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EC number: 215-892-9 | CAS number: 1445-45-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Justification for type of information:
- justification for analogue approach: see IUCLID section 13 "Read-across justification"
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Details on sampling:
- Sampling method: a portion of a reaction mixture was transferred to a standard BOD dilution bottle.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock solutions (0.5 to 5.0 g/L) of the test chemical were prepared in deionized water. When necessary, the stock solutions were adjusted to pH 7.5 ± 0.5 by the addition of 1N H2SO4 or 1N NaOH as required - Test organisms (species):
- other: activated sludge from domestic and industrial sewage treatment plants
- Details on inoculum:
- Activated sludge was obtained on the day prior to the first day of each series of inhibition tests. On return to the laboratory, the solids were allowed to settle and the waste liquor was discarded. The solids were then transferred into a 9-liter laboratory-scale, semi-continuous activated sludge cylinder and diluted to approximately 8.5 liters with deionized water. The system was initially mixed by aerating at a rate of approximately 0.5 L/min, and the concentration of mixed liquor suspended solids was determined by gravimetric analysis. The solids were then allowed to settle in the cylinder and the upper layer of waste liquor was discarded. The activated sludge was washed three times with appropriate volumes of deionized water. After washing, the system was adjusted to contain 4000 ± 400 mg of mixed liquor suspended solids (dry weight) per liter. The system was then aerated continuously at a rate of 0.5 L/min and incubated at ambient temperature (21°C).
The system was supplemented daily with 50 mL of a synthetic sewage stock solution per litter of activated sludge. The synthetic sewage stock solution was composed of, per liter: Bacto-Peptone, 16.0 g; Bacto-Beef extract, 11.0 g; urea 3.0 g; K2HPO4, 28 g; MgSO4 • 7H2O, 0.2 g; CaCl2 • 2H2O, 0.4 g; and NaCl, 0.7 g. Final pH of the stock solution was adjusted to pH 7.0 with H3PO4. Fresh stock solution was prepared as required and stored for not more than two days at 5°C.
When the same source of inoculum was to be used for testing on a subsequent day (maximum of 7 days). an additional 50 mL of synthetic sewage stock solution was added per liter of activated sludge. The system was incubated overnight. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 21°C
- pH:
- 7.4 - 8.0
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 1L-bottle, final volume 500 mL
- Aeration: 0.5 - 1.0 L/min
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water
OTHER TEST CONDITIONS
- Adjustment of pH: yes, 7.5 ± 0.5 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorphenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- During a number of preliminary inhibition studies, it was observed that the pH of a test reaction often changed during the 3-hour incubation period. Thus, decreases in the respiration rate may have been due to a combination of both the effects of pH and toxicity of the test chemical. As a consequence, the buffering capacity of the synthetic sewage nutrient medium was increased to prevent changes in pH of reaction mixtures.
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: IC50 for 3,5-dichlorphenol: 12.2 ± 2.2 mg/L (18% variance) (municipal sewage), 11.4 ± 1.5 mg/L (13% variance) (industrial sewage) - Reported statistics and error estimates:
- Inhibition data were analyzed using Thompson's method of moving averages to estimate IC50 values. The experimental data were also analyzed using a probit-transformation model similar to that described by Larson and Schaeffer (1982). A nonlinear curve-fitting program (Procedure NLIN of SAS) was used to estimate IC50 values and associated 95% confidence intervals.
- Endpoint:
- toxicity to microorganisms
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP, non guideline study, published in peer reviewed literature, limitations in reporting but otherwise adequate for assessment
- Justification for type of information:
- justification for analogue approach: see IUCLID section 13 "Read-across justification"
- Reason / purpose for cross-reference:
- read-across source
- Principles of method if other than guideline:
- The effect of the test substance on the inhibition of the multiplication of bacterial cells of the genus Pseudomonas was investigated. Cultures of the test organisms were exposed to a series of test concentrations of the test substance for 16 hours and the effects on cell multiplication measured using a turbidimetric technique. Results are reported relative to the blank controls.
