Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-024-4 | CAS number: 113-24-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Version / remarks:
- 2002
- Deviations:
- no
- GLP compliance:
- not specified
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Methyl pyruvate
- EC Number:
- 209-987-4
- EC Name:
- Methyl pyruvate
- Cas Number:
- 600-22-6
- Molecular formula:
- C4H6O3
- IUPAC Name:
- methyl 2-oxopropanoate
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA
- Sex:
- not specified
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 7-12 weeks
Study design: in vivo (LLNA)
- Vehicle:
- acetone/olive oil (4:1 v/v)
- Concentration:
- 1, 2.5, 5 and 10%
- No. of animals per dose:
- 4
- Details on study design:
- ANIMAL ASSIGNMENT AND TREATMENT
- Criteria used to consider a positive response: A substance was classified as a skin sensitizer, if, at one or more test concentrations, it induced a 3-fold or greater increase in LNC proliferative activity compared with concurrent vehicle-treated controls.
TREATMENT PREPARATION AND ADMINISTRATION: Mice were treated topically on the dorsum of both ears with 25 µL of test material or with an equal volume of the vehicle (AOO) alone. Treatment was performed daily for 3 consecutive days.
Results and discussion
In vivo (LLNA)
Resultsopen allclose all
- Key result
- Parameter:
- EC3
- Value:
- 2.4
- Parameter:
- SI
- Value:
- 1.2
- Test group / Remarks:
- concentration: 1 %
- Parameter:
- SI
- Value:
- 2.3
- Test group / Remarks:
- concentration: 2.5 %
- Parameter:
- SI
- Value:
- 4.7
- Test group / Remarks:
- concentration: 5 %
- Parameter:
- SI
- Value:
- 8
- Test group / Remarks:
- concentration: 10 %
- Cellular proliferation data / Observations:
- EC3 CALCULATION
The EC3 value was calculated by interpolating between 2 points on the SI axis, one immediately above and one immediately below the SI value of 3. The vehicle-treated control value (by definition, SI = 1) cannot be used for the latter. Where the data points lying immediately above and below the SI value of 3 have the co-ordinates (a, b) and (c, d), respectively, then the EC3 value may be calculated using the equation: EC3 = C + [3 - d/b - d](a - c)
Applicant's summary and conclusion
- Interpretation of results:
- Category 1B (indication of skin sensitising potential) based on GHS criteria
- Conclusions:
- The test item was determined to have a skin sensitising potential (GHS Category 1B).
- Executive summary:
A local lymph node assay was performed according to OECD guideline 429 to assess the skin sensitising potential of the test item. The test was performed with four CBA mice per dose group. As vehicle acetone/olive oil (4:1) was used. The mice were treated topically in the dorsum with 25 µL of the test item (1, 2.5, 5 or 10 %.) or the vehicle alone. Treatment was performed daily for 3 consecutive days. 5 days following the initiation of exposure, all mice were injected via the tail vein with 250 µL of phosphate-buffered saline (PBS) containing 20 µCi of tritiated thymidine. Mice were sacrificed 5h later, and the draining lymph nodes excised and pooled for each experimental group. The determined stimulation indices were 1.2 (1 %), 2.3 (2.5 %), 4.7 (5 %) and 8.0 (10 %) and the determined EC3 value was 2.4. Therefore, the test item was determined to have a skin sensitising potential (GHS Category 1B).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.