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EC number: 239-825-8 | CAS number: 15733-22-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11 Aug 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Ministerium für Umwelt und Naturschutz, Landwirtschaft und Verbraucherschutz des Landes Nordrhein-Westfalen
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct weighting
- Differential loading: yes
- Controls: blank control
- Eluate: no - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture:
no
- Name and location of sewage treatment plant where inoculum was collected: STP, Cologne-Stammheim, Germany (aeration tank)
- Method of cultivation: Aeration of the activated sludge at 20 °C ± 2 °C, daily feed with synthetic medium.
- Preparation of inoculum for exposure: First, the sludge is settled and supernatant is decanted. After centrifuging (15 min at 4000 rpm and 20°C) the supernatant is decanted again. 1 g of wet sludge is dried in order to calculate the amount of wet sludge to achieve a concentration of activated sludge of 3 g/L (dry wet) suspended solids. The calculated amount of sludge is first dissolved in synthetic medium and then filled up to a defined end volume with de-ionized water.
- Pretreatment: none
- Concentration of Inoculum: 3 g/L (dry weight). - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20.7 - 20.9 °C
- pH:
- 7.7 - 7.9
- Dissolved oxygen:
- See "Any other information on results incl. tables"
- Nominal and measured concentrations:
- nominal: control, 10, 18, 32, 56 and 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 mL glass Erlenmeyer flasks
- Aeration: yes
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Sludge concentration (weight of dry solids per volume): 800 mg/L suspended solids
TEST MEDIUM / WATER PARAMETERS
- Preparation of test medium: according to guideline
OTHER TEST CONDITIONS
- Adjustment of pH: yes, adjusted to pH 7±0.5 using HCl
EFFECT PARAMETERS MEASURED: respiration inhibition after 3 h of incubation
TEST CONCENTRATIONS
- Range-finding test : 10, 100 and 1000 mg/L
- Results used to determine the conditions for the definitive study: The range-finding test revealed inhibition rates of 20.1%, 74.2% and 100% for test substances concentrations of 10, 10 and 100 mg/L, respectively. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 5.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: respiration rate
- Remarks:
- 95% CL: 1.6 - 10.2 mg/L
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 41.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: respiration rate
- Remarks:
- 95% CL: 30.8 - 58.7 mg/L
- Details on results:
- - Effect concentrations exceeding solubility of substance in test medium:
no
- Blank controls oxygen uptake rate: no physico-chemical oxygen consumption was observed - Results with reference substance (positive control):
- - Results with reference substance valid? yes, the EC50 of 3,5-dichlorophenol is in the accepted range 5 to 30 mg/L
- EC50 (3 h): 7.2 mg/L (95% CI: 3.8 - 13.2 mg/L) - Reported statistics and error estimates:
- Inhibitory effect of the test item: Percentage of the mean of the respiration rates of two controls. EC50 values: Determined by probit analysis of the respiration rates at different test substance concentrations.
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
- Conclusions:
- EC 50 (3 h) = 41.4 mg/L
EC 10 (3 h) = 5.7 mg/L - Executive summary:
The effect of p-chloro-m-cresol on aquatic micro-organism was investigated by Neuhahn (2008) in accordance with the OECD guideline 209 and EC Method C.11. P-chloro-m-cresol showed inhibitory effects on activated sludge. 50% inhibition of microbial activity was determined at 41.4 mg/L. The EC10 was determimed at 5.7 mg/L.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Refer to analogue justification document provided in IUCLID section 13.
