Sodium 3-sulphonatopropyl acrylate

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17 Aug - 14 Sep 2001

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data based on the inhibition control of a ready biodegradability study. This approach is in accordance with the Guidance on information requirements and chemical safety assessment (Chapter R.7b: Endpoint specific guidance, ECHA 2014).

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: OECD 301D

GLP compliance:

yes

Remarks:

The Department of health of the Government of the United Kingdom

Test material

Test material information

Constituent 1

Reference substance name:

Potassium 3-sulphonatopropyl acrylate

EC Number:

250-465-0

EC Name:

Potassium 3-sulphonatopropyl acrylate

Cas Number:

31098-20-1

Molecular formula:

C6H10O5S.K

IUPAC Name:

potassium 3-(acryloyloxy)propane-1-sulfonate

Details on test material:

- Name of test material (as cited in study report): SPA (sulfopropyl acrylate)
- Physical state: powder
- Analytical purity: no data
- Lot/batch No.: 0200014/001
- Storage condition of test material: at room temperature in the dark

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 100 mg test substance were directly added to the culture medium (according to OECD 301D) and dissolved with the aid of ultrasonication (5 min). The volume was adjusted to 100 mL to give a 1000 mg/L stock solution which was inverted several times to ensure adequate mixing and homogeneity. An aliquot of 30 mL was dispersed into the final volume of 6 L to give a concentration of 5 mg/L. 1 drop inoculum per litre was added to each test flask.

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

- Source of inoculum/activated sludge: Final effluent of Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, United Kingdom, obtained on 17 Aug 2001
- Pretreatment: The effluent was filtered through coarse filter paper, while approx. the first 200 mL were discarded. The filtrate was maintained on continuous aeration in a temperature controlled room at 21 °C.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

14 d

Test conditions

Test temperature:

20°C

Nominal and measured concentrations:

5 mg/L (nominal)

Details on test conditions:

TEST CONDITIONS
- Composition of medium: 100 mg test substance were directly added to the culture medium (according to OECD 301D) and dissolved with the aid of ultrasonication (5 min). The volume was adjusted to 100 mL to give a 1000 mg/L stock solution which was inverted several times to ensure adequate mixing and homogeneity. An aliquot of 30 mL was dispersed into the final volume of 6 L to give a concentration of 5 mg/L. 1 drop inoculum per litre was added to each test flask.
- Test temperature: 20 °C
- pH adjusted: no

TEST SYSTEM
- Culturing apparatus: 250 - 300 mL darkened glass bottles (BOD bottles), stoppered
- Number of culture flasks/concentration: 2
- Measuring equipment: Yellow Springs oxygen meter and BOD Probe

SAMPLING
- Sampling frequency: after 0. 3, 6, 9, 12, 15, 18, 21, 24 and 28 d

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: -
- Toxicity control: yes, containing 5 mg/L test substance and 1.5 mg/L reference substance

Reference substance (positive control):

yes

Remarks:

Benzoic acid, sodium salt

Results and discussion

Effect concentrations

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Result of toxicity control from ready biodegradability test

Results with reference substance (positive control):

The reference substance is degraded by 74% within the 14-day window, confirming the inoculum and the test set-up to be appropriate.

Any other information on results incl. tables

The toxicity control was degraded by 27% within 14 d. Since the biodegradation was > 25 % the test substance is not considered to be inhibitory to the inoculum.

 

The test item concentration in the toxicity control of 5 mg/L can be used as NOEC value for the toxicity to aquatic microorganisms.