- GLP compliance:
- no
- Remarks:
- predates GLP
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No data reported - Analytical monitoring:
- no
- Details on sampling:
- The concentration of the bacterial suspension was measured turbidimetrically at 0 and 16 hours
- Vehicle:
- no
- Details on test solutions:
- Test solutions prepared from a stock solution, with each dilution containing 1 part v/v test substance solution in 2^0 to 2^14 v/v double distilled water. Nutrient medium, trace element solution and vitamin solution were added to each test vessel together with 10ml of bacterial suspension.
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- Stock cultures of the test strain kept on the nutrient for stock and preliminary cultures in agar slant tubes. New stock cultures prepared at 1 week intervals. The inoculated stock culture incubated at 25°C for 24h
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 16 h
- Post exposure observation period:
- None
- Hardness:
- No data reported
- Test temperature:
- 25°C
- pH:
- authors state that the solution of test substance was neutralised by the addition of a minimal volume of acid or alkaline solution
- Dissolved oxygen:
- No data reported
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- No data reported
- Details on test conditions:
- Four parallel dilution series in 300ml Erlenmeyer flasks, stoppered with cotton-lined plastic caps. Both inoculated and non-inoculated dilution series left at 25°C for 16h
- Reference substance (positive control):
- no
- Remarks:
- No data reported
- Key result
- Duration:
- 16 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2 850 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Details on results:
- After 16 hours the extinction of the monochromatic radiation at 436nm in a 10mm layer in the inoculated dilution series was measured. The lowest concentration having a mean extinction value ≥3 below the mean for non-toxic dilutions is reported as the EC3.
- Results with reference substance (positive control):
- No data reported
- Reported statistics and error estimates:
- No data reported
- Validity criteria fulfilled:
- no
- Remarks:
- non-standard test
- Conclusions:
- An 16h EC3 of 2850 mg/l has been reported for Pseudomonas putida . This can be considered to be the NOEC.
- Executive summary:
An 16h EC3 of 2850 mg/l has been reported for Pseudomonas putida. This can be considered to be the NOEC. Although this study is non-GLP and is non guideline, it is considered suitable for use as a key study. The study investigates the effects of the test substance on a standard test organism. A series of exposure concentrations were used and results reported as the inhibition of culture growth relative to controls.
Referenceopen allclose all
Description of key information
Trimethyl orthoacetate is rapidly hydrolysed to methanol (CAS no. 67 -56 -1) and methyl acetate, which further hydrolyses to acetic acid (CAS no. 64 -19 -7) as final reaction product in the presence of water (< 2.4 h at pH 4, 7 and 9 at 50°C). Under neutral (pH 7) and acedic (pH 4) conditions, the half-life was < 1 h. The measured degradation of TMOA at pH 7 and 50°C correspond to a half-life time well below one day under normal outdoor conditions. Accordingly, reliable data of the hydrolysis products methanol and acetic acid are used to address the endpoint, which is entirely appropriate to draw conclusions on the toxicity of Trimethyl orthoacetate to microorganisms.
1. Methanol
- Toxicity to microorganism: IC50 (3 h) > 1000 mg/L for activated sludge based on growth inhibition (OECD TG 209)
2. Acetic Acid
- Toxicity to microorganism: EC3 (16 h) = 2850 mg/L (= NOEC) for Pseudomonas putida based on growth inhibition (non-standard test, predates GLP)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
Concerning study data for acetic acid:
An 16h EC3 of 2850 mg/l has been reported for Pseudomonas putida. This can be considered to be the NOEC. Although this study is non-GLP and is non guideline, it is considered suitable for use as a key study. The study investigates the effects of the test substance on a standard test organism. A series of exposure concentrations were used and results reported as the inhibition of culture growth relative to controls.
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