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 5.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: respiration rate
- Remarks:
- 95% CL: 1.6 - 10.2 mg/L
- Remarks on result:
- other:
- Remarks:
- source, CAS 59-50-7, key rel 1, Neuhahn 2008
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 41.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: respiration rate
- Remarks:
- 95% CL: 30.8 - 58.7 mg/L
- Remarks on result:
- other:
- Remarks:
- source, CAS 59-50-7, key rel 1, Neuhahn 2008
Referenceopen allclose all
Table 1: Main test (test substance CMK): Oxygen content, temperature and pH values during exposure phase
|
Test item concen-tration [mg/L] |
O2 start [mg O2/L] |
O2 end [mg O2/L] |
Time (start-end) [min.] |
Temp. [°C] |
pH |
Test item |
10 |
5.7 |
2.6 |
7 |
20.6 |
7.7 |
18 |
5.5 |
2.8 |
7 |
20.5 |
7.7 |
|
32 |
5.8 |
2.7 |
9 |
20.7 |
7.7 |
|
56 |
5.9 |
3.9 |
8 |
20.8 |
7.6 |
|
100 |
6.2 |
5.0 |
9 |
20.9 |
7.5 |
|
Control 1 |
|
5.7 |
2.8 |
5 |
21.1 |
7.7 |
Control 2 |
|
5.4 |
2.7 |
5 |
20.6 |
7.7 |
Physico-chemical oxygen consumption control * |
|
7.5 |
7.5 |
9 |
20.8 |
7.3 |
Comments: * Data obtained in the range finding test. The test item was added to about 130 mL deionized water and stirred overnight before testing (equilibration phase).
Table 2: Results of test item
Test item concen-tration [mg/L] |
Respiratory ratetest item [mg/L h] |
Phys.-chem. O2 consumption [mg/L⋅h] |
Respiratory rate - phys.-chem. O2 consumption [mg/L⋅h] |
Inhibition [%] |
10 |
26.6 |
0.0* |
26.6 |
20.9 |
18 |
23.1 |
0.0* |
23.1 |
31.1 |
32 |
20.7 |
0.0* |
20.7 |
38.5 |
56 |
15.0 |
0.0* |
15.0 |
55.4 |
100 |
8.0 |
0.0 |
8.0 |
76.2 |
Control. Mean |
33.6 |
|
||
Control 1 |
34.8 |
|
||
Control 2 |
32.4 |
|
* The physico-chemical oxygen consumption has been determined at 1000 mg/L test item concentration. As no physico-chemical oxygen consumption was observed at that test item concentration this observation also holds true for the lower test item concentrations.
Table 3: Validity criteria
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The two control respiration rates are within 15 per cent of each other. |
Respiratory rates of the 2 controls differ less than 15 % |
yes |
The EC 50 (3 hours) of 3,5-dichlorophenol is in the accepted range 5 to 30 mg/L. |
EC50 of the reference compound 3.5-Dichlorophenol is in the range 5 – 30 mg/L |
yes |
Description of key information
EC50 (3 h) = 41.4 mg/L (nominal, activated sludge microorganisms), read-across
EC10 (3 h) = 5.7 mg/L (nominal, activated sludge microorganisms), read-across
Key value for chemical safety assessment
- EC50 for microorganisms:
- 41.4 mg/L
- EC10 or NOEC for microorganisms:
- 5.7 mg/L
Additional information
No study investigating the toxicity of sodium p-chloro-m-cresolate (CAS 15733-22-9) to aquatic microorganisms is available. Therefore, in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5 a read-across to the source substance (p-chloro-m-cresol, CAS 59-50-7) which is the main transformation product of the target substance sodium p-chloro-m-cresolate (CAS 15733-22-9) is applied.
The source substance p-chloro-m-cresol is the common compound in this analogue approach and is solely responsible for the (absence of) effects. Thus, the source substance is considered a suitable representative for the evaluation of the toxicity of the target substance to aquatic organisms. The read-across approach is justified in detail within the analogue justification in IUCLID section 13.
One experimental study is available investigating the effect of p-chloro-m-cresol on aquatic microorganism (Neuhahn, 2008). The test was conducted according to OECD guideline 209 and EC Method C.11. Activated sludge microorganisms were exposed for 3 hours to nominal concentrations of 10, 18, 32, 56 and 100 mg/L. The inhibition of respiration was measured at the end of the study. P-chloro-m-cresol showed inhibitory effects on activated sludge microorganisms. 50% inhibition of microbial activity was determined at 41.4 mg/L. Therefore the EC50 (3 h) was 41.4 mg/L. The EC10 (3 h) was determined to be 5.7 mg/L.
Based on the available results from a structurally similar source substance (in accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5) which is the main transformation product of the target substance and is characterized by a similar ecotoxicological profile and comparable structure, it can be concluded that sodium p-chloro-m-cresolate has effects in the same range as the target substance.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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