Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-809-6 | CAS number: 100-00-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well documented publication/study report which meets basic scientific principles
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Reference Type:
- secondary source
- Title:
- 1-CHLORO-4-NITROBENZENE CAS No: 100-00-5 SIDS Initial Assessment Report.
- Author:
- OECD
- Year:
- 2 002
- Bibliographic source:
- UNEP Publications
Materials and methods
- Principles of method if other than guideline:
- NEN 6502: Determination of chronic toxicity to Daphnia magna (1980)
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 1-chloro-4-nitrobenzene
- EC Number:
- 202-809-6
- EC Name:
- 1-chloro-4-nitrobenzene
- Cas Number:
- 100-00-5
- Molecular formula:
- C6H4ClNO2
- IUPAC Name:
- 1-chloro-4-nitrobenzene
- Details on test material:
- - Name of test material (as cited in study report): 4-chloronitrobenzene
- Analytical purity: > 98 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The daphnids were fed with 3.0E+08 cells/L Chorella pyrenoidosa
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Total exposure duration:
- 21 d
Test conditions
- Hardness:
- 200 mg/L CaCO3
- Test temperature:
- 20 °C
- pH:
- 8.4
- Details on test conditions:
- - The population growth constant (Rm) of Daphnia magna was determined under semistatic conditions over a 21 days period, using 10 daphnids per concentration and one animal per jar containing 10 mL medium.
- The test was carried out in a room illuminated 12h/day.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- 4.6 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL; 4.3-5.0
- Duration:
- 21 d
- Dose descriptor:
- other: LRCT rm
- Effect conc.:
- 1.8 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- other: population growth
- Remarks on result:
- other: LRCT = Lowest rejected concentration tested with effect (similar to LOEC); rm=intrinsic rate of natural increase
- Duration:
- 21 d
- Dose descriptor:
- other: LRCT L
- Effect conc.:
- 3.2 mg/L
- Basis for effect:
- growth
- Remarks on result:
- other: = Lowest rejected concentration tested with effect (similar to LOEC); L=mean length (L) of animals
Any other information on results incl. tables
Three chronic effects are presented.
1. Population growth:
LRCT (Rm)= Lowest rejected concentration tested which
significantly lowered the population growth constant (Rm)
after 21 days of exposure:
log LRCT(Rm) = 1.05 = 11 µmol/L.....LRCT (Rm)=1.8 mg/L
2. Body length:
LRCT(L) = Lowest rejected concentration tested that significantly lowered the mean length (L) of animals after 21 days of exposure. log LRCT(L) = 1.31 = 20 µmol/L....LRCT(L)= 3.2 mg/L
3. Lethal concentration:
LC50 (21 days) = 4.6 (95 % confidence limits: 4.3 - 5.0) mg/L In another publication, Deneer et al. (Deneer, J.W. et al., QSAR study of the toxicity of nitrobenzene derivatives towards Daphnia magna, Chlorella pyrenoidosa and Photobacterium phosphoreum, Aquatic Toxicology 15, 83-98 (1989)), two of the authors presented the first two chronic effects but reported the third to be IC50 = 21 d immobilization concentration log IC50 = 1.46 = 28 µmol/L .......IC50 = 4.5 mg/L
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)