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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 14 Mar - 17 Mar 2000
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Justification for type of information:
- For the read-across justification see section 13.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Melting point: 63 °C
- Boiling point: 351 °C
- Water solubility: 0.82 mg/L (at 25 °C)
- log Pow: 5.64 - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 10 mg/L (nominal concentration)
- Sampling method: same volume of test water was sampled by each test vessel and mixed in each group at the test initiation and after exposure.
- Sample storage conditions before analysis: analysis was conducted immediately after sampling, no storage - Vehicle:
- yes
- Remarks:
- castor oil (HCO-50)
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: a stock solution (100 mg/L) was prepared with test substance, growth medium and dispersant. Stock solution was diluted with filter sterilized growth medium up to the concentration, and then filter sterilized.
- Controls: dilution water with/without vehicle
- Concentration of vehicle in test medium: stock solution: 1000 mg/L, final test solution: 100 mg/L
- Evidence of undissolved material: test water was colurless and no precipitate was observed. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Strain: ATCC-22662
- Source: American Type Culture Collection
- Age of inoculum: < 3 days
- Method of cultivation: on a rotary shaker
- Initial cell density: 1x104 cells/mL
ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions: same as test
- Any deformed or abnormal cells observed: not observed
- Temperature: 23+/-2ºC
- Light: 4000-5000 lux (continuous light)
- pH: no pH adjustments were made during exposure. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 23+/-2ºC
- pH:
- pH of the testing fluid at the start of exposure was 7.5-7.6; the pH at the end of the test was 8.4-9.1
- Nominal and measured concentrations:
- nominal concentration: 10.0 mg/L
measured concentration: 0 hour: 0.9 mg/L, 72 hour: < 0.8 mg/L (under detection limit) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 mL Erlenmeyer flask capped with air-permeable stoppers
- Type: open
- Material, fill volume: glass, 100mL
- Initial cells density: 10000 cells/mL
- Control end cells density: 1884000 ± 59400 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: continuously
- Light intensity and quality: 4000 - 5000 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter; 24, 48, 72 hours (measurements of the cell concentration were made by collecting 0.2 mL – 1.0 mL of the testing fluid from each test container, mixing it with electrolytes (Isotone II) so the total volume was 20mL, and measuring it with the Coulter Counter.)
TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: 10 mg/L is the highest concentration to be dissolved uniformly with solvent (100 mg/L)
- Range finding study: performed
- Test concentrations: 100 mg/L
- Results used to determine the conditions for the definitive study: EbC50: > 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (reagent grade)
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.9 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.9 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.9 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.9 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EbC50: 0.52 mg/L (72 h) - Reported statistics and error estimates:
- F & t-test
- Validity criteria fulfilled:
- yes
- Conclusions:
- It can be concluded that no toxic effects on aquatic algae for palmitic acid were observed up to the limit of water solubility.
Thus Reaction mass of stearic acid and stearic anhydride does not has to be classified.
Reference
Table 1: Growth inhibition of Selenastrum capricornutum
Initial Measured Concentration (mg/L) |
Biomass |
Growth rate |
Inhibition (%) |
Inhibition (%) |
|
Control |
- |
- |
Dispersant control |
-0.05 |
-0.31 |
0.9 |
-3.36 |
-1.51 |
Description of key information
It can be concluded that no toxic effects on aquatic algae for palmitic acid were observed up to the limit of water solubility.
Thus Reaction mass of stearic acid and stearic anhydride does not has to be classified.
The key values can be stated as:
EC50 > 0.9 mg/L
NOEC >= 0.9 mg/L
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.