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EC number: 700-100-0 | CAS number: 39716-58-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2055
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Pent-4-enoic acid
- EC Number:
- 209-732-7
- EC Name:
- Pent-4-enoic acid
- Cas Number:
- 591-80-0
- Molecular formula:
- C5H8O2
- IUPAC Name:
- pent-4-enoic acid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- samples of all concentration levels from freshly prepared media were taken after 0 h and 72 h
samples of all concentration levels of 24 h old media were taken after 24 h and 96 h
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Common name: Danio rerio (zebrafish)
average body weight at test start: 0.13 g
average body lenght at test start: 2.57 cm
fish density less than 1 g per litre test solution
Feeding during test: Food was provided 3 times per week. The amount of food was 4 % of the fish body weight per feeding day. The test fish were
not fed 24 h before test start.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- 64 - 77 mg CaCO3/L
- Test temperature:
- 23 +/- 2 °C
- pH:
- 6.0 - 8.5
- Dissolved oxygen:
- not less than 60 % of air saturation value
- Nominal and measured concentrations:
- nominal concentration: 6.25 - 12.5 - 25 - 50 - 100 mg/L
measured concentrations: recovery rates of of the test item were > 80 % in all samples in fresh and 24 h old media - Details on test conditions:
- Test duration: 96 h
Test vessel: Glass-aquaria loosely covered by glass tops were used.
Test volume: About 10 L per vessel.
Aeration: Gentle aeration was provided.
Test water: Same as for holding.
Number of fish: 7 zebrafish were used per concentration and control.
Loading, length of fish: Fish density in the tanks was than less than 1 g fish per litre test solution.
Average body weight at the test start: 0.13 g
Average body length at the test start: 2.57 cm
Replicates: One replicate per test concentration and control
Introduction of fish: Fish were introduced randomly to individual replicates.
Feeding of test fish: The fish were not fed during the test.
Water temperature: 23 ± 2 °C
Dissolved oxygen concentration: Not less than 60 % of air saturation value.
Light intensity: 0.1 - 10 μmol photons · m-2 · s-1
Photoperiod: A natural photoperiod occurred during the test. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 70.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL 66.0 - 75.8 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Reported statistics and error estimates:
- LC50-values and confidence intervals were calculated by probit analyses. Probit values according to WEBER (1986) were used. Calculation of the confidence intervals for LC50 was carried out according to Breitig und Tümpling (1982).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study 4-Pentenoic acid was found to be toxic to Zebrafish after 96 h at a nominal test item concentration of 100 mg/L and higher.
The LC50 (96 hours) was 70.7 (66.0 - 75.8) mg/L. The NOEC after 96 hours was 50 mg/L (nominal test item concentration).
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