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EC number: 944-090-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- From January 18, 2007 to December 06, 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: PARCOM Marine Fish, Acute Toxicity Method
- Version / remarks:
- 1995
- Deviations:
- no
- Principles of method if other than guideline:
- Test species Scophthalmus maximus is a standard species for OSPAR HMCS since it is a common species in the North Atlantic, Baltic Sea and Mediterranean Sea.
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: p-Toluene sulphonamido caproic acid morpholine salt at a nominal concentration of 7.51 mg/L was diluted in artificial seawater (Prodac Ocean Fish Marine Salt), in which it was soluble and reportedly dispersed well. Each vessel contained a 10 litre volume of test or control media
- Controls: Artificial seawater - Test organisms (species):
- other: Scophthalmus maximus
- Details on test organisms:
- TEST ORGANISM
- Common name: Juvenile turbot (Scophthalmus maximus)
- Source: Manx Mariculture Ltd, Castletown, Isle of Man
ACCLIMATION
- Acclimation conditions: Animals were maintained as detailed in STL Runcorn SOP I.16, with stock juvenile turbot held in artificial seawater (STL Runcorn SOP III.12) kept at 15 +/- 2 °C by a chiller unit and aerated via air diffusers attached to the laboratory air supply system
- Type and amount of food during acclimation: Animals were fed as detailed in STL Runcorn SOP I.16
- Feeding frequency during acclimation: Detailed in STL Runcorn SOP I.16, with feeding stopped when juvenile turbot were transferred to test tanks 24 hours before test start
- Health during acclimation: Juvenile turbot were received on September 26, 2007 free of malformations and in good heath (Batch No. SM07002)
QUARANTINE
- Not applicable (Not wild caught)
FEEDING DURING TEST
- No
OTHER
- The test organism is the species recommended by the Paris Commission for studies on the effects of test chemicals on marine vertebrates - Test type:
- semi-static
- Water media type:
- saltwater
- Remarks:
- Artificial seawater (Prodac Ocean Fish Marine Salt)
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- Observations were made at 0, 24, 48, 72 and 96 hours to determine toxicity after exposure to the test substance
- Test temperature:
- 12.1 +/- 0.7 °C
- pH:
- 7.92 +/- 0.2 in the control and 7.96 +/- 0.1 in treated media
- Dissolved oxygen:
- 100.0 %
- Salinity:
- 31.6 +/- 0.4 % in the control and 31.9 +/- 0.3 % in treated media
- Nominal and measured concentrations:
- Nominal concentrations of 0 (control) and 7.51 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Not specified
- Material, size, headspace, fill volume: 10 litre volume of treated or control media
- Type of flow-through: Semi-static
- Renewal rate of test solution: Replacement of test and control media after 48 hours
- No. of organisms per vessel: 7 juvenile turbot
- No. of vessels per concentration: 1
- No. of vessels per control: 1
- No. of vessels per vehicle control: N/A
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Artificial seawater (Prodac Ocean Fish Marine Salt)
- Alkalinity: 7.72 - 8.21 pH
- Culture medium different from test medium: Animals were maintained as detailed in STL Runcorn SOP I.16 and held in artificial seawater (STL Runcorn SOP III.12). The test tanks contained artificial seawater of Prodac Ocean Fish Marine Salt
- Intervals of water quality measurement: Dissolved oxygen, pH, temperature, and salinity were recorded at 24 hour intervals
EFFECT PARAMETERS MEASURED: Mortality, i.e. cessation of movement and failure to respond to tactile stimulation. Observations were made at 0, 24, 48, 72 and 96 hours
TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: A test was performed using the EC50 observed during the toxicity testing on algae to determine if fish is more sensitive to the test substance than Skeletonema costatum.
- Test concentrations: 0 (control) and 7.51 mg/L of p-toluene sulphonamido caproic acid morpholine salt - Reference substance (positive control):
- not specified
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 7.51 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 0% mortality
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 7.51 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 0% mortality
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 7.51 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 0% mortality
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 7.51 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 0% mortality
- Sublethal observations / clinical signs:
After 96 hours exposure to p-toluene sulphonamido caproic acid morpholine salt, LC50 and percentage mortality in juvenile turbot were determined to be >7.51 mg/L and 0% respectively.
Mortality data for juvenile turbot exposed to control and test media containing p-toluene sulphonamido caproic acid morpholine salt:
Test Material Concentration (mg/L) Exposure Period (hours) Mortality at Test End (%) 0 24 48 72 96 0 Alive 7 7 7 7 7 - Dead 0 0 0 0 0 0 7.51 Alive 7 7 7 7 7 - Dead 0 0 0 0 0
0 - Validity criteria fulfilled:
- yes
- Conclusions:
- Following a short-term (96 hour) exposure of juvenile turbot to p-toluene sulphonamido caproic acid morpholine salt in artificial seawater, the LC50 and percentage mortality of the substance was reported as >7.51 mg/L and 0 %, respectively.
