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EC number: 214-290-3 | CAS number: 1119-94-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- other: Publication in literature
- Adequacy of study:
- other information
- Study period:
- 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Justification for type of information:
- Data considered acceptable for purpose of classification of an intermediate substance under SCC.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Reference
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- other: Publication in literature
- Adequacy of study:
- other information
- Study period:
- 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Justification for type of information:
- Data considered acceptable for purpose of classification of an intermediate substance under SCC.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- not indicated
- Deviations:
- yes
- Principles of method if other than guideline:
- - Daphnias were observed for 24 hours instead of 48 hours.
- No analytics of the test concentrations were reported. - GLP compliance:
- no
- Remarks:
- Publication in literature
- Analytical monitoring:
- not specified
- Vehicle:
- no
- Details on test solutions:
- The test substance was dissolved in Standard Reference Water (SRW) or distilled water.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia
- Source: Department of Biology, University of Turku
- Age at study initiation: <24 hours
- Feeding during test: no
ACCLIMATION: not specified
CULTURE CONDITIONS
- Medium: M4
- Temperature: 18-20°C
- Photperiod (hr light/hr dark): 12/12
- Feeding: yes, Scenedesmus algae, every second or third day depending on the density of the culture - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
- Remarks on exposure duration:
- An exposure period of 24 hours was determined based on the knowledge that toxicity of surfactants is usually expressed within the first 24 hours.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL polystyrene vessels, fill volume: 50 mL, closed with a polyethylene cap
- Aeration: no
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Standard Reference Water (SRW) according to OECD guideline.
- Culture medium different from test medium: yes
- Intervals of water quality measurement: not specified
OTHER TEST CONDITIONS: not specified
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilization was determined visually after 24 hours - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.37 mg/L
- Nominal / measured:
- not specified
- Basis for effect:
- mobility
- Details on results:
- - Results were expressed in micromolar: EC50 = 1.2 µM
- No abnormalities during the observations of the Daphnia were reported.
- No details were provided concerning the basis of the effect levels. Since also no analytics were reported, it is assumed that the effect level was based on the nominal concentrations. - Validity criteria fulfilled:
- not specified
- Conclusions:
- The EC50 for Dodecyltrimethylammonium bromide towards Daphnia magna was 0.37 mg/L.
- Executive summary:
The acute toxicity of Dodecyltrimethylammonium bromide towards Daphnia magna was assessed in a study available in public literature, performed similar to OECD guideline 202. Deviating from the guideline was the shorter exposure period (24 hours) based on the knowledge that toxicity of surfactants is usually expressed within the first 24 hours. Also, test solutions were not analysed for actual exposure concentrations. A range of five different concentrations was tested and a control was included. Twenty daphnids (<24 hours) were exposed to each test concentration. The study was repeated three times.
Immobilization was determined visually after 24 hours. Results showed that the EC50 for immobilization of the daphnids was 0.37 mg/L.
Data source
Reference
- Reference Type:
- publication
- Title:
- The Acute Toxicity of Surfactants on Fish Cells, Daphnia magna and Fish - A Comparative Study
- Author:
- Sandbacka, M., Christianson, I., Isomaa, B.
- Year:
- 2 000
- Bibliographic source:
- Toxicology in Vitro 14 (2000) pp. 61-68
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- Not specified
- Deviations:
- yes
- Principles of method if other than guideline:
- - Fish were exposed to the test substance for 24 hours instead of 96 hours (see 'Remarks on exposure duration').
- Test solutions were not anlaysed for actual exposure concentrations. - GLP compliance:
- no
- Remarks:
- Publication in literature
Test material
- Reference substance name:
- Dodecyltrimethylammonium bromide
- EC Number:
- 214-290-3
- EC Name:
- Dodecyltrimethylammonium bromide
- Cas Number:
- 1119-94-4
- Molecular formula:
- C15H34N.Br
- IUPAC Name:
- dodecyltrimethylazanium bromide
- Test material form:
- not specified
- Details on test material:
- Type of substance: synthetic detergent
Supplier: Sigma Chemical Co. (St. Lous, MO, USA)
Molecular formula: C15H34NBr
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- no
- Details on test solutions:
- No details provided.
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Source: obtained from a local fish farm
- Age at study initiation (mean and range, SD): not indicated
- Length at study initiation (length definition, mean, range and SD): 8.5 ± 0.4 cm
- Weight at study initiation (mean and range, SD): 6.6 ± 0.6 g
ACCLIMATION
- Acclimation period: 3 weeks
- Acclimation conditions:
* temperature: 12°C
* aeration: yes
* photoperiod (hrs light/hrs dark): 12/12
- Type and amount of food during acclimation: commercial rainbow trout pellets
- Feeding frequency during acclimation: daily
FEEDING DURING TEST: no
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
- Remarks on exposure duration:
- An exposure period of 24 hours was determined based on the knowledge that toxicity of surfactants is usually expressed within the first 24 hours and because the Daphnia test in the same study was performed with the same exposure period.
Test conditions
- Hardness:
- 49 mg CaCO3/L
- Test temperature:
- 11.3 ± 0.9°C
- pH:
- 7.8
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 10-litre glass aquaria
- Aeration: yes
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: well water
- Culture medium different from test medium: no
- Intervals of water quality measurement: not indicated
OTHER TEST CONDITIONS: not indicated
EFFECT PARAMETERS MEASURED: mortality after 24 hours - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrations
- Key result
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 40.6 mg/L
- Nominal / measured:
- not specified
- Basis for effect:
- mortality (fish)
- Details on results:
- - Results were expressed in micromolar: LC50 = 131.6 ± 3.3 μM
- No abnormalities during the observations of the fish were reported.
- No details were provided concerning the basis of the effect levels. Since also no analytics were reported, it is assumed that the effect level was based on the nominal concentrations.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The LC50 for Dodecyltrimethylammonium bromide towards rainbow trout was 40.6 mg/L.
- Executive summary:
The acute toxicity of Dodecyltrimethylammonium bromide towards rainbow trout was assessed in a study available in public literature, performed similar to OECD guideline 203. Deviating from the guideline was the shorter exposure period (24 hours) based on the knowledge that toxicity of surfactants is usually expressed within the first 24 hours. Also, test solutions were not analysed for actual exposure concentrations. A range of five different concentrations was tested and a control was included. Ten fish were exposed to each test concentration. The study was repeated three times.
Mortality was determined after 24 hours. Results showed that the LC50 for Dodecyltrimethylammonium bromide towards rainbow trout was 40.6 mg/L.
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