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EC number: 816-307-5 | CAS number: 45738-97-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- genetic toxicity in vitro, other
- Remarks:
- Allium chromosome aberration test
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1982
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Chromosome aberration assays in Allium: A report of the US Environmental Protection Agency Gene-Tox Program
- Author:
- Grant WF
- Year:
- 1 982
- Bibliographic source:
- Mutation Research 99 (1982) 273-291
- Report date:
- 1982
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Breaks, exchanges and gaps involving subchromatid, chromatid, and chromosomate aberrations and rearrangements can be seen in Allium cepa, whose morphological characteristics are established and can be compared to determine the effect of a potentially mutagenic chemical.
- GLP compliance:
- not specified
- Type of assay:
- other: Allium chromosome aberration assay
Test material
- Reference substance name:
- Purin-6(1H)-one
- EC Number:
- 200-697-3
- EC Name:
- Purin-6(1H)-one
- Cas Number:
- 68-94-0
- Molecular formula:
- C5H4N4O
- IUPAC Name:
- hypoxanthine
- Test material form:
- solid: particulate/powder
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: Allium cepa
- Test concentrations with justification for top dose:
- no test concentration specified
- Vehicle / solvent:
- Vehicle not specified
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- ethylmethanesulphonate
- Details on test system and experimental conditions:
- GENERAL PROCEDURE
Young onion bulbs of 10-30 g are prepared so that the apices of the root primordia are exposed. The bulbs are cultured in tap water (submerged 1/4 the depth of the bulb) aerated by continuous bubbling in the dark 2-5 days at 20°C. The mitotic cycle and period of DNA is measured.
When the root tips are 1-5 cm in length, the bulbs are transferred to a similar vessel containing the test substance in solution. Treatment times vary between 2 to 24 hours.
Chromosome aberrations aberrations are scored at different stages in the cell cycle, so recovery period prior to fixation includes one complete cell cycle.
After the recovery period, the root tips are fixed immediately or pretreated with colchicine or 8-hydroxyquinoline prior to fixation for accumulation of metaphases. Then, they are fixed in 95% alcohol-alcial acetic acid from 30 min to 24 hours, stored in 70% ethanol, and then stained at any point using aceto-carmine, aceto-orcein, or Feulgen reagent procedures.
To prepare slides, samples are treated with 5% pectinase for 1-3 hours. On each slide, the meristematic region is pressed in a drop of 45% acetic acid and mounted with a coverslip and a temporary seal. - Evaluation criteria:
- The number of specifci type of aberration per 100 cells or the total number of aberrations per 100 cells.
Results and discussion
Test results
- Key result
- Species / strain:
- other: Allium cepa
- Metabolic activation:
- not specified
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
Applicant's summary and conclusion
- Conclusions:
- The test substance, Hypoxanthine, tested negative in the Allium chromosomal aberration test.
- Executive summary:
The test substance, Hypoxanthine, tested negative in the Allium chromosomal aberration test.
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