Copper, diammine[5,5'-bi-1H-tetrazolato(2-)-kN1, kN1']

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

March 2011

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: other: GLP certificate is not present in the report (Reliability Guarantee present). IUCLID informations according to english translation of the Japanese report ( CERI: 2011-03-29)

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

no

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

mixture of sewage, soil and natural water

Remarks:

30 mg/L (as a suspended substance concentration). The suspended substance concentration of the active sludge was 3400 mg/L.

Details on inoculum:

Sludge (surface water of rivers, lakes, and sea inlets including topsoil, and sewage treatment facility return sludge) was collected from 10 locations in Japan according to the test method of 5 a), and active sludge (collection period: December 2010, Usage Start: January 5, 2011) that had been prepared and managed at this test facility were used. Synthesized sewage (glucose, peptone, di-hydrogen potassium phosphate were dissolved in purified water, and adjusted to a pH or 7.0±1.0), and the activated sludge used after 22 hours.

Duration of test (contact time):

ca. 28 d

Initial conc.:

ca. 100 mg/L

Based on:

test mat.

Remarks:

30 mg of the test sample were directly incorporated into the test flask (300 mL)

Parameter followed for biodegradation estimation:

O2 consumption

Parameter followed for biodegradation estimation:

DOC removal

Parameter followed for biodegradation estimation:

test mat. analysis

Remarks:

Detection of 5,5'-bi-1H-tetrazole; copper (water soluble and insoluble) and ammonia

Details on study design:

TEST CONDITIONS
Supportive Medium 5L were prepared at a proportion where 3 mL of the respective A, B, C, and D solutions (according to JIS K 0102-2010) were added to 1L of purified water
pH adjusted to 7.0
Tested Substance Concentration: 100 mg/L
Active Sludge Concentration: 30 mg/L (As a suspended substance concentration)
Test Fluid Volume: 300 mL
Test Fluid Culture Temperature: 25±1°C
Test Fluid Culture
Time: 28 days (light-shielded)
Stirring method: rotating agitation with a stirrer

TEST SYSTEM
Closed-system Oxygen Demand Equipment
Thermostatic Chamber Data Processing Equipment
Test FluidAbiotic control to 100 mg/l
(Water + Tested Material): 1 Test Container [1]
300 mL of purified water and 30 mg of the tested substance were entered into a test container. Test suspension to 100 mg/L
(Sludge + Tested Material): 3 Test Containers [2][3][4]
The supportive medium [amount of the added active sludge volume (2.65 mL) subtracted from 300 mL] as well as the test sample 30 mg were entered into the test container. Procedure control to 100 mg/L
(Sludge + Aniline): 1 Test Container [6]
The supporting medium [amount with the added active sludge volume (2.65 mL) subtracted from 300 mL] and aniline 29.5 µL (30 mg) were entered into the test container so that the aniline concentration was 100 mg/L. The aniline was added after fractionation with a micro syringe.
Sludge Blank: 1 Test Container [5]
The supporting medium [amount with the added active sludge volume (2.65 mL) subtracted from 300 mL] and was entered into the test container

Reference substance:

aniline

Remarks:

100 mg/L (CAS number: 62-53-3; supplier: Wako Pure Chemical Industries; lot number: EPL1617)

Preliminary study:

From the results of the preliminary studies, it was determined that the solubility of the test material was very low in general-purpose solvents, and that 5,5'-bi-1H-tetrazole, copper and ammonia were liberated from the tested material in the test solution, and therefore it would be difficult to apply it to analysis in the tested product state. Thus, after the completion of the culturing period, a quantitative analysis was performed on the 5,5'-bi-1H-tetrazole, copper, and ammonia constituting the tested substance in order to estimate the biodegradation of the test material.

Key result

Parameter:

% degradation (O2 consumption)

Value:

ca. 3

Sampling time:

28 d

Remarks on result:

other: continuous measurements

Key result

Parameter:

other: Detection rate of 5,5'-bi-1H-tetrazole

Value:

0

Sampling time:

28 d

Remarks on result:

other: Detection rate for water-soluble 5,5'-bi-1H-tetrazole: 59% and for water insoluble 5,5'-bi-1H- tetrazole: 40%.

