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EC number: 603-020-3 | CAS number: 12501-23-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-03-22 to 2017-04-21
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- 2010
- Deviations:
- no
- Principles of method if other than guideline:
- There were no deviations from the guideline.
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Batch no: 160306
- Purity: > 99 % - Analytical monitoring:
- no
- Details on sampling:
- Not applicable
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: direct addition by initial weight into the test flasks; test substance was weighed in the required amounts for the test concentrations directly to the test vessels
- Controls: negative control, procedure control
- Evidence of undissolved material: no
- Replicates:
6 replicates for the negative control,
3 replicates for each test substance concentration,
2 replicates for each reference substance concentration - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: wastewater treatment plant of Mannheim, Germany; collected on 22 Mar 2017; arrival in the test facility: 22 Mar 2017
- Preparation of inoculum for exposure:
After arrival of the activated sludge suspension in the test facility the suspension was sieved
with a fine woven mesh (mesh size about 1 mm). This suspension was pre-aerated over
night at room temperature. At the next day the sludge suspension was washed once with
drinking water and the suspension was adjusted to 3 g/L Dw.
- Initial biomass in the test vessel: 1.5 g/L Dw - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- No
- Test temperature:
- Test item (after incubation): 21.0 – 21.8 °C
- pH:
- Test item: 7.7-8.0
- Nominal and measured concentrations:
- Nominal: 1000, 500, 250, 125, 62.5 mg/L based on test substance without correction of purity and blank controls
- Details on test conditions:
- - Incubation time: 3 hours
- Test temperature: 21.0 – 21.8 °C
- Test vessels: glas-beakers (nominal volume 1L)
- Test volume: 500 mL
- Synthetic medium: 16 mL/test vessel of 100-fold concentrated OECD medium
- Oxygen concentration during aeration: > 2 mg/L
- Oxygen concentration immediately before measurement: > 7 mg/L
- Duration of the measurement of oxygen consumption: about 8 - 10 min (one replicate 10.1 min)
- Sludge concentration in the test vessel: 1.5 g/L Dw - Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - Any observations that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no
- Adsorption: no
VALIDITY
The coefficient of variation of the six replicates of blank control was 5.4 % O2 consumption.
The EC50 of 3,5-dichlorophenol was 7.4 mg/L
The mean oxygen uptake of the blank controls was 21 mg/g*h
The test conformed to all validity criteria and is valid. - Results with reference substance (positive control):
- The EC50 of the reference substance 3,5-dichlorophenol was in the range of 2-25 mg/L in 3 hours.
- Reported statistics and error estimates:
- The consumption rates were used for the determination of the ECx for the reference substance by the probit method based on Finney with the software TOXRAT Professional 2.10. The effect concentrations were given with an accuracy of 2 significant digits.
- Validity criteria fulfilled:
- yes
Reference
Table: Measured data of oxygen content of the test assays at the start and end of the evaluation time
Assay identification | BC1 | BC2 | BC3 | BC4 | BC5 | BC6 | RS1 | RS2 | RS3 | RS4 | RS5 | RS6 |
O2 conc. Start [mg/L] | 7.2 | 6.5 | 6.8 | 6.9 | 6.8 | 8.0 | 7.1 | 7.7 | 8.1 | 8.7 | 9.2 | 9.2 |
O2 conc. End [mg/L] | 4.3 | 3.5 | 3.5 | 3.6 | 3.4 | 4.8 | 4.2 | 4.8 | 6.7 | 7.3 | 9.0 | 9.0 |
O2 consumption rate [mg/L] | 2.9 | 3.0 | 3.3 | 3.3 | 3.4 | 3.2 | 2.9 | 2.9 | 1.4 | 1.4 | 0.2 | 0.2 |
O2 concumption rate in minutes | 6.0 | 6.0 | 6.5 | 6.0 | 6.1 | 6.2 | 6.0 | 6.5 | 6.3 | 6.5 | 5.0 | 5.0 |
O2 consumption rate [mg/L×h], Rτ | 29 | 30 | 30 | 33 | 33 | 31 | 29 | 27 | 13 | 13 | 2 | 2 |
Specific O2 consumption rate [mg/g×h] | 19 | 20 | 20 | 22 | 22 | 21 | 19 | 18 | 9 | 9 | 1 | 1 |
Calculation of inhibition respiration [%], Iτ | - | 6 | 13 | 58 | 58 | 94 | 94 |
Legend: RS = reference substance, BC = blank control
The respiration rate was calculated in mg O2/L×h. The oxygen consumption was measured over a period of 8-10 minutes. 5 - 6.5 minutes from the plotted track within a linear range of the graph was used for the determination of the respiration rate.
Description of key information
The 3-hour EC10 and EC50, respectively, of the test item was determined to be > 1000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
The tocxicity to aquatic microorganisms was assessed in a Activated Sludge Respiration Inhibition Test in compliance with GLP criteria and OECD Guideline 209. Activated sludge from a municipal wastewater treatment plant of Mannheim (Germany) was exposed for 3 hours to test item concentrations of 1000, 500, 250, 125, 62.5 mg/L (nominal, based on test substance without correction of purity and blank controls). The test substance was added in the required amounts according to the test concentrations directly to the test vessels (glass beakers, volume 1L) with 234 mL deionized water. After addition of 250 mL of inoculum suspension (3 g/L DW) the incubation was started by aeration of the test vessels with pressured air. The vessels for the blank control assays were prepared to the same procedure without addition of test- or reference substance. 6 replicates for the blank control, 3 replicates for each test substance concentration and 2 replicates for each reference substance concentration were prepared. No abiotic control was tested. After 3 hours incubation at 20 ± 2°C the mixtures in the test vessels were placed subsequently for oxygen measuring. The oxygen uptake was measured for a period of about 8 to 10 minutes (one replicate: 10.1 min). The oxygen concentration during aeration was > 2 mg/L and immediately before measurement > 7 mg/L. In result, the 3-hour EC10 and EC50 of the test item was determined to be both > 1000 mg/L. In conclusion, no monotone concentration response exists because no inhibition was determined. Therefore the ECx calculation is not meaningful and the EC10 value is assumed to be greater than 1000 mg/L. The test conformed to all validity criteria and is valid, since the coefficient of variation of the six replicates of blank control was 5.4 % O2 consumption, the EC50 of the reference substance 3,5-dichlorophenol was 7.4 mg/L and the mean oxygen uptake of the blank controls was 21 mg/g*h.
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