- Executive summary:
The acute toxicity of p-toluene sulphonamido caproic acid morpholine salt to the fish Scophthalmus maximus (juvenile turbot) was determined in accordance to the OECD Guideline 203 (Fish, Acute Toxicty Test), with no reported deviation. The test substance was dispersed within artificial seawater media at a nominal concentration of 0 (control) and 7.51 mg/L and monitored under semi-static conditions for 96 hours. After 24, 48, 72, and 96 hours of exposure to p-toluene sulphonamido caproic acid morpholine salt, the percentage mortality in the treated vessel was found to be 0 % and an LC50 of >7.51 mg/L was subsequently reported. Similarly, all juvenile turbot in the control vessel remained mobile and responded to tactile stimulation by the test end.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 7 July 2008 to 11 July 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: OSPAR / PARCOM Protocols on Methods for the Testing of Chemicals Used in the Offshore Industry 2006
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A nominal 1,000 mg/L solution was prepared in dilulation water, shaken vigorously and allowed to stand for one hour.
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): 0 and 15 mg/L (limit test)
- Evidence of undissolved material: A clear colourless solution was obtained. - Test organisms (species):
- Cyprinodon variegatus
- Details on test organisms:
- TEST ORGANISM
- Common name: Sheepshead Minnow
- Source: Opus Plus Ltd, Flotta, Stromness
- Length at study initiation: 17.7 mm (mean)
- Weight at study initiation: 0.07 g (mean)
ACCLIMATION
- Acclimation period: 5 days
- Acclimation conditions: same as test
- Type and amount of food during acclimation: Animals were fed daily using Tetramin Marine Flake Food at a rate of approximately 2% body weight per day until 24 hours before test initiation.
- Health during acclimation: No mortality observed - Test type:
- semi-static
- Water media type:
- saltwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Test temperature:
- 21.5 to 22.5°C
- pH:
- 7.9 - 8.1
- Dissolved oxygen:
- 95 to 100% ASV
- Salinity:
- 34%
- Nominal and measured concentrations:
- 0 and 15 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 12 L volume aquaria
- Type: open
- Renewal rate of test solution: Test solutions replaced after 48 hours.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): One replicate
- No. of vessels per control (replicates): One replicate
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light / 8 hours dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Test concentrations: 0 and 15 mg/L
- Results used to determine the conditions for the definitive study: Results from previous short-term toxicity testing on algae and aquatic invertebrates - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 15 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 15 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: None observed
- Observations on body length and weight: No abnormalities reported
- Mortality of control: None observed
- Other adverse effects control: None reported
- Abnormal responses: None reported - Validity criteria fulfilled:
- yes
- Conclusions:
- Following a short-term (96 hour) exposure of Sheepshead Minnow to p-toluene sulphonamidocaproic acid morpholine salt in artificial seawater, the LC50 and percentage mortality of the substance was reported as > 15 mg/L and 0 %, respectively.
- Executive summary:
The acute toxicity of p-toluene sulphonamidocaproic acid morpholine salt to the fish Cyprinodon variegatus (Sheepshead Minnow) was determined in accordance to the OECD Guideline 203 (Fish, Acute Toxicty Test), with no reported deviation. The test substance was dispersed within artificial seawater media at a nominal concentration of 0 (control) and 15 mg/L and monitored under semi-static conditions for 96 hours. After 24, 48, 72, and 96 hours of exposure to p-toluene sulphonamidocaproic acid morpholine salt, the percentage mortality in the treated vessel was found to be 0% and an LC50 > 15 mg/L was subsequently reported. No abnormal behaviour was reported.
Referenceopen allclose all
Description of key information
The acute toxicity of p-toluene sulphonamido caproic acid morpholine salt to marine water fish was determined in two studies conducted in accordance with the OECD Guideline 203 (Fish, Acute Toxicty Test), with no reported deviation.
Both studies were limit tests using the lowest EC50/LC50 determined in the short-term toxicity test to algae and aquatic invertebrates. The highest concentration tested was 7.51 mg/L during the first study and 15 mg/L during the second study.
No animal died or displayed abnormal behaviour as a result to the exposure to the test substance at both 7.51 mg/L and 15 mg/L.
Based on these results it is concluded that the LC50 for the registered substance should be reported as > 15 mg/L.
Key value for chemical safety assessment
Marine water fish
Marine water fish
- Effect concentration:
- 15 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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