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EC number: 270-156-4 | CAS number: 68411-97-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A defined amount of the test substance (stock solution concentration = 500 mg/L) was weighed into a measuring flask and the total volume was filled up with dilution water. The test bottles were conditioned about 15 min before the test was started.
- Differential loading: yes
- Controls: yes, dilution water served as negative control - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Length at study initiation: 2.0 cm (average length)
ACCLIMATION
- Acclimation period: at least 14 d
- Feeding frequency during acclimation: daily
FEEDING DURING TEST
- Frequency: no feeding during the test - Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 0 h: 18.2 (control), 18.2 - 18.3 °C (treatments)
96 h: 18.2 (control), 18.1 - 18.2 °C (treatments) - pH:
- At 0 h: 7.8 (control), 7.6 - 7.8 (test solutions)
At 96 h: 7.7 (control), 7.7 - 7.8 (test solutions) - Dissolved oxygen:
- At 0 h: 8.1 mg/L (control), 7.4 - 7.9 mg/L (test solutions)
At 96 h: 8.1 mg/L (control), 6.9 - 7.5 mg/L (test solutions), corresponding to 86.5% of the air saturation - Nominal and measured concentrations:
- nominal: control, 1.0, 2.0, 5.0, 10.0 and 20.0 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: beakers
- fill volume: 7 L
- Aeration: yes, constantly
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: chlorine-free drinking water
- Hardness (dilution water): 37.4 mg/L Ca-content (between 4 - 100 mg/L)
- Copper content: < 0.01 mg/L
- Chlorine: none
- Culture medium different from test medium: no
- Intervals of water quality measurement: PH values, O2 concentration and temperature were measured at 0, 24, 48, 72 and 96 h
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12 h light-dark cycle
- Light intensity: indirect day-similar light
EFFECT PARAMETERS MEASURED:
The fish were inspected at 24, 48, 72 and 96 h.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - 2.5
- Range finding study: no - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3.16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC10
- Effect conc.:
- 1.76 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Mortality of control: 0%
- Effect concentrations exceeding solubility of substance in test medium: ?? - Reported statistics and error estimates:
- The statistical evaluation is based on a Probit analysis. The calculation of the Probit values with the date after 24 to 96 h bases was performed with with computer program "Dosis 50". In this program the experimentally determined percentages in the range from 1 - 99% can be used for the calculation of the Probit values and so for the evaluation of the effective concentrations. For the experimental values of 0% and 100% there are no Probit values defined. For the calculation of the Probit values of these percentages the percentages 1% and 99% were used.
- Sublethal observations / clinical signs:
The highest tested concentration of test substance producing no mortality under test conditions after 96 h was 1.0 mg/L.
The lowest tested concentration of test substance producing 100% mortality under test conditions after 96 h was 10.0 mg/L.
Table 1. Mortality in 24 h intervals.
Concentration of test substance
[mg/L]
Dead fish [%] after 24 h
Dead fish [%] after 48 h
Dead fish [%] after 72 h
Dead fish [%] after 96 h
20.0
100
100
100
100
10.0
71
100
100
100
5.0
29
71
71
86
2.0
14
14
14
14
1.0
0
0
0
0
control
0
0
0
0
- Validity criteria fulfilled:
- yes
- Remarks:
- No analytical measurement was conducted. Thus, the deviation from the nominal concentration could not be checked.
Reference
Description of key information
LC50 (96 h) = 3.16 mg/L test substance (nominal, Danio rerio, OECD 203)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 3.16 mg/L
Additional information
One GLP-study according to OECD 203 investigating the short-term toxicity of Glycine, N-methyl-, N-coco acyl derivatives (CAS 68411-97-2) to freshwater fish is available. The test organism Danio rerio was exposed to five nominal concentrations of the test substance ranging from 1.0 - 20.0 mg/L (1, 2, 5, 10 and 20 mg/L) in controlled conditions for 96 h. No analytical measurement was conducted. Mortality was recorded at daily intervals and was compared to the negative control at test termination. After 96 h mortality was observed at concentrations ≥ 2.0 mg/L. The derived 96 h nominal LC50 value was 3.16 mg/L.
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