Key result

Parameter:

other: Detection rate of copper

Value:

0

Sampling time:

28 d

Remarks on result:

other: Detection rate of water soluble copper: 0% and water insoluble copper: 97.7%

Key result

Parameter:

other: Detection rate of ammonia

Value:

ca. 0

Sampling time:

28 d

Remarks on result:

other: Detection rate of ammonia: 67.7%

Key result

Parameter:

% degradation (test mat. analysis)

Remarks on result:

other: Quantitative analysis of the test material can not be performed, the degradation by direct analysis of the tested material was not calculated.

Details on results:

Average percentage detection of DOC: 42% (sludge + test material); 66% (water + test material)
These results implied that part of the 5,5'-bi-1H-tetrazole was not liberated from the tested material.

Average percentage detection of water-soluble 5,5'-bi-1H-tetrazole: 59% (sludge + test material); 64% (water + test material)

Average percentage detection of water insoluble 5,5'-bi-1H-tetrazole: 40% (sludge + test material); 36% (water + test material)
Thereby confirming that structural changes did not occur in the 5,5'-bi-1H-tetrazole after 28 days of incubation.

Average percentage detection of water-soluble copper: 0% (sludge + test material); 6% (water + test material)

Average percentage detection of water insoluble copper: 97.7% (sludge + test material); 97% (water + test material)
All copper constituting the tested material was released into the water and resides in an insoluble inorganic state.

Average percentage detection of ammonia: 67.7% (sludge + test material); 95% (water + test material).

Results with reference substance:

% biodegradation BOD 28 d: 72%% biodegradation DOC 28 d: Not available

Percentage biodegradation of reference and test material (O2 consumption):

Vessel	7th day	7th day	14th day	14th day	21st day	21st day	28th day	28th day	Mean
No.	BOD (mg)	Deg. (%)	BOD (mg)	Deg. (%)	BOD (mg)	Deg. (%)	BOD (mg)	Deg. (%)	Deg. (%)
[6]	57.2	61	67.9	71	71.6	72	71.6	72
[5]	2.0	-	4.0	-	6.7	-	6.7	-
[2]	1.7	-1	4.5	1	9.0	4	9.0	4	3
[3]	1.6	-1	5.4	2	9.3	5	9.3	5
[4]	1.5	-1	3.1	-2	6.0	-1	6.2	-1
[1]	0.0	-	0.3	-	1.0	-	1.0	-

 

[1]: water + test material

[2] [3] [4]: sludge + test material

[5]: control blank

[6]: sludge + aniline

Validity criteria fulfilled:

yes

Remarks:

Difference between extremes of replicates values of % biodegradation (by BOD): 6% (< 20%); % biodegradation of aniline by BOD: after 7 days 61% (> 40%) and after 14 days 71% (> 65%); BOD value of control blank (after 28 days): 6.7 mg (< 60 mg/L)

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

Report conclusion: Under these test conditions, 5,5'-bi-1H-tetrazole and ammonia were abiotically liberated from part of the tested material, and water insoluble inorganic copper was produced.In addition, the 5,5'-bi-1H-tetrazole corresponding to the organic part of the tested material also included the liberated part and was not biodegraded.

Executive summary:

5,5'-bi-1H-tetrazole and ammonia were abiotically liberated from part of the tested material, and water insoluble inorganic copper was produced. In addition, the 5,5'-bi-1H-tetrazole corresponding to the organic part of the tested material also included the liberated part and was not biodegraded.

Under test condition no biodegradation is observed.

Description of key information

5,5'-bi-1H-tetrazole and ammonia were abiotically liberated from part of the tested material, and water insoluble inorganic copper was produced. In addition, the 5,5'-bi-1H-tetrazole corresponding to the organic part of the tested material also included the liberated part and was not biodegraded.

Under test condition no biodegradation is observed.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed