment 2, Directive 84/449 EEC, C.7.

Determlnatlon of Chemical Oxygen Demand (COD) of ACDMA:
The COD was determined according to Directlve 84/49 EEC, C.9.
It was estimated to be 244.94 mg O2/100 mg.

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

other: % biodegradation (BOD/COD)

Value:

15

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

Biodegradation (BOD/ThOD) of Aniline: 65.4% after 28 days

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Interpretation of results:

not inherently biodegradable

Conclusions:

Inherent biodegradation of ACDMA has been measured at 15% (BOD/COD) within 28 days in test performed accordinf to OECD TG 302C.

Executive summary:

Inherent biodegradation of ACDMA has been measured at 15% (BOD/COD) within 28 days in test performed accordinf to OECD TG 302C.