Resin acids and Rosin acids, hydrogenated, sodium salts

Partition coefficient

Link to relevant study record(s)

Reference

Endpoint:

partition coefficient

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 117 (Partition Coefficient (n-octanol / water), HPLC Method)

Qualifier:

according to guideline

Guideline:

other: Council Regulation (EC) No. 266/2016, Method A.24, ”Partition Coefficient”

Principles of method if other than guideline:

Using the HPLC method for the estimation of the partition coefficient (POW) of Na salts of hydrogenated rosin (FORAL NC®).
The decision for the HPLC method was made due to the result of a non GLP preliminary testing (log POW of 5.75 and 7.58, respectively, for two test item signals).

GLP compliance:

yes

Type of method:

HPLC method

Partition coefficient type:

octanol-water

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Charge: CKS4107
- Expiration date of the lot/batch: 01-09-2019
- Purity test date: 100%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: 2-8°C under nitrogen and protected from light

Key result

Type:

log Pow

Partition coefficient:

6.89

Temp.:

21.9 °C

Remarks on result:

other: pH non available

Remarks:

pH non available

Details on results:

Please check the box "attached background infomation" for additional infomation

Conclusions:

The log Pow of Na salts hydrogenated rosin was determined to be 6.89 (weighted average mean value)

Executive summary:

The partition coefficient (n-octanol / water) of Na salts of hydrogenated rosin was determined according to the OECD 117 Guideline. The test was performed with a concentration of approx. 200 mg/L of the test material in 75% methanol: 25% water. Samples of the test item were analysed via HPLC on a C18 column with diode array detection. Calibration was performed using seven reference items with known log Pow values. The reference items were measured twice, once before and once after the measurement of the test item. The void time of the HPLC system was determined with sodium nitrate.

For each of the four test item signals, two individuals log Pow values were derived under consideration of the first and second calibration and a mean value was calculated from these individual log Pow values. The values for the fourth signal needed to be extrapolated.

The partition coefficient was determined at 21.9 °C to be 4.51 for the first signal, 5.80 for the second signal, 5.95 for the third signal and 7.80 for the fourth.

The value of the last signal needed to be extrapolated. The weighted average mean value of the log Pow for the test item was calculated to be 6.89.

Description of key information

The partition coefficient (n-octanol / water) of Na salts of hydrogented rosin was determined according to the OECD 117 Guideline. The test was performed with a concentration of approx.. 200 mg/L of the test material in 75% methanol: 25% water. Samples of the test item were analysed via HPLC on a C18 column with diode array detection. Calibration was performed using seven reference items with known log Pow values. The reference items were measured twice, once before and once after the measurement of the test item. The void time of the HPLC system was determined with sodium nitrate.

For each of the four test item signals, two individuals log Pow values were derived under consideration of the first and second calibration and a mean value was calculated from these individual log Pow values. The values for the fourth signal needed to be extrapolated.

The partition coefficient was determined at 21.9 °C to be 4.51 for the first signal, 580 for the second signal, 5.95 for the third signal and 7.80 for the fourth.

The value of the last signal needed to be extrapolated. The weighted average mean value of the log Pow for the test item was calculated to be 6.89.