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EC number: 205-593-1 | CAS number: 143-23-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- For details on endpoint specific justification please see read-across report in section 13 or find a link in cross-reference “read-across: supporting information”.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- assessment report
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 179 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- BHT
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CL: 143-231 mg/L
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 3-hour EC50 of the test item BHT amine on the activated sludge respiration was found to be 335 mg/L with 95% confidence limits from 267 to 432 mg/L.
The 3-hour EC10 was found to be 12 mg/L with 95% confidence limits from 6.4 to 18 mg/L.
The EC50 of 335 mg/L for the test item BHT amine (with 53.5% BHT) leads to a EC50 of 179 mg BHT/L - Executive summary:
The inhibitory effect of the test item BHT amine on the respiration rate of aerobic wastewater micro-organisms of activated sludge was investigated in a 3-hour respiration inhibition test according to the OECD Guideline for Testing of Chemicals, No. 209, adopted 2010.
Range-finding test
A range-finding test using the following three test item concentrations of 10 mg/L, 100 mg/L and 1000 mg/L and four blank controls was performed. As a positive control, the reference item 3,5-dichlorophenol was tested under identical test conditions at concentrations of 3.2, 10 and 32 mg/L.
The results of the blank and positive control treatments confirmed suitability of the activated sludge and the method used. The validity criteria were fulfilled.
In conformity with the guideline, since in the range-finding test the test item showed inhibitions of the respiration rate of 65, 26 and 6.4% for the test item concentrations of 1000, 100 and 10 mg/L, respectively, a definitive test was performed.
Definitive test
Based on the results of the range-finding test and in agreement with the Sponsor a definitive test with the following nominal test item concentrations was performed: 10, 32, 100, 320 and 1000 mg/L with three replicates per concentration to permit the determination of the EC50.
As a positive control, the reference item 3,5-dichlorophenol was tested under identical test conditions at concentrations of 3.2, 10 and 32 mg/L (all single replicates).
Six blank controls, containing an equal volume of activated sludge and synthetic medium but no test or reference item were included in the definitive test.
The vessels were prepared in the following order: two blank controls, the reference item group (with ascending concentrations), two further blank controls, the test item group (with ascending concentrations) and at last two blank control vessels.
The mean inhibition of the respiration rates for the tested concentrations was 8.8% at 10 mg/L, 21% at 32 mg/L, 31% at 100 mg/L, 48% at 320 mg/L and 68% at 1000 mg/L compared to the control, i.e. a clear concentration-effect relationship on the inhibition of the respiration rate was determined.
Based on this the ECX values and their 95% confidence limits were as follows:
EC10 12 mg/L, 95% confidence limits: 6.4 - 18 mg/L
EC20: 37 mg/L, 95% confidence limits: 25 - 51 mg/L
EC50: 335 mg/L, 95% confidence limits: 267 - 432 mg/L
EC80: >1000 mg/L.
The EC50 of 335 mg/L for the test item BHT amine (with 53.5% BHT) leads to a EC50 of 179 mg BHT/L.
The study used as source investigated Reaction mass of 7-azatridecane-1,13-diamine and hexamethylenediamine (EC 907 -605 -7, which contains relevant amounts of the submission substance,
7-azatridecane-1,13-diamine).The study results of the source compound were considered applicable to the target compound. Justification and applicability of the read-across approach (structural analogue) is outlined in the read-across report in section 13 or find a link in cross reference “assessment report”.
Reference
Description of key information
The inhibitory effect of the test item BHT amine (reaction mass with BHT as main constituent, at 53.5%) on the respiration rate of aerobic wastewater micro-organisms of activated sludge was investigated in a 3-hour respiration inhibition test according to the OECD Guideline for Testing of Chemicals, No. 209, adopted 2010.
Definitive test
Based on the results of the range-finding test a definitive test with the following nominal test item concentrations was performed: 10, 32, 100, 320 and 1000 mg/L with three replicates per concentration to permit the determination of the EC50. As a positive control, the reference item 3,5-dichlorophenol was tested under identical test conditions at concentrations of 3.2, 10 and 32 mg/L (all single replicates).
Six blank controls, containing an equal volume of activated sludge and synthetic medium but no test or reference item were included in the definitive test.
The vessels were prepared in the following order: two blank controls, the reference item group (with ascending concentrations), two further blank controls, the test item group (with ascending concentrations) and at last two blank control vessels.
The mean inhibition of the respiration rates for the tested concentrations was 8.8% at 10 mg/L, 21% at 32 mg/L, 31% at 100 mg/L, 48% at 320 mg/L and 68% at 1000 mg/L compared to the control, i.e. a clear concentration-effect relationship on the inhibition of the respiration rate was determined.
Based on this the ECX values and their 95% confidence limits were as follows:
EC10 12 mg/L, 95% confidence limits: 6.4 - 18 mg/L
EC20: 37 mg/L, 95% confidence limits: 25 - 51 mg/L
EC50: 335 mg/L, 95% confidence limits: 267 - 432 mg/L
EC80: >1000 mg/L.
The EC50 of 335 mg/L for the test item BHT amine (with 53.5% BHT) leads to a EC50 of 179 mg BHT/L
Key value for chemical safety assessment
- EC50 for microorganisms:
- 179 mg/L
Additional information
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